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20,441 results

Showing: 8501 - 8550 of 20441 results

  2. pOpen-DpnIR

    Plasmid
    #165529
    Purpose
    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    DpnI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165529-plasmid-map-sequence-id-339077

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  4. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Use
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87856-plasmid-map-sequence-id-175019

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  11. pBLADE(FP6**)-mCherry

    Plasmid
    #168049
    Purpose
    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.
    Depositor
    Barbara Di Ventura
    Article
    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038
    Inserts
    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101** and PBAD
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168049-plasmid-map-sequence-id-332594

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  13. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  16. pRN120

    Plasmid
    #160696
    Purpose
    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator
    Depositor
    Daniel Voytas
    Article
    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103
    Inserts
    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    Use
    Luciferase; Gemini viral replicon, plant developm…
    Tags
    Expression
    Mutation
    Promoter
    AtUbi10, CmYLCV, and Nos
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160696-plasmid-map-sequence-id-316629

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  23. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    119945-plasmid-map-sequence-id-483465

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  24. pAAV.hSynap.iAChSnFR (AAV1)

    Viral Prep
    #137950-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSynap.iAChSnFR (#137950). In addition to the viral particles, you will also receive purified pAAV.hSynap.iAChSnFR plasmid DNA. Synapsin-driven, iAChSnFR acetylcholine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Promoter
    hSynanpsin
    Tags
    None
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  26. mCherry-MyosinIIB-N-18

    Plasmid
    #55107
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pMAL-p5X

    Plasmid
    #150814
    Purpose
    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein
    Depositor
    Paul Riggs
    Type
    Empty backbone
    Use
    Tags
    malE A313V
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 22, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    150814-plasmid-map-sequence-id-306372

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  32. pCAG-B19P

    Plasmid
    #59925
    Purpose
    Expression vector for Rabies virus SAD B19 phosphoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus phosphoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59925-plasmid-map-sequence-id-94990

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  43. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  44. pTA7002-avrPto

    Plasmid
    #49156
    Purpose
    Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto
    Depositor
    Sheng Yang He
    Article
    Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100
    Insert
    avrPto
    Use
    Plant transformation binary vector
    Tags
    Expression
    Mutation
    Promoter
    dexamethasone inducible
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. HSC1-HS4-D4Z4c-GiP

    Plasmid
    #58541
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58541-plasmid-map-sequence-id-90716

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  46. pCMV-Flag-hMcl-1

    Plasmid
    #25392
    Depositor
    Roger Davis
    Insert
    MCL1 (MCL1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Academic Institutions and Nonprofits only
    25392-plasmid-map-sequence-id-316915

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Showing: 8501 - 8550 of 20441 results