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165,750 results

Showing: 2121 - 2160 of 165750 results

  1. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  18. TFORF2932

    Plasmid
    #144408
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2A (TFAP2A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144408-plasmid-map-sequence-id-353338

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  20. Hs.NRAS Q61R

    Plasmid
    #83179
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61R
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83179-plasmid-map-sequence-id-158142

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  22. p426_Cas9_gRNA-ARS208a

    Plasmid
    #87383
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87383-plasmid-map-sequence-id-193425

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  24. AAV-DIO-SPOTlight-U2GCR

    Plasmid
    #231889
    Purpose
    Cre-dependent reporter to measure ISR state-dependent protein synthesis
    Depositor
    Nicole Calakos
    Insert
    DIO-SPOTlight-U2GCR
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231889-plasmid-map-sequence-id-460233

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  25. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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  33. 5KKP (PUS7)

    Plasmid
    #101878
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PUS7 (PUS7 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101878-plasmid-map-sequence-id-194704

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  35. pcDNA3-Lyso-AktAR2

    Plasmid
    #64933
    Purpose
    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-AktAR2 (LAMP1 Budding Yeast, Synthetic, Human)
    Tags
    Cerulean3, Full-length LAMP1, and cpVenus[E172]
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64933-plasmid-map-sequence-id-109712

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  39. mCherry-MAP4-C-10

    Plasmid
    #55075
    Purpose
    Localization: Microtubules, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MAP4 (Map4 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 640-1045 of MAP4
    Promoter
    CMV
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2121 - 2160 of 165750 results