179,537 results
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Plasmid#136996PurposeLentiviral EGFP lipid sensor for PI(3)PDepositorInsertEGFP-2xFYVE
UseLentiviralTagsEGFPPromoterEF1 alphaAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-EGFP (AAV2 trial size)
Viral prep#50465-AAV2.TPurposeReady-to-use AAV2 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
ExpressionMammalianAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSAM_Bt
Plasmid#112497PurposeTransposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).DepositorInsertsINSeq-adapted mariner transposon
Himar1C9
Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-Axin1
Plasmid#109370PurposeTransient over-expression of human Axin1DepositorAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MAC-tag-C
Plasmid#108077PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 3 kb prom. CD274
Plasmid#107004PurposeModified Luciferase expression vectorDepositorAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-PKA-SPARK
Plasmid#106920PurposeExpress PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.DepositorInsertPKA-SPARK
ExpressionMammalianAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFGAMS-EGFP
Plasmid#99107PurposeExpresses human PFAS-EGFP fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
TagsEGFP (enhanced green fluorescent protein)ExpressionMammalianPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CMV.ffLuciferase.SV40
Plasmid#105532PurposeAAV expression of firefly luciferase from CMV promoterDepositorHas serviceAAV1 and AAV8InsertLuciferase
UseAAV and LuciferaseExpressionMammalianPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferasePromoterT7 promoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-SPI1
Plasmid#97039PurposeExpresses SPI1 in mammalian cellsDepositorAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lck-mTurquoise2
Plasmid#98822PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorInsertLck
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304 Luciferase-V5 blast
Plasmid#98580PurposeLentiviral vector for constitutive expression of Luciferase with C-terminal V5 tagDepositorInsertLuciferase
UseLentiviralTagsV5ExpressionMammalianAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-FLAG-SF2
Plasmid#99021PurposeMammalian expression of SF2DepositorAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-Rab27B
Plasmid#89447PurposeExpression of GFP-tagged human Rab27B in mammalian cellsDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP-AMPKa1_KD
Plasmid#79011PurposeFor mammalian expression of a kinase dead (K47R) version of AMPK alpha1.DepositorAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-AML1/ETO-IRES-GFP
Plasmid#60832PurposeAML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFPDepositorAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-YFP-rictor
Plasmid#73387PurposeExpresses HA and YFP tagged RictorDepositorInsertRPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
TagsHA and YFPExpressionMammalianPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas serviceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyExpressionMammalianPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18R6K-mini-Tn7T-Gm
Plasmid#65022PurposeGmR mini-Tn7 vector, R6K repliconDepositorPublicationTypeEmpty backboneAvailable sinceAug. 17, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianPromoterCBhAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-Neo
Plasmid#60509PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAMD1-4
Plasmid#61565PurposeStandardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-RNF4/pRK5
Plasmid#59743PurposeExpresses RNF4 in mammalian cellsDepositorAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nanog-2A-mCherry
Plasmid#59995PurposeInserts mCherry immediately downstream of the Nanog coding regionDepositorAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
Tags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER21-SOX4
Plasmid#51304PurposeExpression of SOX4 after doxycycline administrationDepositorInsertSRY (sex determining region Y)-box 4 (SOX4 Human)
UseLentiviralTagsn/aMutationfull length ORFAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-PIG-Cre
Plasmid#50935Purposeretroviral expression of Cre recombinaseDepositorPublicationInsertCre recombinase
UseCre/Lox and RetroviralTagsNLSExpressionMammalianAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-Chili
Plasmid#48687Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV10/3xflag-Clock
Plasmid#47334Purposefull length clock in pCMV10 with 3x flag at the N-terminusDepositorInsertclock
Tags3x FlagExpressionMammalianPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorPublicationInsertNLS-I-PpoI
TagsHAExpressionMammalianPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Luc
Plasmid#45968DepositorInsertLuciferase
ExpressionMammalianPromoterCMVAvailable sinceAug. 14, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-EGFP-Tau AP P301L
Plasmid#46906Purposeexpresses EGFP tagged Tau AP P301L in mammalian cellsDepositorInsertTau AP P301L (MAPT Human)
TagsEGFPExpressionMammalianMutationP301L AND all 14 S/P or T/P amino acid residues (…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Wnt Open Source
Plasmid kit#1000000022PurposePlasmids containing ORFs of every human Wnt ligand, including modified versions that enable epitope tagging.DepositorApplicationGene Expression and LabelingVector typeMammalian ExpressionAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
proE-cad670-Luc
Plasmid#42083DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseExpressionMammalianMutationcontains E-cadherin promoter region from -670 to …PromoterE-cadherinAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmyc-GFP-TNRC6A
Plasmid#41999DepositorAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGFAP-fLuc
Plasmid#40589DepositorInserthGFAP promoter (GFAP Human)
UseLuciferaseTagsFirefly luciferaseMutationContains human GFAP promoter fragment (-2163 to +…PromoterGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti 4.1 Ex miR200c-141
Plasmid#35534Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1-human lyn–GFP
Plasmid#35958PurposeExpresses human lyn protein codon optimized for zebrafish expression.DepositorAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECE-M2-BAIAP2 wt
Plasmid#31656DepositorAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ngn2
Plasmid#34999DepositorAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32481PurposeCre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by