159,238 results

1. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

   Plasmid

   #55643

   Purpose

   vCre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV; Vcre-dependent chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTC391

   Plasmid

   #91220

   Purpose

   protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a hMDH2

   Plasmid

   #204230

   Purpose

   Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH2 (MDH2 Human)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiGuide-zeo

   Plasmid

   #160091

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK793

   Plasmid

   #8827

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219V mutant
   

   Use

   Tags

   His, MBP (with TEV), and polyarginine

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability)

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-V0A

   Plasmid

   #210347

   Purpose

   Expresses ATP6V0A in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   V-ATPase V0A (ATP6V0A1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBPnlsLexA::p65Uw

   Plasmid

   #26230

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   nlsLexA::p65 (RELA Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Strep-KIFC1*-mCh

   Plasmid

   #120169

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a hMDH1v3 (+TEV)

   Plasmid

   #204229

   Purpose

   Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH1v3 (MDH1 Human)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Inserted a G after ATG to maintain kozak site

   Promoter

   Lac

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LbCpf1-2NLS

    Plasmid

    #102566

    Purpose

    bacterial expression of LbCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    LbCpf1
    

    Use

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

    Plasmid

    #83919

    Purpose

    Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    DsRed-Express2
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Expression

    Mutation

    Promoter

    hUbC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-FLAG-CaMKIIA

    Plasmid

    #186825

    Purpose

    Expresses FLAG-CaMKIIA in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2a (Camk2a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV Blast DEST (706-1)

    Plasmid

    #17451

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Xist-PA

    Plasmid

    #26760

    Depositor

    Rudolf Jaenisch

    Article

    Wutz et al Mol Cell. 2000 Apr . 5(4):695-705.

    Insert

    Xist (Xist Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS3 (CK2alpha, CK2beta)

    Plasmid

    #27092

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha (CSNK2A1 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-TBG-mem-TurboID

    Plasmid

    #149413

    Purpose

    Hepatocyte-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    TBG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HaloTag-sec61b

    Plasmid

    #186959

    Purpose

    Express sec61b connected with N-terminus HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Sec61 (SEC61B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-PE7-P2A-hMLH1dn

    Plasmid

    #222997

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Deletion of residues 754-756 in MLH1 as described…

    Promoter

    CMV

    Available since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAVS1-Neo-CAG-Flpe-ERT2

    Plasmid

    #68460

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT2-hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #225999

    Purpose

    To establish stable dCas9-KRAB knock-in line

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    humanized dCas9-KRAB T2A Puro
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. XLone-ETV2

    Plasmid

    #135763

    Purpose

    expresses human ETV2 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    TRE3G

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. VE-cad KI gRNA1

    Plasmid

    #92310

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA1 (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-Peroxisomes-2

    Plasmid

    #54520

    Purpose

    Localization: Peroxisomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Peroximal Targeting Signal 1 (PTS1) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCyPet-His

    Plasmid

    #14030

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Insert

    CyPet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPB-CAG-ECC-Biosensor

    Plasmid

    #140832

    Purpose

    Encoding the CAG promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    ECC-Biosensor
    

    Use

    Piggybac (pb) transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDEST53-LRRK2-G2019S

    Plasmid

    #25045

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Glycine 2019 mutated to Serine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKC96 pBABE H2B-mCherry

    Plasmid

    #192695

    Purpose

    Retroviral transgene expression of human H2B

    Depositor

    Iain Cheeseman

    Article

    Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

    Insert

    H2B
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. VE-cad KI gRNA2

    Plasmid

    #92311

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA2 (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-PGK-Hygro-PIP-FUCCI

    Plasmid

    #138714

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMax_GFP

    Plasmid

    #177825

    Purpose

    Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF3297

    Plasmid

    #144773

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF1A (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PRK5-FLAG-Bag6

    Plasmid

    #61836

    Purpose

    express N-terminally FLAG tagged human Bag6

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PASTE payload acceptor with attP site pDY2087

    Plasmid

    #219859

    Purpose

    Bxb1 payload vector with 5' attP

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge