We narrowed to 16,481 results for: LEA

1. pcDNA3-HA-CUL1 delta C22

   Plasmid

   #19942

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 delta C22 (CUL1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   Mutation of 22 amino acids from the C terminus

   Promoter

   Available Since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

2. 2xFKBP-mCh-KDEL

   Plasmid

   #173010

   Purpose

   FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   2xFKBP-mCh-KDEL
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. SuperClomeleon

   Plasmid

   #203724

   Purpose

   An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

   Depositor

   George Augustine

   Article

   Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

   Insert

   Cerulean linked topaz fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Cerulean, modified Topaz Fluorescent Protein with…

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEf1a OsTIR ires NEO pA T2BH

   Plasmid

   #127910

   Purpose

   Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   OsTIR
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-N3-TPC2

   Plasmid

   #80153

   Purpose

   Intracellular Ca2+ store marker

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

   Insert

   TPCN2 (TPCN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHLmMBP-11

   Plasmid

   #72349

   Purpose

   Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNAintron-CDC50A-c3xFLAG

   Plasmid

   #215702

   Purpose

   Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CDC50A (TMEM30A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCl-HA-YAP

   Plasmid

   #27007

   Depositor

   Joan Massague

   Article

   Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VRQR variant

    Plasmid

    #138562

    Purpose

    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQCXIX_sh_HOIP/RNF31_#6

    Plasmid

    #75252

    Purpose

    retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against human RNF31 (RNF31 )
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 6599-SUMO1GG

    Plasmid

    #13377

    Depositor

    Anne Dejean

    Article

    Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mature form ending with GG

    Promoter

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABEPuro-HA-GLI1

    Plasmid

    #62967

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV_NOPlight1

    Plasmid

    #195578

    Purpose

    Expresses NOPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

    Insert

    NOPLight1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tol2-CAG::Nucbow

    Plasmid

    #158992

    Purpose

    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Nucbow
    

    Use

    Tags

    All FPs are fused to H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSLS.419

    Plasmid

    #187208

    Purpose

    Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco4 ncRNA
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid

    #192438

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-Ahi1

    Plasmid

    #30494

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-ChRger1-TS-EYFP

    Plasmid

    #127243

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger1-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

    Plasmid

    #176587

    Purpose

    Bacterial expression of His14-MBP-TEV-AmaTnpB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Use

    Tags

    His14 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-VCPIP1

    Plasmid

    #85671

    Purpose

    Expression of VCPIP1 with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TUBG1

    Plasmid

    #87954

    Purpose

    expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

    Depositor

    Maria Alvarado-Kristensson

    Article

    Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    whole protein, The C-terminal is a sh-gamma-tubul…

    Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CS2-Myc-hASUN

    Plasmid

    #47955

    Purpose

    Expression of tagged-human ASUN

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2013 Jul 31.

    Insert

    Asunder (INTS13 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Sp6

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA6A-EGFR ICD (645-1186)

    Plasmid

    #42667

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ICD (645-1186) (EGFR Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

    Plasmid

    #169921

    Purpose

    DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

    Depositor

    Zhili Rong

    Article

    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

    Insert

    IRES-Puro-pEF1a-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Akt-STOPS-NES

    Plasmid

    #175008

    Purpose

    Cytosol-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

    Plasmid

    #122083

    Purpose

    Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-LaminB1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of LaminB1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SpCas9-NG-CGBE1

    Plasmid

    #198537

    Purpose

    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSOMA-NLS

    Plasmid

    #118937

    Purpose

    Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205320

    Purpose

    All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAba30 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSOMA-NES

    Plasmid

    #118939

    Purpose

    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GFP-WT (AF41)

    Plasmid

    #21367

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPPEDs

    Plasmid

    #162469

    Purpose

    protoplast test vector of pPPED

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Prime editing destination vector for transient ex…

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. myristoylated ct-GRK2

    Plasmid

    #178734

    Purpose

    N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

    Depositor

    Ege Kavalali

    Article

    Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

    Insert

    GRK2 (GRK2 Bovine)
    

    Use

    Lentiviral

    Tags

    Src 1-15

    Expression

    Mutation

    only amino acids 548-671

    Promoter

    Ubiquitin

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEZT-GST-SLFN2

    Plasmid

    #174319

    Purpose

    Expression of recombinant GST-SLFN2

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GST-SLFN2 (Slfn2 Mouse)
    

    Use

    Bacmam, baculovirus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-CaMKII-STChRger2-TS-EYFP

    Plasmid

    #129393

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLVX-EF1a-Neuromodulin-IRES-Puromycin

    Plasmid

    #134666

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Neuromodulin (GAP43 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pHS0806 His14-bdSUMO-AwaIscB

    Plasmid

    #176539

    Purpose

    Bacterial expression of His14-bdSUMO-AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Use

    CRISPR

    Tags

    14xHis-bdSUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV6-G2-ATP8-V5 (puromycin)

    Plasmid

    #86852

    Purpose

    mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Use

    Tags

    ATP5G2 (G2) mitochondrial targeting signal and V5

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-Ef1a-DIO-VMAT2-WPRE-pA

    Plasmid

    #39339

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Myc tag inserted in the first lumenal loop betwee…

    Promoter

    Available Since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. Ad-Flag-XBP1u

    Plasmid

    #63677

    Purpose

    Adenoviral vector for XBP1u expression

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

49. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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