9,048 results

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pTet-O-Ngn2-puro

Plasmid

#52047

Purpose

3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Article

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Ngn2 (Neurog2 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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tau/pET29b

Plasmid

#16316

Depositor

Peter Klein

Article

Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Insert

tau (MAPT Human)

Expression

Bacterial

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-Bak

Plasmid

#32564

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHREAC

Plasmid

#134908

Purpose

Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

double 35S

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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3XFlag-pA-Tn5-Fl

Plasmid

#124601

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Steven Henikoff

Article

Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

Insert

Protein A and hyperactive Tn5 transposase (Tnp) fusion protein

Tags

Protein A and Tn5 transposase fusion protein with…

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-NPM WT

Plasmid

#17578

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

EGFP and Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-RACK1

Plasmid

#41088

Depositor

Anna Huttenlocher

Article

Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Insert

Receptor for activated C kinase (RACK1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-PLE-IRES-mCherry

Plasmid

#45766

Depositor

Luke Lavis

Article

Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

Insert

esterase

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP

Plasmid

#21037

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Tags

mEPFP

Expression

Bacterial and Mammalian

Available Since

May 12, 2009

Availability

Academic Institutions and Nonprofits only

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pET29b-10xHis-Super TEV

Plasmid

#193833

Purpose

Expresses high-efficiency TEV protease in bacterial cells

Depositor

EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

Article

Lau et al protocols.io, doi: 10.17504/protocols.io

Insert

TEV protease

Tags

6xHis

Expression

Bacterial

Mutation

T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAG/MNase

Plasmid

#123461

Purpose

Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

Depositor

Steven Henikoff

Article

Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

Insert

Protein A-Protein G-Micrococcal Nuclease

Tags

6xHIS and HA

Expression

Bacterial

Promoter

Lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDRGFP

Plasmid

#26475

Purpose

In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

Insert

DRGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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