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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 18,718 results for: IRE

Showing: 8651 - 8700 of 18718 results

  1. pDBEcR/Bla

    Plasmid
    #201556
    Purpose
    Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201556-plasmid-map-sequence-id-400918

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  9. pUpstream2Lox

    Plasmid
    #164573
    Purpose
    Integration of 2 LoxN sites in the genome of P. berghei
    Depositor
    Olivier Silvie
    Inserts
    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164573-plasmid-map-sequence-id-325424

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  10. pJH3456

    Plasmid
    #179581
    Purpose
    Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    BFP
    Expression
    Worm
    Mutation
    Promoter
    Pnmr-1
    Available since
    March 2, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    179581-plasmid-map-sequence-id-355714

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  11. pGG184

    Plasmid
    #165604
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    'CAGCG' PAM replacing the 'CGGCG&#…
    Promoter
    lac
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165604-plasmid-map-sequence-id-334628

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  16. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid
    #136646
    Purpose
    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC
    Depositor
    Karl Matter, Maria Balda
    Article
    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.
    Insert
    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    Use
    Tags
    HA (MQDLPGNDNSTAG)
    Expression
    Mammalian
    Mutation
    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136646-plasmid-map-sequence-id-271745

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  26. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid
    #185687
    Purpose
    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).
    Depositor
    Peter Walter
    Article
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Insert
    ERmembrane-GST-2xHaloTag-KKMP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185687-plasmid-map-sequence-id-366415

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  38. pENV8(GAAA)-GFPuv

    Plasmid
    #70128
    Purpose
    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
    Depositor
    Robert Batey
    Article
    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:
    Insert
    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70128-plasmid-map-sequence-id-130022

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  46. pOPS0384

    Plasmid
    #133234
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133234-plasmid-map-sequence-id-287535

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Showing: 8651 - 8700 of 18718 results