8,873 results

1. pDONR223-PDGFRB

   Plasmid

   #23893

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti PGK GFP Puro (w509-5)

   Plasmid

   #19070

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOUP

   Plasmid

   #165419

   Purpose

   The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

   Insert

   Rep A
   

   Use

   Helper plasmid required for replication for all p…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP- SEC61B

   Plasmid

   #121159

   Purpose

   Mammalian expression of GFP tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMKO.1 GFP

   Plasmid

   #10676

   Purpose

   Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV GFP Hygro (656-4)

   Plasmid

   #17446

   Purpose

   3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Lentiviral Prep

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 mCherry

   Plasmid

   #128073

   Purpose

   puro exchanged to mCherry

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   no

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. EZ-Tet-pLKO-Hygro

    Plasmid

    #85972

    Purpose

    Tet/Dox inducible shRNA lentivirus with hygromycin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Promoter

    H1 / TetO

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV_mNG3K(1-10)

    Plasmid

    #157993

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G27D, D42E, M128I, K153M, T170I

    Promoter

    SFFV

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Su9-EGFP

    Plasmid

    #23214

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Subunit 9 of the F0-ATPase
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains AA 1-69 of subunit 9

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJC5-LAMP1-RFP-3xHA

    Plasmid

    #102932

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    RFP-HA

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Pam-FL

    Plasmid

    #42570

    Depositor

    Vijaya Ramesh

    Article

    Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

    Insert

    human MYCBP2 (MYCBP2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Pam-FL clone lacks alternatively spliced exons 52…

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Tags

    AGIA-tag

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFPGUSPlus

    Plasmid

    #64401

    Purpose

    binary plant vector expressing GFP and GUS, both driven by a 35S promoter

    Depositor

    Claudia Vickers

    Article

    Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

    Inserts

    GFP
    GUSPlus
    

    Use

    Binary vector for plant transformation

    Tags

    His

    Expression

    Plant

    Mutation

    S65T

    Promoter

    35S

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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