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179,538 results

Showing: 6501 - 6550 of 179538 results

  2. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral prep
    #100849-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (#100849). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  3. pACAGW-ChR2-Venus-AAV (AAV1)

    Viral prep
    #20071-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karel Svoboda
    Publication
    Leopoldo et al Nature. 2009 Jan 18.
    Promoter
    CAGG
    Tags
    Venus
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    100902-plasmid-map-sequence-id-195323

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  9. pMaRSC

    Plasmid
    #89189
    Purpose
    The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.
    Depositor
    David Tirrell
    Publication
    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flag-L274G-T2A-mCherry (Mars1 Mouse)
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    89189-plasmid-map-sequence-id-175705

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  16. pGL4.10-VEGFprom(-1000 to -1)

    Plasmid
    #66128
    Purpose
    Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)
    Depositor
    David Mu
    Publication
    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    Use
    Luciferase
    Promoter
    VEGF -1000 to -1
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    66128-plasmid-map-sequence-id-322467

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  23. GFP-GCN5

    Plasmid
    #65386
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCN5 (KAT2A Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    65386-plasmid-map-sequence-id-159425

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  26. pCAG-VSVG

    Plasmid
    #64084
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV glycoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    64084-plasmid-map-sequence-id-107221

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  27. pSBbi-GN

    Plasmid
    #60517
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    60517-plasmid-map-sequence-id-379976

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  30. tol2-mpeg1-mcherry

    Plasmid
    #58935
    Purpose
    labels macrophages with mcherry
    Depositor
    Anna Huttenlocher
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mpeg1-mcherry
    Promoter
    mpeg1
    Available since
    Sept. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    58935-plasmid-map-sequence-id-91295

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  35. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    52421-plasmid-map-sequence-id-81252

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  39. M-ST1-sgRNA

    Plasmid
    #48672
    Purpose
    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    48672-plasmid-map-sequence-id-71846

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  48. pJMK004

    Plasmid
    #34616
    Purpose
    Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator
    Depositor
    Adam Cohen
    Publication
    Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n
    Insert
    Arch D95N
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Asparagine
    Promoter
    CaMKII
    Available since
    Jan. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    34616-plasmid-map-sequence-id-41436

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Showing: 6501 - 6550 of 179538 results