8,677 results

1. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP(N3)-PP1beta

   Plasmid

   #44223

   Depositor

   Laura Trinkle-Mulcahy, Angus Lamond

   Article

   Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

   Insert

   PP1B (PPP1CB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCAR_Cas9-blast_GFP

   Plasmid

   #162074

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-blasticidin S deaminase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCBC-DT2T3

   Plasmid

   #50591

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, U6-29t plus, U6-1p, T3
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-TurboID-3MYC-natMX6 (pFB1434)

   Plasmid

   #126050

   Purpose

   For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

   Depositor

   François Bachand

   Article

   Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

   Insert

   TurboID
   

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLifeAct-mScarlet3_N1

   Plasmid

   #189767

   Purpose

   Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LifeAct-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-myc3-bTrCP

   Plasmid

   #20718

   Depositor

   Yue Xiong

   Article

   Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

   Insert

   beta-transducin repeat containing (BTRC) (Btrc Mouse)
   

   Use

   Tags

   Myc3

   Expression

   Mammalian

   Mutation

   *

   Promoter

   Available since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T4 Lysozyme WT*

   Plasmid

   #18111

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No cysteines: C54T, C97A

   Promoter

   Available since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-VP2SA11

   Plasmid

   #89163

   Purpose

   Express Rotavirus SA11 VP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Klotho (membrane)

    Plasmid

    #17712

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    P101S

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CLuc-FKBP

    Plasmid

    #31184

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Tet1

    Plasmid

    #60938

    Purpose

    Expresses Tet1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-VP1SA11

    Plasmid

    #89162

    Purpose

    Express Rotavirus SA11 VP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G Bidirectional

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLOVE-Klf4

    Plasmid

    #15950

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

    Insert

    Kruppel-like factor 4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DRH337: AAV-hsyn-CheRiff-eGFP

    Plasmid

    #51697

    Purpose

    AAV production of CheRiff channelrhodopsin actuator

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    CheRiff-eGFP
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-NSP4SA11

    Plasmid

    #89171

    Purpose

    Express Rotavirus SA11 NSP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Arf1-GFP

    Plasmid

    #39554

    Depositor

    Paul Melancon

    Article

    Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

    Insert

    ADP ribosylation factor 1 (ARF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBad-sfGFP 150TAG

    Plasmid

    #85483

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150TAG (amino acid 150 mutated to stop codon)

    Promoter

    Arabinose

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

    Plasmid

    #29712

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

    Plasmid

    #141374

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pCMV-Caspase1-flag

    Plasmid

    #21142

    Depositor

    Junying Yuan

    Article

    Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

    Insert

    caspase-1 (Casp1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBb(RSF1010)1k-GFPuv

    Plasmid

    #106395

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG425GPD-ccdB

    Plasmid

    #14154

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3/Myc-DNMT3B1

    Plasmid

    #35522

    Purpose

    Mammalian expression of human DNMT3B1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B1 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmax pona 12xTRICK 24xMS2SL

    Plasmid

    #64542

    Purpose

    TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Use

    Tags

    24xMS2 stem loops, HA, NLS, and dNP799

    Expression

    Mammalian

    Mutation

    Promoter

    ponA

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG:H2B-EGFP

    Plasmid

    #32599

    Depositor

    Anna-Katerina Hadjantonakis, Virginia Papaioannou

    Article

    Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

    Insert

    H2B-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pT7-VP4SA11

    Plasmid

    #89165

    Purpose

    Express Rotavirus SA11 VP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pT7-VP3SA11

    Plasmid

    #89164

    Purpose

    Express Rotavirus SA11 VP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSADdeltaG-mCherry

    Plasmid

    #32636

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    mCherry
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mCherry-Centrin2-N-10

    Plasmid

    #55018

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Centrin2 (CETN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT7-NSP3SA11

    Plasmid

    #89170

    Purpose

    Express Rotavirus SA11 NSP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-RNF4/pRK5

    Plasmid

    #59743

    Purpose

    Expresses RNF4 in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-EZH2

    Plasmid

    #28060

    Depositor

    Mien-Chie Hung

    Article

    Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Use

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only