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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Publication

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pCMV-GFP

Plasmid

#11153

Purpose

Mammalian expression vector for expression of GFP (CMV promoter)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has service

Cloning Grade DNA

Insert

CMV promoter-EGFP

Tags

EGFP

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

86 Articles

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Publication

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Publication

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

230 Articles

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Kevin Brindle, Duncan Jodrell

Publication

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1 and PGK

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Publication

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

67 Articles

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

142 Articles

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E-cadherin-GFP

Plasmid

#28009

Depositor

Jennifer Stow

Publication

Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

Insert

E-cadherin (CDH1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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3XFlag-pA-Tn5-Fl

Plasmid

#124601

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Steven Henikoff

Publication

Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

Insert

Protein A and hyperactive Tn5 transposase (Tnp) fusion protein

Tags

Protein A and Tn5 transposase fusion protein with…

Expression

Bacterial

Promoter

T7

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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Lamp1-RFP

Plasmid

#1817

Purpose

Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

Depositor

Walther Mothes

Publication

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

lysosome associated membrane protein 1 (Lamp1 Rat)

Tags

RFP

Expression

Mammalian

Mutation

D50E (not important for function of plasmid)

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

98 Articles

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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Publication

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

85 Articles

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pMRX-IP-GFP-LC3-RFP-LC3ΔG

Plasmid

#84572

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

69 Articles

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pLKO.3G

Plasmid

#14748

Depositor

Christophe Benoist, Diane Mathis

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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pEGFP-N1-TFEB

Plasmid

#38119

Purpose

Mammalian expression of Transcription factor EB fused to EGFP

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

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pAKTaq

Plasmid

#25712

Purpose

Bacterial expression of Taq polymerase

Depositor

David Engelke

Publication

Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

Insert

Taq polymerase

Expression

Bacterial

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCas9_GFP

Plasmid

#44719

Purpose

Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Publication

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

182 Articles

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