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We narrowed to 21,502 results for: SPR

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Showing: 3921 - 3960 of 21502 results

  1. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

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  5. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

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  6. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

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  7. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

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  9. pAAV hSyn GFP-Cre

    Plasmid
    #175381
    Purpose
    expresses GFP-Cre under the control of a human synapsin promoter
    Depositor
    Jason Aoto
    Insert
    GFP-Cre recombinase
    Use
    AAV
    Tags
    Cre is fused to GFP. GFP is fused to additional n…
    Promoter
    human synapsin
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175381-plasmid-map-sequence-id-348392

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  10. pAC1749

    Plasmid
    #127983
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127983-plasmid-map-sequence-id-254502

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  12. pJW1354

    Plasmid
    #69490
    Purpose
    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
    Depositor
    Jordan Ward
    Article
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    Use
    CRISPR; Template to generate cassettes for -media…
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69490-plasmid-map-sequence-id-122661

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  21. pEC1.2/EC1.1_spCas9_GFP

    Plasmid
    #161933
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161933-plasmid-map-sequence-id-320699

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  31. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  36. CMPE-ePPEplus

    Plasmid
    #205242
    Purpose
    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available Since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    205242-plasmid-map-sequence-id-408832

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  37. AICSDP-58: NUP153-mEGFP

    Plasmid
    #114407
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153
    Depositor
    Allen Institute for Cell Science
    Insert
    NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114407-plasmid-map-sequence-id-220837

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  40. CROPSeq-BFP

    Plasmid
    #196718
    Purpose
    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196718-plasmid-map-sequence-id-388068

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Showing: 3921 - 3960 of 21502 results