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20,197 results

Showing: 3921 - 3960 of 20197 results

  2. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  5. pcDNA3-IGFBP5-V5

    Plasmid
    #11608
    Depositor
    Steven Johnson
    Insert
    Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    no mutations
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  23. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  30. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Tags
    HA and Myr
    Expression
    Mammalian
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

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  31. pEGFP-N1-TrkB

    Plasmid
    #32500
    Depositor
    Rosalind Segal
    Insert
    TRKB (Ntrk2 Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    32500-plasmid-map-sequence-id-246445

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  38. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  39. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid
    #174660
    Purpose
    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    miniXon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174660-plasmid-map-sequence-id-351622

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Showing: 3921 - 3960 of 20197 results