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pLminP_Luc2P_RE6
Plasmid#90340PurposeSTAT5 - Stat5 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT5Available sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-H3.3-WT-FLAG-BFP
Plasmid#206139PurposeExpression of a FLAG tagged human histone H3.3 gene with BFPDepositorInsertH3.3 (H3-3A Human)
UseTagsFLAG-eBFPExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-I-PhoCl-HCVp
Plasmid#87697PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cellsDepositorInsertI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
UseTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH21-BNA-Luc
Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
UseTagsExpressionMammalianMutationPromoterRNA polymease I PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TrkB
Plasmid#32500DepositorInsertTRKB (Ntrk2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mCherry-4A/4B-CAAX
Plasmid#87699PurposeExpress mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cellsDepositorInsertmCherry-4A/4B-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
EasyClone-MarkerFree Vector Set
Plasmid Kit#1000000098PurposeYeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9DepositorAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV9)
Viral Prep#104491-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
UseTagsExpressionBacterial and YeastMutationE366G (See depositor comment below)PromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
UseTagsExpressionMammalianMutationTol2 codon usage frequency well suitable for muri…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterGPDAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV5)
Viral Prep#100845-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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RNA-binding protein pooled CRISPR knockout library
Pooled Library#141438PurposeCRISPR knockout library targets RBPs with 10 sgRNAs/geneDepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-MMLV_RT_D524N-6H
Plasmid#166945PurposeBacterial expression of M-MLV reverse transcriptaseDepositorInsertgag-pol
UseTags6X HisExpressionBacterialMutationD524N, H8YPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-GshF-blasticidin
Plasmid#210313PurposeExpresses humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptideExpressionMutationCodon optimized for expression in human cellsPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoteru6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LT3GEP
Plasmid#111172PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMutationPromoterT3GAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a dCas9-HA 2A Blast
Plasmid#102812PurposeLentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion proteinDepositorInsertdCas9-HA
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A (dCas9)PromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ÂRAGE
Plasmid#71435Purposemammalian expression of human RAGEDepositorInsertRAGE (AGER Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII0.4.eGFP.WPRE.rBG (AAV1)
Viral Prep#105541-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CamKII0.4.eGFP.WPRE.rBG (#105541). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII0.4.eGFP.WPRE.rBG plasmid DNA. Expression of EGFP from CamKII promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEGFPAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
LCV2 cGAS KO
Plasmid#217444PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human cGASDepositorInsertsgRNA targeting cGAS (CGAS Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR OVA/p2a/mCherry-CaaX
Plasmid#113030Purposefor lentivirus production of ovalbumin and mcherry-caaxDepositorInsertOVA/p2a/mCherry-CaaX
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPM1wt-IRES-mCherry
Plasmid#131824PurposeLentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transductionDepositorInsertNPM1 (NPM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-PuroR
Plasmid#174090PurposeCRISPaint gene taggingDepositorInsertmNeonGreen
UseGene taggingTagsExpressionMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Expotin 1
Plasmid#42047DepositorInsertExportin-1 (XPO1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG3
Plasmid#99577PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotypeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL093 pattB_exorh:EGFP_MCS
Plasmid#213514PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEFCas9
Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-CENPA
Plasmid#227283PurposeDonor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)DepositorInsertCENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-CENPA
Plasmid#227282PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.DepositorInsertsgRNA Targeting N-terminus of CENPA (CENPA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJLG038
Plasmid#192981PurposeBHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoterDepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-eGFP-WPRE
Plasmid#203843PurposeFlp-dependent EGFP expressionDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0235
Plasmid#142488PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralTagsExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-CMVSP6
Plasmid#138356PurposeGateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)DepositorInsertCMV-SP6 promoter
UseGateway compatibleTagsExpressionMutationPromoterCMV-SP6Available sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
Plasmid#173899PurposeThe pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThrDepositorInsertsThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PH-PLC-delta-EGFP
Plasmid#136998PurposeLentiviral EGFP lipid sensor for PI(4,5)P2DepositorInsertPH-PLC-delta-EGFP
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-Pink1
Plasmid#101874PurposeTransient expression of Pink1-YFP in mammalian cellsDepositorInsertPTEN induced putative kinase 1 (PINK1 Human)
UseTagsfluorescent tag EYFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2053
Plasmid#142912PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBCL6 (BCL6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
BK alpha subunit
Plasmid#113566Purposemouse BK channel alpha subunit expression vectorDepositorInsertKcnma1 (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKII-TRPV1-P2A-mCherry
Plasmid#200829PurposeTransduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporterDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_hsTRPA1_myc_dTom
Plasmid#183179Purposemyc tagged hsTRPA1 used for cell transfectionDepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:redCas9; control_gRNA
Plasmid#199334PurposepDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNADepositorInsertszCREST3
Cas9
control sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only
