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Showing: 321 - 360 of 4059 results

  6. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  14. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22036-plasmid-map-sequence-id-339901

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  15. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105530-plasmid-map-sequence-id-454420

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  20. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

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  24. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Promoter
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

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  31. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

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  38. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112202-plasmid-map-sequence-id-334619

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  39. pAAV2/8

    Plasmid
    #112864
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap8
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112864-plasmid-map-sequence-id-333531

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Showing: 321 - 360 of 4059 results