20,807 results
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pSimpleII-NLS-NmCas9-HA-NLS(s)
Plasmid#47867PurposeThis plasmid encode NmCas9 with two NLS and a HA tagDepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pToneDest
Plasmid#67691PurposeattR4-attR3 Destination cassette in Tol1 backboneDepositorTypeEmpty backboneAvailable SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionInsectPromoterPolyhedronAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-3xFLAG-NLS-SpCas9-NLS
Plasmid#51307PurposeCas9 expression plasmidDepositorInsert3xFLAG-NLS-SpCas9-NLS
UseCRISPR; Xenopus tropicalis expressionTags3x Flag and NLSPromoterCMVAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
Tags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKalpha
Plasmid#15467DepositorAvailable SinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-N
Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A3
Plasmid#135505PurposeMammalian expression of myc-tagged HLA-A*03:01DepositorInsertHLA-A*03:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKC delta WT
Plasmid#16386DepositorAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
NGFR
Plasmid#27489DepositorTypeEmpty backboneUseRetroviralTagsECMV, IRES, and NGFRExpressionMammalianAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneTagsFLAG and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-25:MAP1LC3B-mEGFP
Plasmid#101783PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3BDepositorInsertMAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-GFAP-hChR2(H134R)-mCherry
Plasmid#27055PurposeAAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitationDepositorInserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterGFAPAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Lck-GFP
Plasmid#118738PurposeTo express membrane targeted GFPDepositorAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Fos
Plasmid#131595Purposeexpression of mouse Fos in mammalian cellsDepositorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A
Plasmid#102585PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 SARS-CoV 3CLpro
Plasmid#61692Purposeinducible expression of SARS 3CLpro proteaseDepositorInsertSARS 3CLpro protease
TagsGST and HISExpressionBacterialPromotertacAvailable SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianPromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-eGFP-CCR5TC
Plasmid#135530PurposeEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
TagseGFPExpressionMammalianPromoterCAGAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB411
Plasmid#74805PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHes1-GFPd2
Plasmid#14808DepositorInsertHes1 promoter
TagsGFPd2ExpressionMammalianAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN3-pcDNA3
Plasmid#18960DepositorAvailable SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLH-nmsgRNA1.1
Plasmid#64115PurposeVector for expression of Nm sgRNA1.1 in mammalian cellsDepositorInsertNm sgRNA1.1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176272PurposeExpression of non-targeting sgRNA of CasMINIDepositorInsertCasMINI non-targeting sgRNA (sgLacZ) and BFP
UseLentiviralExpressionMammalianPromoterU6Available SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-flag-myr-Akt1-IRES-GFP
Plasmid#65063Purposemammalian retroviral expression plasmidDepositorAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_SEPT2
Plasmid#71547Purposemammalian expression of Sept 2 with a GFP fusionDepositorAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC53.2
Plasmid#26536PurposeT/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probesDepositorTypeEmpty backboneUseT/a cloning vector for dsrna generation and the g…Available SinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0000 (No LUC)
Plasmid#44465DepositorTypeEmpty backboneUsePlant transformationAvailable SinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
PKT2/CLP-AKT
Plasmid#20281DepositorInsertAKT (AKT1 Human)
UseTransposonAvailable SinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL
Plasmid#66836PurposeExpression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.DepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-CAMKIIbeta
Plasmid#21227DepositorAvailable SinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-His6
Plasmid#182386PurposeBacterial expression of Vesicle Nucleating peptideDepositorInsertVNp-His
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
VN-alpha Synuclein
Plasmid#89470Purposeexpresses human alpha Synuclein fused to the N-terminal part of VenusDepositorAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
RA-NES
Plasmid#61019PurposeddRFP A-NESDepositorInsertddRFP A
TagsHindIII site and NES sequence MNLVDLQKKLEELELDEQQExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-BetaActin-SecA5-YFP
Plasmid#105664PurposeUbiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporterDepositorAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt3
Plasmid#35909DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFC330
Plasmid#87842PurposeAMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection markerDepositorInsertsCas9
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149198)
Plasmid#80248Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 EGFP-TEADi
Plasmid#140145PurposeTetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)DepositorInsertEGFP TEADi
UseLentiviralTagsEGFPExpressionMammalianAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau AP P301L
Plasmid#46906Purposeexpresses EGFP tagged Tau AP P301L in mammalian cellsDepositorInsertTau AP P301L (MAPT Human)
TagsEGFPExpressionMammalianMutationP301L AND all 14 S/P or T/P amino acid residues (…PromoterCMVAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL2B -1538/+64
Plasmid#24942DepositorAvailable SinceAug. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLX304 ERG
Plasmid#118625PurposeOverexpresses ERG proteinDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Plk1(829)
Plasmid#39845DepositorInsertPlk1 (PLK1 Human)
ExpressionMammalianAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
hyeA3A-BE4max
Plasmid#157944PurposeMammalian expression of hyeA3A-BE4maxDepositorInserthyeA3A-BE4max
UseCRISPRExpressionMammalianMutationN57GAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 K232R
Plasmid#80878Purposemammalian expression of ALK5 K232RDepositorAvailable SinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-LKB1
Plasmid#21147DepositorAvailable SinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-STAT3-S727D
Plasmid#73364PurposeExpression of STAT3-S727DDepositorInsertSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
TagsFLAG taggedExpressionMammalianMutationE16K S727DPromoterCMVAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only
