Skip to main content
Addgene
Showing: 7751 - 7800 of 160228 results
  1. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA-Flag-Kdm4b-pA

    Plasmid
    #104486
    Purpose
    Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.
    Depositor
    Insert
    Kdm4b (Kdm4b Mouse)
    Use
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pAK005

    Plasmid
    #233642
    Purpose
    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.
    Depositor
    Insert
    eSpCas9(1.1) with c-terminal VitelloTagB
    Use
    Tags
    6-His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQAT59

    Plasmid
    #223431
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pYPQAT03

    Plasmid
    #223375
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Insert
    2x35s-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pYPQAT05

    Plasmid
    #223377
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Insert
    ZmUbi-SpCas9-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid
    #223952
    Purpose
    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Type
    Empty backbone
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pUt-mPF-GFP

    Plasmid
    #199222
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Insert
    rtTA-Advanced::P2A::EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pLenti-0.5Syn-rtTA-WPRE-pA

    Plasmid
    #183707
    Purpose
    Lenti vector designed to express rtTA from a 0.5 Synapsin promoter
    Depositor
    Insert
    rtTA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 7751 - 7800 of 160228 results