166,201 results

1. pEGFPC1-human APPL1

   Plasmid

   #22198

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. tol2-mpeg1-mcherry

   Plasmid

   #58935

   Purpose

   labels macrophages with mcherry

   Depositor

   Anna Huttenlocher

   Insert

   mpeg1-mcherry
   

   Promoter

   mpeg1

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSK-mU6-Cj-sgRNA-SV40-puro

   Plasmid

   #192128

   Purpose

   Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Cj-SgRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF5B-FRT-GFP-αTAT1

   Plasmid

   #27099

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
   

   Tags

   GFP, S-tag, and TEV site

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide-10X-CS1-3'-puro

   Plasmid

   #212011

   Purpose

   Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

   Depositor

   Danwei Huangfu

   Insert

   gRNA scaffold
   

   Use

   Lentiviral

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b-Caspase-3

   Plasmid

   #90087

   Purpose

   Expresses wild type caspase 3 in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Wild type

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCaRNA1&2

   Plasmid

   #86087

   Purpose

   Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA1, RNA2
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Tags

    mStayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCre

    Plasmid

    #236212

    Purpose

    Arabinose-inducible Cre recombinase

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    Cre recombinase
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. hRad51(A89E)–Cas9(D10A)

    Plasmid

    #125563

    Purpose

    Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(A89E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV delta R8.2

    Plasmid

    #12263

    Purpose

    First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

    Depositor

    Didier Trono

    Insert

    HIV-1 GAG/POL, Tat and Rev
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT4-CMV-GFP

    Plasmid

    #117046

    Purpose

    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

    Depositor

    Wolfgang Uckert

    Insert

    GFP
    

    Use

    Transposon

    Promoter

    CMV

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. psiCHECK2-let-7 MT

    Plasmid

    #78261

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated let-7 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CTCC to GA…

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-MLS-KillerRed

    Plasmid

    #115517

    Purpose

    Middle entry plasmid containing membrane bound KillerRed fluorophore

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-PGK-Hygro-PIP-FUCCI

    Plasmid

    #138714

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB4695

    Plasmid

    #171127

    Purpose

    A plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    OsTIR1-F74A
    

    Use

    Other

    Mutation

    non applicable

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCXLE-SOX2-17-p2a-KLF4

    Plasmid

    #193290

    Purpose

    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLJM60 FLAG-RAB35 Q67L

    Plasmid

    #107105

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. Lamp1_mStayGold

    Plasmid

    #221902

    Purpose

    Expresses mStayGold fused to Lamp1

    Depositor

    Janelia Integrative Imaging

    Insert

    Lamp1_mStayGold
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. p3E_BAG3wt-eGFP

    Plasmid

    #98180

    Purpose

    Codes for full length, GFP tagged, human wildtype Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3wt-eGFP (BAG3 Human)
    

    Use

    Gateway vector

    Tags

    GFP

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. MSCV-MYC-t2A-BCL2

    Plasmid

    #135306

    Purpose

    Overexpression of multiple human genes (BCL2 and MYC)

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    MYC (MYC Human)
    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDH-EWSR1::WT1(+KTS)

    Plasmid

    #220566

    Purpose

    Lentiviral expression of human EWSR1::WT1(+KTS)

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    EWSR1::WT1(+KTS) (WT1, EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. OPRM1-Tango

    Plasmid

    #66464

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    OPRM1 (OPRM1 Human)
    

    Tags

    FLAG and tTA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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31. pRK-flag-GFP11

    Plasmid

    #78590

    Purpose

    Expression flag-GFP 11 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 11
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

32. GFP-TIS11B

    Plasmid

    #121157

    Purpose

    Mammalian expression of GFP tagged TIS11B

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    ZFP36L1 (TIS11B) (ZFP36L1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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33. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hPGK

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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34. pCAG-Voltron

    Plasmid

    #119033

    Purpose

    Express Voltron in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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35. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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36. pcDNA3-endo-EKAR4

    Plasmid

    #205513

    Purpose

    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-EKAR4
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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37. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid

    #169247

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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38. pLV-Puro_hCOL4A4

    Plasmid

    #198099

    Purpose

    lentiviral expression of hCOL4A4 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A4 (COL4A4 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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39. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid

    #108542

    Purpose

    Lentivirus vector expressing Luciferase with blasticidin resistance

    Depositor

    Javier Alcudia

    Inserts

    Luciferase
    Neomycin
    

    Use

    Lentiviral

    Promoter

    EF1apha and PGK

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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40. pMGIB-3xFLAG-dyskerin

    Plasmid

    #53607

    Purpose

    Retroviral expression of human dyskerin

    Depositor

    Steven Artandi

    Article

    Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

    Insert

    dyskerin (DKC1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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