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168,105 results

Showing: 3681 - 3720 of 168105 results

  1. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  6. pMS2M-PH-gagpol-D64V

    Plasmid
    #166031
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    MS2-Gag-Pol
    Use
    Lentiviral
    Mutation
    D64V mutation within the integrase within Pol
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166031-plasmid-map-sequence-id-328290

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  11. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  13. p4919

    Plasmid
    #239357
    Purpose
    Expression of Rice Cullin, Rice SKP1, Rice Rbx1, and G418 resistance by read through transcription after integration into the tubulin locus
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice CUL1, Rice SKP1, Rice RBX1 and G418 resistance
    Tags
    CUL1, SKP1 and RBX1 are all Ty epitope tagged
    Promoter
    none
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239357-plasmid-map-sequence-id-478765

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  14. p5179

    Plasmid
    #239358
    Purpose
    Exrpession of Rice TIR1 F74->G, Rice ARF fragment and blaticidin resistance by read through transaction after integration int the PFR1 locus
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice TIR1, Rice ARF16 and blasticidin resistance
    Use
    Bacterial
    Tags
    TIR1 and ARF are both Ty-epitope tagged
    Mutation
    TIR1 F74->G. ARF16 is residues 784 to 959 only
    Promoter
    none
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239358-plasmid-map-sequence-id-478763

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  17. pMS82

    Plasmid
    #68110
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    Hyg
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Mutation
    Fragment
    Available Since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68110-plasmid-map-sequence-id-248992

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  22. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Mutation
    None
    Promoter
    Ribo-HX1
    Available Since
    April 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62544-plasmid-map-sequence-id-109759

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  26. pUltra-Plus-H2B-EGFP

    Plasmid
    #161776
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-EGFP from Addgene Plasmid #26790
    Use
    Lentiviral; Bicistronic
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    UbC and UbC promoter
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161776-plasmid-map-sequence-id-316824

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  30. pFLAG-CMV-hErk1

    Plasmid
    #49328
    Purpose
    Expresses FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    49328-plasmid-map-sequence-id-148204

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  34. pCAGGS-FuG-E

    Plasmid
    #67509
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci Methods. 2014 Apr 30;227:151-
    Insert
    Fusion Glycoprotein type E
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available Since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67509-plasmid-map-sequence-id-118281

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Showing: 3681 - 3720 of 168105 results