We narrowed to 18,622 results for: Met

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pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

Plasmid

#79372

Purpose

Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GoldenGate-VP64

Plasmid

#47389

Purpose

Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

FLAG, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Tags

myc tag

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-citrate

Plasmid

#225251

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-citrate

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKMV-VioD

Plasmid

#65350

Purpose

systhesis of vioD gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioD

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46612

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-335-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
mMaroon-PAK-mMaroon

Plasmid

#84361

Purpose

FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon-PAK-mMaroon

Tags

mMaroon flanking PAK domain on N and C terminus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMBA328

Plasmid

#214748

Purpose

Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Expression

Bacterial

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKMV-VioB

Plasmid

#65348

Purpose

systhesis of vioB gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioB

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-Ecadherin

Plasmid

#65291

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA P2

Plasmid

#80944

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-92a-Decoy.hPGK.GFP.WPRE

Plasmid

#46638

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-92a Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCytERM_mScarlet-H_N1

Plasmid

#85067

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

Plasmid

#31477

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 T2A mTagBFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y + S111Y on I-AniI = Y2 "reo" (res…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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mMaroon-RTK-mMaroon

Plasmid

#84359

Purpose

FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon-Rtk--mMaroon

Tags

mMaroon flanking Rtk domain on N and C terminus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-let7 sponge

Plasmid

#35664

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 let-7sponge

Promoter

U6

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDS277

Plasmid

#34973

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V529N

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMK401 (Hygro-P2A-mAID-Nluc)

Plasmid

#121196

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-Nluc

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12aD156R

Plasmid

#164659

Purpose

Bacterial expression of mutated LbCas12aD156R using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a-D156R

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Mutation

changed Aspartic Acid 156 to Arginine

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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SBP-EGFP-Ecadherin_puromycin

Plasmid

#65292

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

Plasmid

#81133

Purpose

mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
VAV2 DH/PH/C1 (VAV2 Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTE103

Plasmid

#59398

Purpose

MethyloBrick expression vector, fumC promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PfumC

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ1201

Plasmid

#44483

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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M-SPn-VP64

Plasmid

#48674

Purpose

Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pmScarlet-i_NES_C1

Plasmid

#85062

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mRuby2-MyosinIIA-C-18

Plasmid

#55906

Purpose

Localization: Cytoskeleton, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MyosinIIA

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGH156_MS2-AID-Hygro

Plasmid

#85415

Purpose

lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE104

Plasmid

#59399

Purpose

MethyloBrick expression vector, coxB promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PcoxB

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1272

Plasmid

#44486

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

ECFP and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pMK375 (Hygro-P2A-Stag-3FLAG)

Plasmid

#121186

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-Stag-3FLAG

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2 3xmiR-122T

Plasmid

#35672

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

Luciferase

Promoter

HSV-TK

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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UMG-SP-1_pET29b

Plasmid

#215433

Purpose

Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
UMG-SP-2_pET29b

Plasmid

#215434

Purpose

Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-C4-mCherry

Plasmid

#66530

Purpose

Expresses mCherry under the control of Mutant T7 Promoter C4

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - C4

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-TagRFP

Plasmid

#103997

Purpose

Plasmid encoding TagRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU1/Sm/3' Box_(GLuc)_TOP1

Plasmid

#68437

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U1

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mIFP24-C1

Plasmid

#54820

Purpose

Localization: C1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Tags

mIFP24

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-H_peroxisome_C1

Plasmid

#85064

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Vinculin-23

Plasmid

#58030

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vinculin (VCL Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
AAV-CamK2-tTA

Plasmid

#210731

Purpose

AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

Depositor

Wei Xu

Article

Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

tetracycline-controlled transactivator (tTA)

Use

AAV

Promoter

CamK2

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
e-ciCas9_pcDNA5

Plasmid

#100553

Purpose

Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

e-ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Clover-Rab5a-7

Plasmid

#56530

Purpose

Localization: Ras GTPase, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Rab5a

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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We narrowed to 18,622 results for: Met