Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,777 results

Showing: 2701 - 2750 of 163777 results

  1. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Promoter
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

    Enlarge

  5. EF1a mCherry

    Plasmid
    #129431
    Purpose
    expression of mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129431-plasmid-map-sequence-id-256111

    Enlarge

  6. MSCV-IRES-Thy1.1 DEST

    Plasmid
    #17442
    Purpose
    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction
    Depositor
    Anjana Rao
    Article
    Wu et al Cell. 2006 Jul 28. 126(2):375-87.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 20, 2008
    Availability
    Academic Institutions and Nonprofits only
    17442-plasmid-map-sequence-id-206284

    Enlarge

  7. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

    Enlarge

  14. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid
    #174007
    Purpose
    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.
    Depositor
    Mark Histed
    Article
    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22
    Has Service
    AAV9
    Insert
    jGCaMP8s-P2A-ChrimsonR-ST
    Use
    AAV
    Tags
    6xHis and soma targeting motif
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    174007-plasmid-map-sequence-id-361587

    Enlarge

  15. mEGFP-C1

    Plasmid
    #54759
    Purpose
    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54759-plasmid-map-sequence-id-320190

    Enlarge

  16. pCDB179

    Plasmid
    #91960
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Christopher Bahl
    Type
    Empty backbone
    Use
    Tags
    10-His and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    91960-plasmid-map-sequence-id-221552

    Enlarge

  19. pNL [NlucP/ISRE/Hygro] Vector

    Plasmid
    #236834
    Purpose
    Express ISRE response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ISRE
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236834-plasmid-map-sequence-id-471835

    Enlarge

  23. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

    Enlarge

  28. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Promoter
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    108665-plasmid-map-sequence-id-260875

    Enlarge

  35. CMV-Hylight

    Plasmid
    #193447
    Purpose
    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Hylight Biosensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193447-plasmid-map-sequence-id-378450

    Enlarge

  37. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid
    #108542
    Purpose
    Lentivirus vector expressing Luciferase with blasticidin resistance
    Depositor
    Javier Alcudia
    Inserts
    Luciferase
    Neomycin
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1apha and PGK
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108542-plasmid-map-sequence-id-208671

    Enlarge

  38. pT18mobsacB

    Plasmid
    #72648
    Purpose
    sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72648-plasmid-map-sequence-id-399780

    Enlarge

  44. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11518-plasmid-map-sequence-id-181065

    Enlarge

  46. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Has Service
    AAV1
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

    Enlarge

  47. Artichoke

    Plasmid
    #73320
    Purpose
    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and EF1a
    Available Since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    73320-plasmid-map-sequence-id-135028

    Enlarge

  49. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Use
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

    Enlarge

Showing: 2701 - 2750 of 163777 results