9,111 results

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

10xHis and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3 Basic Vector

Plasmid

#212936

Purpose

Firefly luciferase vector for investigating regions controlling transcription

Depositor

Debrya Groskreutz

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#71236

Purpose

Express sgRNA and dCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A Puro
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSNAP-tag (m) Vector

Plasmid

#101135

Purpose

Expression of protein of interest with SNAP-Tag in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

SNAP-tag (SNAP26m)

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRNA-Control

Plasmid

#98398

Purpose

Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

non-targeting randomized sequence

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eB

Plasmid

#103005

Purpose

non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.eB VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB-MeCP2

Plasmid

#110821

Purpose

Improved dCas9 repressor-dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-neo

Plasmid

#21916

Purpose

3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

Depositor

Dmitri Wiederschain

Article

Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3h

Plasmid

#208699

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6b

Plasmid

#207852

Purpose

Mammalian expression of PE6b prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6b

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63-mScarlet

Plasmid

#172118

Purpose

Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

mScarlet and pHluorin_M153R in a small extracellu…

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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E-cadherin-GFP

Plasmid

#28009

Depositor

Jennifer Stow

Article

Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

Insert

E-cadherin (CDH1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Article

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_050

Plasmid

#96925

Purpose

lentiviral expression of gRNA scaffold

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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