8,835 results

1. pXPR_120

   Plasmid

   #96917

   Purpose

   for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

   Depositor

   John Doench, David Root

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD33.1

   Plasmid

   #36267

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS, No promoter, and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Halo-KDEL

   Plasmid

   #124316

   Purpose

   Expresses ER specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Tags

   KDEL Seq

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST53-LRRK2-WT

   Plasmid

   #25044

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMF230

   Plasmid

   #62546

   Purpose

   Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

   Depositor

   Michael Franklin

   Article

   Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

   Insert

   GFP mut2
   

   Use

   Broad host range plasmid

   Promoter

   trc

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. gag/pol

   Plasmid

   #14887

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   gag / pol
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-GRAB-gDA3m

    Plasmid

    #208698

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Has Service

    AAV1

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUGW

    Plasmid

    #14883

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-GFP-WRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC5-3XHA-EGFP-PEX26

    Plasmid

    #139054

    Purpose

    HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

    Depositor

    David Sabatini

    Article

    Ray et al iScience. 2020 May 22;23(5):101109. doi:

    Insert

    3XHA-EGFP-PEX26
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    UBC

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GRAB_ACh3.0

    Plasmid

    #121922

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1 CMYC

    Plasmid

    #164145

    Purpose

    Doxycycline inducible expression of c-MYC

    Depositor

    Richard Possemato

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Doxycycline inducible promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. ERM-APEX2

    Plasmid

    #79055

    Purpose

    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2 anchored to the cytosolic face of the ER membrane
    

    Use

    Lentiviral

    Tags

    Residues 1-27 of P450 oxidase 2C1 and V5

    Expression

    Bacterial and Mammalian

    Promoter

    CMV immearly promotor

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSRK2706

    Plasmid

    #78571

    Purpose

    Expresses untagged human rhinovirus 3C protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    human rhinovirus 3C protease (HRVBgp1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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