20,441 results

1. pUCsRNAP

   Plasmid

   #182746

   Purpose

   Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Insert

   small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLNCX2-mCherry-CHMP2A

   Plasmid

   #115330

   Purpose

   expresses mCherry-CHMP2A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF-Bos TRIF Flag

   Plasmid

   #41550

   Depositor

   Kate Fitzgerald, Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   Trif (TICAM1 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pLYS1-FLAG-MitoGFP-HA

   Plasmid

   #50057

   Purpose

   Expression of GFP targeted to mitochondria with Flag and HA tags

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   Mito-GFP
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-ZMYND8

   Plasmid

   #65401

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   ZMYND8 (ZMYND8 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-NSP3SA11

   Plasmid

   #89170

   Purpose

   Express Rotavirus SA11 NSP3 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn Con/Fon EYFP

   Plasmid

   #55650

   Purpose

   Cre-on/Flp-on EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   eYFP with introns
   

   Use

   AAV; Cre on/flp on eyfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM553

   Plasmid

   #68543

   Purpose

   NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

   Depositor

   Christopher Voigt, Timothy Lu

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-Cre-GFP

   Plasmid

   #68544

   Purpose

   AAV expression of Cre-GFP fusion

   Depositor

   Eric Nestler

   Article

   Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTagBFP2-TOMM20-N-10

    Plasmid

    #55328

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. G-CaMP3

    Plasmid

    #22692

    Purpose

    a single-wavelength GCaMP2-based genetically encoded calcium indicator

    Depositor

    Loren Looger

    Article

    Tian et al Nat Methods. 2009 Dec;6(12):875-81.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N363D mutation (increased fluorescence)

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBRIT Pax7 FLAG

    Plasmid

    #17521

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-S5E2-dTom-nlsdTom

    Plasmid

    #135630

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plRL2

    Plasmid

    #163631

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

    Plasmid

    #84913

    Purpose

    Mammalian expresion of TDP-43 NLS1, 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMJ826

    Plasmid

    #39316

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Histidine 840 to Alanine (H840A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX hCD4

    Plasmid

    #14614

    Depositor

    Dan Littman

    Article

    Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

    Insert

    CD4 (CD4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-tetO-myc T58A

    Plasmid

    #19763

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDIRECT_22A

    Plasmid

    #91133

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. phMYT1L-N106

    Plasmid

    #60861

    Purpose

    Expresses MYT1L under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Frt-V5-hspB8

    Plasmid

    #63109

    Purpose

    Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HIV-1 Gag-mCherry

    Plasmid

    #85390

    Purpose

    Visualization marker for viral core to measure cellular uptake

    Depositor

    Gregory Melikyan

    Article

    Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

    Insert

    HIV-1 gag
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBA439

    Plasmid

    #85967

    Purpose

    Perturb-seq vector backbone

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-3-VEGFR3

    Plasmid

    #119230

    Purpose

    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.

    Depositor

    Kari Alitalo

    Insert

    VEGFR3 (FLT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEE515

    Plasmid

    #149288

    Purpose

    T-DNA encoding TRV2 complete genome with MCS

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SIVl

    Plasmid

    #81090

    Purpose

    Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET16b-PCNA

    Plasmid

    #134898

    Purpose

    Bacterial expression and purification of human PCNA

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    fully synthetic, codon optimized for bacterial ex…

    Promoter

    T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pK18sB

    Plasmid

    #177838

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENTR.CMV

    Plasmid

    #32688

    Purpose

    Mammalian expression and gateway entry vector

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT7-VP3SA11

    Plasmid

    #89164

    Purpose

    Express Rotavirus SA11 VP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
    

    Use

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Promoter

    Available since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSBbi-GB

    Plasmid

    #60520

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-TRE-mNeonGreen

    Plasmid

    #99131

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDONR223_BRAF_p.V600E

    Plasmid

    #81700

    Purpose

    Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    V600E

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBABE-FLAG-KD LKB1

    Plasmid

    #8593

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K78I: lysine 78 mutated to isoleucine; kinase dead

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-TBG-Cyto-TurboID

    Plasmid

    #149414

    Purpose

    Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    Cyto-TurboID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mutation

    Promoter

    TBG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-CAG-mTurquoise2

    Plasmid

    #99122

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSLIK-rABE-Flag

    Plasmid

    #191383

    Purpose

    Doxycycline inducible expression of RNA A-to-I editor rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    rABE-Flag
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    tet-inducible promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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