4,146 results

1. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-puro-cMyc

   Plasmid

   #46970

   Purpose

   lentiviral expression of c-Myc

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C2-NLRP3

   Plasmid

   #73955

   Purpose

   Express EGFP-tagged human NLRP3 in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

   Insert

   NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV iE

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSGAb-km

   Plasmid

   #121999

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   F74G

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mCardinal

   Plasmid

   #51311

   Purpose

   Expresses far-red fluorescent protein mCardinal in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mCardinal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. hAAVS1 1R TALEN

   Plasmid

   #35432

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1R TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. hAAVS1 1L TALEN

   Plasmid

   #35431

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1L TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC-GAL4

   Plasmid

   #24344

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX APP Swe/Ind

    Plasmid

    #30145

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    human APP 695 Swedish/Indiana mutation (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Swedish and Indiana; K595N, M596L and V642F

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCN DSB Repair Reporter (DRR)

    Plasmid

    #98895

    Purpose

    DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-ISce1-ISce1-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB18cmBFPAzurite

    Plasmid

    #14034

    Depositor

    Patrick Daugherty

    Article

    Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

    Insert

    Azurite
    

    Use

    Yeast/bacteria optimized codons

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cbh

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-CMV mCherry-GFP-LC3B WT

    Plasmid

    #123230

    Purpose

    Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_BE4max

    Plasmid

    #112093

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest-566

    Plasmid

    #11517

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT3-EF1A-MYC-IRES-luc

    Plasmid

    #129775

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL-TRBO

    Plasmid

    #80082

    Purpose

    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-Foxo1

    Plasmid

    #17551

    Depositor

    Domenico Accili

    Article

    Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

    Insert

    pEGFP-N1 Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Stephen Ekker, Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Expression

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mCherry-Tsg101

    Plasmid

    #21505

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    Tsg101 (TSG101 Human)
    

    Use

    Tags

    Cherry*

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti CMV Neo DEST (705-1)

    Plasmid

    #17392

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Use

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge