4,203 results

Showing: 2251 - 2280 of 4203 results

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pcDNA3.1-Myc-14-3-3ζ

Plasmid

#48798

Purpose

expresses human myc-14-3-3ζ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAZ (YWHAZ Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pmScarlet-i_C1

Plasmid

#85044

Purpose

Mammalian expression of mScarlet-i

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry

Plasmid

#50477

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry (CHRM4 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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TRX1 flag

Plasmid

#21283

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

Flag

Expression

Mammalian

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only

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YFP-CL1

Plasmid

#11950

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

YFP-CL1

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TCF1

Plasmid

#40620

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

TCF7 (TCF7 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Dendra2-Lifeact-7

Plasmid

#54694

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

Lifeact-Dendra2

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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ER-mRFP

Plasmid

#62236

Purpose

Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

Depositor

Erik Snapp

Article

Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

Insert

ER-mRFP

Tags

mRFP1

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR7-YFP

Plasmid

#13022

Depositor

Doug Golenbock

Insert

TLR7 (TLR7 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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hACE2

Plasmid

#1786

Purpose

Mammalian expression of human ACE2

Depositor

Hyeryun Choe

Article

Li et al Nature. 2003 Nov 27. 426(6965):450-4.

Has Service

Cloning Grade DNA

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Article

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16A

Plasmid

#36155

Purpose

Expresses Sec16A as a GFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CaMPARI V398D

Plasmid

#60786

Purpose

Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI V398D

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

V398D

Promoter

CMV

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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EBFP2-C1

Plasmid

#54665

Purpose

Localization: C1 Cloning Vector, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Type

Empty backbone

Tags

EBFP2

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSIH1-puro-STAT3 shRNA

Plasmid

#26596

Depositor

Frank Sinicrope

Article

Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

Insert

STAT3 shRNA (STAT3 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only

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a1Ha-pcDNA3

Plasmid

#45809

Depositor

Edward Perez-Reyes

Article

Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

Insert

a1Ha (CACNA1H Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERP2

Plasmid

#80477

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 S HexaPro

Plasmid

#154754

Purpose

Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

Depositor

Jason McLellan

Article

Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

Insert

Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)

Tags

2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

Expression

Mammalian

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-O-FUW-Myt1l

Plasmid

#27152

Depositor

Marius Wernig

Article

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Myt1l (Myt1l Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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