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161,439 results

Showing: 3721 - 3760 of 161439 results

  3. eGFP-luciferase-C1

    Plasmid
    #163523
    Purpose
    Expresses both eGFP and firefly luciferase in mammalian cells
    Depositor
    Thomas Weber
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    eGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163523-plasmid-map-sequence-id-321576

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  5. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Use
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

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  7. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    NFAT enhancer + synthetic TATA box
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  8. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

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  11. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

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  19. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Article
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    61549-plasmid-map-sequence-id-212455

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  25. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Martin S. Taylor, Donna Romero
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Mutation
    Promoter
    pTRE bidirectional tet-on
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  26. pCS2+mNeonGreen-C Cloning Vector

    Plasmid
    #128144
    Purpose
    To express a protein of interest fused to the C-terminus of mNeonGreen
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mNeonGreen fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128144-plasmid-map-sequence-id-248605

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  27. CMV-Hylight-T152E

    Plasmid
    #193448
    Purpose
    A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Binding-Dead Hylight
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Threonine 67 (ACT) mutated to a Glutamic Acid (GA…
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193448-plasmid-map-sequence-id-378448

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  31. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185911-plasmid-map-sequence-id-367364

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  37. pDUAL SRBI (GFP)

    Plasmid
    #86979
    Purpose
    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    SCARB1 (SCARB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86979-plasmid-map-sequence-id-173289

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  38. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  39. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

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Showing: 3721 - 3760 of 161439 results