Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Applied Filters

20+ requests Clear filter

20,806 results

Showing: 8551 - 8600 of 20806 results

  10. pcDNA3-Ii-OVA.I+II

    Plasmid
    #64596
    Purpose
    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation
    Depositor
    Sandra Diebold, Martin Zenke
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Tags
    Invariant chain
    Expression
    Mammalian
    Mutation
    Only amino acids 242-353 (class I and class II ep…
    Promoter
    CMV
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64596-plasmid-map-sequence-id-118017

    Enlarge

  14. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Article
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway cloning
    Tags
    SspB and mCherry
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Available Since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

    Enlarge

  15. GPR161-Tango

    Plasmid
    #66334
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR161 (GPR161 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available Since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

    Enlarge

  28. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

    Enlarge

  29. FLX1.8MitoKaede

    Plasmid
    #28133
    Depositor
    Geoffrey Owens
    Insert
    MitoKaede
    Use
    Lentiviral
    Tags
    COX VIII signal sequence
    Expression
    Mammalian
    Available Since
    Oct. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    28133-plasmid-map-sequence-id-13329

    Enlarge

  33. mCherry-PLK1-N-16

    Plasmid
    #55119
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    PLK1 (PLK1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55119-plasmid-map-sequence-id-424730

    Enlarge

  36. pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP (AAV8)

    Viral Prep
    #137153-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP (#137153). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of NpHR3.3-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre-dependent)
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  40. R777-E287 Hs.ROS1

    Plasmid
    #70571
    Purpose
    Gateway ORF clone of human ROS1 [NM_002944.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROS1 (ROS1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70571-plasmid-map-sequence-id-126020

    Enlarge

  46. pBABE

    Plasmid
    #21836
    Depositor
    David Ron
    Insert
    EGFP
    Use
    Retroviral
    Tags
    farnysylation signal
    Expression
    Mammalian
    Available Since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21836-plasmid-map-sequence-id-10029

    Enlarge

  49. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

    Enlarge

Showing: 8551 - 8600 of 20806 results