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167,150 results

Showing: 2121 - 2160 of 167150 results

  5. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

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  7. TFORF0460

    Plasmid
    #142631
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB5 (CREB5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142631-plasmid-map-sequence-id-397780

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  8. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid
    #107738
    Purpose
    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.
    Depositor
    Rylan Larsen
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre, dTomato
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107738-plasmid-map-sequence-id-207220

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  11. TFORF2541

    Plasmid
    #142399
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX5 (SOX5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142399-plasmid-map-sequence-id-397181

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  13. pRS416-yZ3EV-Z3pr (RB3578)

    Plasmid
    #69099
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    Expression
    Yeast
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69099-plasmid-map-sequence-id-122355

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  15. TFORF3549

    Plasmid
    #145025
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145025-plasmid-map-sequence-id-419572

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  23. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  24. TFORF0531

    Plasmid
    #143039
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PAX6 (PAX6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143039-plasmid-map-sequence-id-399061

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  39. pCIneo-hCHD7

    Plasmid
    #89458
    Purpose
    constitutive expression of human CHD7 in mammalian cells
    Depositor
    Peter Scacheri
    Insert
    CHD7 (CHD7 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89458-plasmid-map-sequence-id-176093

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  40. pGL4.23-MYC

    Plasmid
    #86461
    Purpose
    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.
    Depositor
    Eric Lander
    Article
    Fulco et al Science. 2016 Sep 29. pii: aag2445.
    Type
    Empty backbone
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    MYC
    Available Since
    Sept. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86461-plasmid-map-sequence-id-193100

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Showing: 2121 - 2160 of 167150 results