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Plasmid#103852PurposeFor cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.DepositorInsertU6-RanCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHLUM (version 2)
Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_NFIC
Plasmid#187739PurposeMAC-tagged gene expression of human NFICDepositorInsertNFIC (NFIC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 Full Length-6MT
Plasmid#41728DepositorInsertNotch-1 Full Length (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationL1997V; Contains murine Notch-1 protein up to ami…PromoterAvailable sinceDec. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPD189 VP16-ZF37
Plasmid#138759PurposeConstitutive expression of VP16-ZF37 under the CMV promoter.DepositorInsertVP16-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ProRS-His
Plasmid#124117PurposeBacterial expression plasmid for PURE system componentsDepositorInsertProlyl-tRNA ligase (proS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-HisRS-His
Plasmid#124111PurposeBacterial expression plasmid for PURE system componentsDepositorInsertHistidyl-tRNA ligase (hisS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B1
Plasmid#122551PurposeBlock 1 for AdenoBuilder genome assemblyDepositorInsertAdenovirus 5 genomic region 1-3759
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TrpRS-His
Plasmid#124120PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTryptophanyl-tRNA ligase (trpS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD559 ZF173x6-Compact_EYFP
Plasmid#138873PurposeEYFP reporter vector for the ZF173x6-Compact promoterDepositorInsertZF173x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF173x6-CompactAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD539 VP16-ZF173
Plasmid#138773PurposeConstitutive expression of VP16-ZF173 under the CMV promoter.DepositorInsertVP16-ZF173
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD526 VP16-ZF13
Plasmid#138755PurposeConstitutive expression of VP16-ZF13 under the CMV promoter.DepositorInsertVP16-ZF13
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD547 ZF43x6-Compact_EYFP
Plasmid#138861PurposeEYFP reporter vector for the ZF43x6-Compact promoterDepositorInsertZF43x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-CompactAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GExpressionMutationS34C mutation in NefPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Cyclin A2
Plasmid#215048PurposeExpresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.DepositorInsertCCNA2 (CCNA2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterLTRAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PCTK3
Plasmid#20562DepositorInsertPCTK3 (CDK18 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHIV-iRFP720-E2A-Luc
Plasmid#104587PurposeLentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoterDepositorInsertiRFP720-E2A-Luciferase
UseLentiviral and LuciferaseTagsbi-cistronicExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm4b-pA
Plasmid#104486PurposeExpress mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.DepositorInsertKdm4b (Kdm4b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-zDjCas13d-His
Plasmid#234481PurposePlasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)DepositorInsertDjCas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPGK-SB13
Plasmid#236078PurposeSB13 transposase, for use with Sleeping Beauty transponson plasmidsDepositorInsertSB13 transposase
UseSleeping beauty (sb) transposase plasmidTagsExpressionMutationPromoterPGKAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC02
Plasmid#233644PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagB.DepositorInsertsfGFP with c-terminal VitelloTagB
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK005
Plasmid#233642PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagB
UseTags6-HisExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT57
Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT59
Plasmid#223431PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT03
Plasmid#223375PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GST-TEV-EGFP-ATG13
Plasmid#223797PurposeExpression of recombinant protein for purificationDepositorInsertATG13 (ATG13 Human)
UseTagsGST-TEV-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T4uvsW
Plasmid#228201PurposeExpression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealaseDepositorInsertuvsW
UseTagshexahistidine and 10 amino acid linkerExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-dRBR-HA-MND-zeo
Plasmid#232802PurposeInducible over expression of RNA-binding domain deficient form of CTCF.DepositorInsertCtcf-dRBR (Ctcf Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-WT-HA-MND-zeo
Plasmid#232801PurposeInducible over expression of wild type version of CTCF.DepositorInsertCTCF (Ctcf Mouse)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-1
Plasmid#230086PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-2
Plasmid#230087PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
2xMyc-tdMCP-SNAP (tdMCP standard)
Plasmid#226166PurposeFor transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.DepositorInsert2xMyc-tdMCP-SNAPtag
UseLentiviralTags2xMyc-tdMCP and EGFP-IRES-BlastRExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TMEM16F-I611K-peGFP-N1
Plasmid#228581PurposeExpresses mouse TMEM16F-I611K-eGFP in mammalian cellsDepositorInsertTMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
UseTagsExpressionMammalianMutationI611K plus a 3 amino acid N-terminal truncation (…PromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo_BACE1
Plasmid#227105PurposeBACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assaysDepositorInsertBACE1 (BACE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mCherry
Plasmid#223952PurposeGateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TERF1
Plasmid#222542PurposePiggyBac transposon plasmid for doxycycline inducible expression of TERF1DepositorInsertTERF1 (TERF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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TurboID-13x-EV
Plasmid#215086PurposeExpresses TurboID-13x[linker] in mammalian cells from a retroviral vector.DepositorInsertTurboID (birA )
UseRetroviralTags13x [linker]ExpressionMammalianMutationPromoterLTRAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG
Plasmid#206956PurposeAAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulinsDepositorInsertmScarlet-I, MmMT3, QtEnc
UseAAVTagsFLAGExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mPF-GFP
Plasmid#199222PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertrtTA-Advanced::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-0.5Syn-rtTA-WPRE-pA
Plasmid#183707PurposeLenti vector designed to express rtTA from a 0.5 Synapsin promoterDepositorInsertrtTA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD128 AND Gate, 3 repeats
Plasmid#138937PurposeEYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoterDepositorInsertLogic 37 AND 158 NOT 43 3 repeats
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 158 NOT 43 3 repeatsAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD543 ZF21x6-Compact_EYFP
Plasmid#138857PurposeEYFP reporter vector for the ZF21x6-Compact promoterDepositorInsertZF21x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF21x6-CompactAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD528 VP16-ZF21
Plasmid#138757PurposeConstitutive expression of VP16-ZF21 under the CMV promoter.DepositorInsertVP16-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only