4,106 results
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pHAGE-EGFR
Plasmid#116731PurposeLentiviral expression of EGFRDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ATR Kinase dead
Plasmid#31612DepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationK2327R (kinase dead)PromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
mKO2-N1
Plasmid#54625PurposeLocalization: N1 Cloning Vector, Excitation: 551, Emission: 565DepositorTypeEmpty backboneUseTagsmKO2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-beta-catenin-IRES-GFP
Plasmid#14717DepositorInsertbeta-catenin (Ctnnb1 Mouse)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GP
Plasmid#60495PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP
Plasmid#40640DepositorInsertNICD3 (NOTCH3 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLH-spsgRNA2
Plasmid#64114PurposeVector for expression of Sp sgRNA2 in mammalian cellsDepositorInsertSp sgRNA2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
DRH334: FCK-QuasAr2-mO2
Plasmid#51692PurposeFluorescent reporter of membrane voltage with improved sensitivityDepositorInsertQuasAr2-mOrange
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCaMKIIaAvailable sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-ROC1
Plasmid#19897DepositorInsertRING finger protein 1 (ROC1) (RBX1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry
Plasmid#26975PurposeAAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F1
Plasmid#24225PurposeMammalian expression of human E2F1 with HA tagDepositorInsertE2F1 (E2F1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionTagsExpressionWormMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FlagMLL-pl-ENL(5613)
Plasmid#20873DepositorUseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-Talin1
Plasmid#26724DepositorInsertTalin (Tln1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_C1
Plasmid#85044PurposeMammalian expression of mScarlet-iDepositorHas ServiceCloning Grade DNAInsertmScarlet-i
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIV-dTomato
Plasmid#21374Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomatoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GPHN.FingR-eGFP-CCR5TC
Plasmid#46296DepositorInsertGPHN.FingR-eGFP-CCR5TC (GPHN Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP11
Plasmid#22566DepositorInsertUSP11 (USP11 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR
Plasmid#112159PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A
Plasmid#28047DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
piRFP
Plasmid#31857Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorHas ServiceCloning Grade DNAInsertiRFP
UseTagsExpressionMammalianMutationT2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…PromoterCMVAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR(T/A)
Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MaLionR
Plasmid#113908PurposeRed ATP sensorDepositorInsertMaLionR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ack
Plasmid#62654PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expressionDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCL-CTIG
Plasmid#14901Purpose3rd generation lentiviral vector expressing tTA-VP16 and EGFP.DepositorInsertCMV-tTA-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorInsertActin (ACTB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Cas9-NLS-6xHis
Plasmid#62933PurposeExpression of Cas9-NLS-6xHis in bacterial cellsDepositorInsertCas9-NLS-6xHis
UseTags6xHis tag and NLSExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mNeonGreen
Plasmid#98876PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsert4xmts
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GFP-Fishell-1
Plasmid#83900PurposeGFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserteGFP
UseAAVTagsExpressionMutationPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP955
Plasmid#104164PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-N (N SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA6A-EGFR WT
Plasmid#42665DepositorInsertEGFR (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-HDAC4
Plasmid#45636DepositorInsertHDAC4 (HDAC4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-N1
Plasmid#54594PurposeLocalization: N1 Cloning Vector, Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
FU-tetO-Gateway
Plasmid#43914DepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…Tags6xHis and V5ExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-hMcl-1
Plasmid#25392DepositorInsertMCL1 (MCL1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-14-3-3ζ
Plasmid#48798Purposeexpresses human myc-14-3-3ζ in mammalian cellsDepositorInsertYWHAZ (YWHAZ Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV1-hMD2
Plasmid#13028DepositorInsertMD2 (LY96 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-VN155(I152L)
Plasmid#27097DepositorInsertVN155(I152L)
UseTagsMycExpressionMammalianMutationI152LPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
UseTagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKOV
Plasmid#25769DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CD68 promoter/enhancer
Plasmid#34837DepositorInsertCD68 promoter/enhancer -680/+140 (CD68 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2012AvailabilityAcademic Institutions and Nonprofits only
