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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,942 results for: LEA

Showing: 5051 - 5100 of 15942 results

  8. pAAV-Nd1

    Plasmid
    #191523
    Purpose
    Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector
    Depositor
    David Liu
    Article
    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:
    Insert
    v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    191523-plasmid-map-sequence-id-375376

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  13. pEPOR1CB0013

    Plasmid
    #117549
    Purpose
    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117549-plasmid-map-sequence-id-228087

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  27. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  33. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

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  38. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Article
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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  39. pOCC267

    Plasmid
    #118887
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease; HALO tag
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118887-plasmid-map-sequence-id-232249

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  42. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Promoter
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pNOC-CRISPR

    Plasmid
    #100008
    Purpose
    Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome
    Depositor
    Eva Farre
    Article
    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968
    Type
    Empty backbone
    Use
    Algae, nannochloropsis expression
    Tags
    Cas9-Nlux
    Expression
    Mutation
    Promoter
    Ribi promoter
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    100008-plasmid-map-sequence-id-201983

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  46. pOET3-N-TEV-Twin-Strep

    Plasmid
    #203323
    Purpose
    Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203323-plasmid-map-sequence-id-399465

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Showing: 5051 - 5100 of 15942 results