166,201 results

1. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209653

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ARTi

   Plasmid

   #214064

   Purpose

   Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   TurboRFP
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between RFP and GFP

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMAH-POLY-eRF1(E55D)

   Plasmid

   #157925

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

   Insert

   POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-pA-Hia5

   Plasmid

   #174372

   Purpose

   SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein A - hia5
   

   Tags

   6xHis and Protein A

   Expression

   Bacterial

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-EF1alpha-ZsGreen1_Puro

   Plasmid

   #192661

   Purpose

   3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   ZsGreen1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-MSP1D1deltaH4H5

   Plasmid

   #71716

   Purpose

   Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. phage ubc nls ha 2xmcp HALO

   Plasmid

   #64540

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   HA, HALO, and NLS

   Expression

   Mammalian

   Promoter

   human ubiquitin C promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIVM_26

   Plasmid

   #204501

   Purpose

   Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
   

   Expression

   Bacterial

   Mutation

   0

   Promoter

   AmpR

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLD-puro-Cc-PRDX5WT-VA

    Plasmid

    #98688

    Purpose

    Lentiviral expression of human PRDX5-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX551-CMV-CjCas9

    Plasmid

    #107035

    Purpose

    AAV plasmid expressing CjCas9 under control of CMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDawn

    Plasmid

    #43796

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p80-Katanin-mNeonGreen

    Plasmid

    #191762

    Purpose

    Expression of mNeonGreen fused to p80-Katanin

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    p80-Katanin-mNeonGreen (KATNB1 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCEP4-myc-HLA-A24

    Plasmid

    #135507

    Purpose

    Mammalian expression of myc-tagged HLA-A*024:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMET17p-CRU313

    Plasmid

    #131163

    Purpose

    For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Tags

    CRU

    Expression

    Yeast

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ultra CAST

    Plasmid

    #236111

    Purpose

    Encodes sfGFP BbsI dropout for cloning in guide RNA sequence, encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequence

    Depositor

    Jeffrey Barrick

    Article

    VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

    Type

    Empty backbone

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pF CAG luc puro

    Plasmid

    #67501

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-Hsp-eGFP-PGK-mCherry

    Plasmid

    #191862

    Purpose

    Heat inducible eGFP reporter with constitutive mCherry marker

    Depositor

    Yingxiao Wang

    Article

    Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

    Inserts

    eGFP
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    HSPA7 promoter and PGK promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PH-PLCd1-mNGx1

    Plasmid

    #202751

    Purpose

    expresses fluorescent protein-tagged lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    PLCD1v2(1-170):mNG2 (PLCD1 Human)
    

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 hE47

    Plasmid

    #16059

    Depositor

    Robert Benezra

    Article

    Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

    Insert

    E47 (TCF3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVET-IRES-GFP

    Plasmid

    #107139

    Purpose

    A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCKmatBC

    Plasmid

    #138587

    Purpose

    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    matB contains native GUG start codon

    Promoter

    Plac promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLAI-Env

    Plasmid

    #133996

    Purpose

    Env gene from HIV-1 LAI in pcDNA3.1 expression vector

    Depositor

    Himanshu Garg

    Article

    Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

    Insert

    Envelope Glycoprotein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF0612

    Plasmid

    #143054

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RBPJ (RBPJ Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA CUP1p-NuiHA kanMX

    Plasmid

    #131169

    Purpose

    PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Tags

    Nui-HA

    Expression

    Yeast

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP C3-Lats1

    Plasmid

    #19053

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Large tumor suppressor kinase 1, N mutant (LATS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    **

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiCRISPRv2-ATG5

    Plasmid

    #99573

    Purpose

    Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting ATG5 (ATG5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCLX-PGK-hCDK4

    Plasmid

    #114314

    Purpose

    Constitutive expression of the CDK4 protein

    Depositor

    Patrick Salmon

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7TS

    Plasmid

    #17091

    Depositor

    Paul Krieg

    Type

    Empty backbone

    Available Since

    June 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Flag-Src

    Plasmid

    #118308

    Purpose

    Expression of mouse Src

    Depositor

    Philip Stork

    Article

    Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

    Insert

    Src (Src Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pH6-MBP-TEV-BirA

    Plasmid

    #179694

    Purpose

    Expresses His6 tagged MBP-BirA fusion protein

    Depositor

    Mark Arbing

    Insert

    BirA
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mTurquoise2-MapTau

    Plasmid

    #137807

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    MapTau
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. tol2-mpx-mCherry

    Plasmid

    #29585

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    mpx-mCherry
    

    Use

    Zebrafish expression

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid

    #183532

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Mutation

    None

    Promoter

    nEF

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. single polypeptide FPX biosensor for caspase-3

    Plasmid

    #60883

    Purpose

    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge