8,827 results

1. pHAGE NFkB-TA-LUC-UBC-GFP-W

   Plasmid

   #49343

   Purpose

   pHAGE NFkB-TA-LUC-UBC-GFP-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   eGFP (eGFP )
   Luciferase
   NFkB consensus binding sequence
   TA
   UBC
   

   Use

   Lentiviral

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-His-hUbi

   Plasmid

   #31815

   Depositor

   Astar Winoto

   Article

   Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

   Insert

   Ubiquitin (UBB Human)
   

   Tags

   HIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVpuro-CMV-N-EGFP

   Plasmid

   #122848

   Purpose

   N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

   Plasmid

   #127289

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

   Inserts

   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and Ascl1 linked via 2A

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-mCherry

   Plasmid

   #50462

   Purpose

   Double floxed mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Promoter

   EF1a

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Rab7A

   Plasmid

   #61803

   Purpose

   Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2x HA and GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry- SEC61B

    Plasmid

    #121160

    Purpose

    Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B )
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. attB-puro donor.dna

    Plasmid

    #181923

    Purpose

    attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. P4-PE

    Plasmid

    #211375

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-PE
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-pAG-Hia5

    Plasmid

    #174373

    Purpose

    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein AG - hia5
    

    Tags

    6xHis and Protein A/G

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSNAPf-H2B Control Plasmid

    Plasmid

    #101124

    Purpose

    Expression of SNAP-tagged Histone H2B in mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. tetO.Sox9.Puro

    Plasmid

    #117269

    Purpose

    Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A and puromycin resistant cassette (C terminal …

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHREAC

    Plasmid

    #134908

    Purpose

    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    double 35S

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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