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173,387 results

Showing: 7751 - 7800 of 173387 results

  1. DT044A

    Plasmid
    #159405
    Purpose
    Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7/LacO promoter
    Available Since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159405-plasmid-map-sequence-id-324289

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  19. dpYPQ230-D156R-ABE8

    Plasmid
    #195569
    Purpose
    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
    Use
    CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…
    Tags
    5' and 3' NLS
    Expression
    Plant
    Promoter
    ZmUBI1
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195569-plasmid-map-sequence-id-392989

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  23. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Yan-zhen Bi
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Expression
    Mammalian
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157952-plasmid-map-sequence-id-310668

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  24. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  25. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

    Viral Prep
    #105551-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    GFP
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  31. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  35. PBX3-pSP65

    Plasmid
    #21033
    Depositor
    Corey Largman
    Insert
    PBX3 (PBX3 Human)
    Use
    In vitro tnt
    Available Since
    Aug. 14, 2009
    Availability
    Academic Institutions and Nonprofits only

  36. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  37. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

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  38. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Expression
    Bacterial
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

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  41. HaloTag-G protein gamma-2

    Plasmid
    #67018
    Purpose
    G protein gamma-2 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-2 (GNG2 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67018-plasmid-map-sequence-id-115487

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  42. pLenti.Syn(0.5).H2B-eGFP.W

    Plasmid
    #51004
    Purpose
    Lentivector that expresses H2B-eGFP from neurons-specific synapsin promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-EGFP (H2BC11 Human, Synthetic, Jellyfish)
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    rat synapsin
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51004-plasmid-map-sequence-id-130655

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  44. R777-E163 Hs.PIK3CA

    Plasmid
    #70447
    Purpose
    Gateway ORF clone of human PIK3CA [NM_006218.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CA (PIK3CA Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70447-plasmid-map-sequence-id-125896

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  45. Mito-B

    Plasmid
    #209864
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209864-plasmid-map-sequence-id-415402

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Showing: 7751 - 7800 of 173387 results