8,870 results

1. pXAT2

   Plasmid

   #80494

   Purpose

   AAVS1 sgRNA expression vector

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 sgRNA-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-AAV.CAP-B10

   Plasmid

   #175004

   Purpose

   non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

   Depositor

   Viviana Gradinaru

   Article

   Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

   Insert

   Synthetic construct isolate AAV.CAP-B10 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   7 amino acid substitution between VP1 452 and VP1…

   Promoter

   p41

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1-FOXM1b

   Plasmid

   #68811

   Purpose

   Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

   Depositor

   Adam Karpf

   Article

   Barger et al Oncotarget. 2015 Jul 16.

   Insert

   FOXM1b (FOXM1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRE112

   Plasmid

   #43828

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Expression

   Bacterial

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-Flag-BRD4-WT

   Plasmid

   #90331

   Purpose

   expresses Wild type BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ai9

   Plasmid

   #22799

   Purpose

   Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed tdTomato
   

   Use

   Mouse Targeting

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKMyc

    Plasmid

    #19400

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Type

    Empty backbone

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEmerald-TOMM20-N-10

    Plasmid

    #54282

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA 3.3 LRP5

    Plasmid

    #115907

    Purpose

    Expresses human LRP5 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-FUSN-mCh-Cry2WT

    Plasmid

    #101223

    Purpose

    FUS(1-214) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-PE2max-BSD

    Plasmid

    #191102

    Purpose

    Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    PEmax-P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-FOXA2

    Plasmid

    #153110

    Purpose

    Expression of Flag tagged human FOXA2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXA2 (FOXA2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHN924

    Plasmid

    #178885

    Purpose

    Expression of GST-His8-HRV3C

    Depositor

    Kazuhiro Abe

    Insert

    human rhinovirus 3C protease
    

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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