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177,145 results

Showing: 2451 - 2500 of 177145 results

  2. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Publication
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    137729-plasmid-map-sequence-id-272646

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  4. pEGFP-N1 Full length Importin beta

    Plasmid
    #106941
    Purpose
    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)
    Depositor
    Patrizia Lavia
    Publication
    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Importin beta-1 (KPNB1) (KPNB1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    106941-plasmid-map-sequence-id-204312

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  6. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    92062-plasmid-map-sequence-id-180159

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  11. GFP-BRD2

    Plasmid
    #65376
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRD2 (BRD2 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    65376-plasmid-map-sequence-id-261178

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  25. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6b
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    207852-plasmid-map-sequence-id-408170

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  28. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    107941-plasmid-map-sequence-id-418295

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  31. C1(1-29)-miniTurbo-V5_pCDNA3

    Plasmid
    #107174
    Purpose
    expresses V5-tagged miniTurbo on the mammalian ER membrane
    Depositor
    Alice Ting
    Publication
    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniTurbo (BirA mutant)
    Expression
    Mammalian
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    May 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    107174-plasmid-map-sequence-id-205307

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  32. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    15 Articles
    99376-plasmid-map-sequence-id-191027

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  35. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    70708-plasmid-map-sequence-id-364684

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  37. pCAG-VSVL

    Plasmid
    #64085
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene
    Depositor
    Ian Wickersham
    Insert
    VSV polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    15 Articles
    64085-plasmid-map-sequence-id-429028

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Showing: 2451 - 2500 of 177145 results