276,225 results
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Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
YAP/TAZ-TRE-mStrawberry reporter
Plasmid#158682PurposeThis plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV8)
Viral Prep#105540-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-mi3
Plasmid#159995PurposeExpresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccinationDepositorInsertSpyCatcher003-mi3
TagsC-TagExpressionBacterialPromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorMutationsee comments section belowAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
RNA-binding protein pooled CRISPR knockout library
Pooled Library#141438PurposeKnockout library targeting RNA-binding proteins with 10 guide RNAs per target.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HFā¦PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HFā¦PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCbhAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCbhAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralExpressionMammalianAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralExpressionMammalianAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
TagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-ā¦PromoterCMVAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
TagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-ā¦PromoterCMVAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
MaLionG
Plasmid#113906PurposeGreen ATP sensorDepositorInsertMaLionG
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MaLionG
Plasmid#113906PurposeGreen ATP sensorDepositorInsertMaLionG
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC035 - dLwCas13a-msfGFP
Plasmid#91925PurposeExpresses catalytically inactive LwCas13a for mammalian RNA targetingDepositorInsertdLwCas13a
UseCRISPRTagsNLS-3xHA and NLS-msfGFPExpressionMammalianMutationR488A and R1060APromoterEF-1aAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC035 - dLwCas13a-msfGFP
Plasmid#91925PurposeExpresses catalytically inactive LwCas13a for mammalian RNA targetingDepositorInsertdLwCas13a
UseCRISPRTagsNLS-3xHA and NLS-msfGFPExpressionMammalianMutationR488A and R1060APromoterEF-1aAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
ExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
ExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENN.AAV.hSyn.Cre.WPRE.hGH
Plasmid#105553PurposeAAV expression of Cre from hSyn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.WPRE.hGH
Plasmid#105553PurposeAAV expression of Cre from hSyn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
PromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
PromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Luciferase-IRES-Blast-WPRE
Plasmid#108542PurposeLentivirus vector expressing Luciferase with blasticidin resistanceDepositorInsertsLuciferase
Neomycin
UseLentiviralPromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Luciferase-IRES-Blast-WPRE
Plasmid#108542PurposeLentivirus vector expressing Luciferase with blasticidin resistanceDepositorInsertsLuciferase
Neomycin
UseLentiviralPromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-YY1
Plasmid#104395PurposeHA-YY1DepositorAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-YY1
Plasmid#104395PurposeHA-YY1DepositorAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
BL21ĪABCF
Bacterial Strain#102270PurposeompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceTetracyclineAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP
Plasmid#89684PurposepCAG promoter driving EGFPDepositorInsertEGFP
UseSynthetic BiologyExpressionMammalianAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP
Plasmid#89684PurposepCAG promoter driving EGFPDepositorInsertEGFP
UseSynthetic BiologyExpressionMammalianAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-3xFLAG-V5
Plasmid#87063PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneTags3xFLAG-V5ExpressionMammalianAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-3xFLAG-V5
Plasmid#87063PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneTags3xFLAG-V5ExpressionMammalianAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_5x Gal4 UAS
Plasmid#79119PurposeBase vector for cloning Gal4DBDVP64 inducible genesDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_5x Gal4 UAS
Plasmid#79119PurposeBase vector for cloning Gal4DBDVP64 inducible genesDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RIPK1
Plasmid#78842PurposeTo overexpress RIPK1 in Mammalian CellsDepositorAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RIPK1
Plasmid#78842PurposeTo overexpress RIPK1 in Mammalian CellsDepositorAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVax1-hVEGF165
Plasmid#74466Purposeexpression of hVEGF165 in mammalian cellsDepositorAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVax1-hVEGF165
Plasmid#74466Purposeexpression of hVEGF165 in mammalian cellsDepositorAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
Tagsyeast mitochondrial targeting sequence of citrateā¦ExpressionYeastPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
Tagsyeast mitochondrial targeting sequence of citrateā¦ExpressionYeastPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVPromoterChicken cardiac troponin TAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVPromoterChicken cardiac troponin TAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MIP 247 CoMiP 4in1 with shRNA p53
Plasmid#63726PurposeTo express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).TagsIRES-dtomatoExpressionMammalianMutationcodon-optimisedPromoterSFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
MIP 247 CoMiP 4in1 with shRNA p53
Plasmid#63726PurposeTo express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).TagsIRES-dtomatoExpressionMammalianMutationcodon-optimisedPromoterSFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pnos-Cas9-nos
Plasmid#62208PurposeExpresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoternanosAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pnos-Cas9-nos
Plasmid#62208PurposeExpresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoternanosAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI W391F V398L
Plasmid#60787PurposeExpresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI W391F V398L
TagsNuclear Export SignalExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only