Skip to main content
Addgene
Showing: 2451 - 2500 of 159111 results

  12. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    13425-plasmid-map-sequence-id-360003

    Enlarge

  13. pMM403_CaMKII-promoter

    Plasmid
    #34926
    Depositor
    Mark Mayford
    Insert
    CaMKII promoter (Camk2a Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKII
    Available since
    Aug. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    34926-plasmid-map-sequence-id-122246

    Enlarge

  14. TFORF0885

    Plasmid
    #142210
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARG (PPARG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142210-plasmid-map-sequence-id-397707

    Enlarge

  15. HA-tagged Bcr

    Plasmid
    #38189
    Depositor
    Nora Heisterkamp
    Insert
    BCR (BCR Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
    38189-plasmid-map-sequence-id-453534

    Enlarge

  17. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid
    #135281
    Purpose
    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-CDK12 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135281-plasmid-map-sequence-id-267208

    Enlarge

  27. FABP1A

    Plasmid
    #42344
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FABP1A (FABP1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. TFORF3131

    Plasmid
    #144607
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR5A2 (NR5A2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144607-plasmid-map-sequence-id-346763

    Enlarge

  30. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

    Enlarge

  31. pLNCX chick src Y527F

    Plasmid
    #13660
    Depositor
    Joan Brugge
    Insert
    src Y527F (SRC Chicken)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Y527F. Constitutive active mutant.
    Promoter
    Available since
    Jan. 4, 2007
    Availability
    Academic Institutions and Nonprofits only
    13660-plasmid-map-sequence-id-155869

    Enlarge

  34. ZJOM90

    Plasmid
    #133647
    Purpose
    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-SS-mCherry-HDEL
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133647-plasmid-map-sequence-id-292102

    Enlarge

  43. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid
    #125146
    Purpose
    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter
    Depositor
    Christopher Richie
    Insert
    HA-tagged hM4D(Gi) (CHRM4 Human)
    Use
    AAV and Cre/Lox
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    SYN1
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125146-plasmid-map-sequence-id-244357

    Enlarge

  47. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    David Sabatini, Eric Lander
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

    Enlarge

  50. AiP15072 - pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA (Alias: CN5072)

    Plasmid
    #230402
    Purpose
    AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available since
    Feb. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230402-plasmid-map-sequence-id-462407

    Enlarge

Showing: 2451 - 2500 of 159111 results