175,573 results
-
-
p2xFLAGhYAP1
Plasmid#17791DepositorAvailable SinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
Klotho (secreted)
Plasmid#17713DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
PGKneotpAlox2
Plasmid#13444DepositorInsertPGKneotpAlox2
UseCre/LoxExpressionMammalianAvailable SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-GFP_2.0
Plasmid#11375PurposeRetroviral construct for miRNA expressionDepositorTypeEmpty backboneUseRNAi and RetroviralExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
1066 pBabe puroL PTEN
Plasmid#10785DepositorAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRExpressionMammalianAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralPromotertetONAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyAvailable SinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)
Plasmid#107941PurposeMammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutionsDepositorInserthuman codon optimized enAsCas12a (E174R/S542R/K548R)
Tags3x HA and NLS (nucleoplasmin)ExpressionMammalianMutationE174R, S542R and K548RPromoterCAGAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Full length Importin beta
Plasmid#106941PurposeTransient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)DepositorInserthuman Importin beta-1 (KPNB1) (KPNB1 Human)
TagsGFPExpressionMammalianPromoterCMV promoterAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TFEB-WT-MYC (#647)
Plasmid#99955Purposemammalian expressionDepositorAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_alphaTubulin_C1
Plasmid#85045PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(Hygro) myc-hPolQ-Flag
Plasmid#73132PurposeTo express in mammalian cells human PolQDepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP7_mCherry
Plasmid#61763PurposeExpresses PP7 coat protein fused to mCherryDepositorInsertPP7 coat protein fused to mCherry
UseLentiviralTagsmCherryExpressionMammalianPromoterUbc promoterAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Autobow
Plasmid#45182DepositorInsertpCAG-Autobow
ExpressionMammalianPromoterCAGAvailable SinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
tetO-ALN
Plasmid#43918DepositorUseLentiviral; Doxycycline inducibleTags6xHis and V5ExpressionMammalianMutationGylcine-serine-glycine (GSG) linker followed by v…PromoterTRE promoter, Tet-ONAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
AP2u2-mCherry
Plasmid#27672DepositorAvailable SinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GTB
Plasmid#26197DepositorInsertEnhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusMutationTVA, rabies glycoprotein are mammalian codon opti…Available SinceNov. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP
Plasmid#19444DepositorInsertGFP
ExpressionMammalianAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Cre-HA
Plasmid#16583DepositorAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPIASy
Plasmid#15208DepositorAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA 14-3-3 sigma
Plasmid#11946DepositorAvailable SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro
Plasmid#141383PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialPromoterT7Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianPromoterPGKAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX302_FOXA1-V5
Plasmid#70090PurposeExpression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vectorDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Lifeact-7
Plasmid#54694PurposeLocalization: Actin, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertLifeact-Dendra2
ExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
DRH296: FCK-Optopatch2
Plasmid#51694PurposeCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiologyDepositorInsertOptopatch2
UseLentiviralExpressionMammalianPromoterCaMKIIaAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-tdTomato
Plasmid#44332DepositorHas ServiceAAV5InserttdTomato
UseAAV; Adeno-associated virus ()ExpressionMammalianAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer
Plasmid#41584DepositorAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
LeGO-C2
Plasmid#27339DepositorInsertSFFV promoter, mCherry
UseLentiviralExpressionMammalianAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 IKKe flag
Plasmid#26201DepositorAvailable SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
-
pCAGMKOSiE
Plasmid#20865DepositorInsertmyelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
ExpressionMammalianAvailable SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2FLAG
Plasmid#16331DepositorInsertFlag tag
UseVertebrate expressionTagsFlagMutationFlag tag inserted into pCS2+ polylinkerAvailable SinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro largeTcDNA
Plasmid#14088DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET Nucleolin
Plasmid#13037DepositorAvailable SinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-ACTR3
Plasmid#8462DepositorAvailable SinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only
