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Plasmid#10741DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationAmino acids 1-274.PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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plIVMD164
Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_H2B
Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/intron/FlagCter
Plasmid#113548PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorInsertFlag
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mPAGFP
Plasmid#168493PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGF173
Plasmid#96999PurposeNLS-GFP-lacZ leu1 insertion vectorDepositorInsertlacZ
UseTagsNLS-GFPExpressionYeastMutationPromoterPnmt1Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Desmocollin2_tail-GST
Plasmid#36992DepositorInsertDsc2 (DSC2 Human)
UseTagsGSTExpressionBacterialMutationIntracellular domain of Dsc2 (nt 2,603–3,158)PromoterAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
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vector plix 4.03
Plasmid#121130Purposeinducible expression in cellsDepositorInsertempty cassete for contol of miR expression
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-PDZ
Plasmid#127478Purposecoding sequence for synthetic zinc finger ZF-BCB-PDZDepositorInsertZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
UseCell-free transcription–translationTagsalpha 1-syntrophin PDZ domainExpressionMutationPromoterT7Available sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDY4
Plasmid#53589PurposeProduces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.DepositorInsertlcyE
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN15-mVenus
Plasmid#168486PurposeMammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLLX8
Plasmid#79838PurposeContains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vectorDepositorTypeEmpty backboneUseContains yeast counter selection gene, cyh2 and b…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
814 pSG5L HA PTEN 1-336
Plasmid#10742DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationAmino acids 1-336.PromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMK1224
Plasmid#84221PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFPDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcihygrosynDAT
Plasmid#19992DepositorInsertsynDAT (SLC6A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-100u
Plasmid#13547DepositorInsertFLIP-E S118W
UseTagsECFP and VenusExpressionMammalianMutationS118W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET:CCCT:GFP
Plasmid#60738PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Sfxn3
Plasmid#48169Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of SFXN3 (SFXN3 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
rGSKmyc-pXT7
Plasmid#15492DepositorInsertGSK-3 beta (Gsk3b Rat)
UseXenopus rna expressionTagsmycExpressionMutationPromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pTYB3-human PUM1-HD MUT1-1 (Q867E)-intein-CBD
Plasmid#73301PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationQ867EPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU#1450_unc-53a_cDNA
Plasmid#82361Purposeexpress unc-53a cDNADepositorInsertunc-53 a isoform (unc-53 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW216J106-Amp32C
Plasmid#78651PurposepSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tTDepositorInsertJ106-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR S155A
Plasmid#72702PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationS155APromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAG416GPD-Cerulean-CHC1
Plasmid#18850DepositorInsertCHC1 (CHC1 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_LAMP1
Plasmid#65686PurposeExpresses LAMP1-ONL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 3904 (=HL 770 + pHL 991)
Plasmid#30093DepositorInsertpLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pRRG159-foxD
Plasmid#99068PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea foxDDepositorInsertfoxD in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-L
Plasmid#127477Purposecoding sequence for synthetic zinc finger ZF-ADD-LDepositorInsertZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
UseCell-free transcription–translationTagsPDZ cognate peptide ligand (VKESLV)ExpressionMutationPromoterT7Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-GCN
Plasmid#127475Purposecoding sequence for synthetic zinc finger ZF-ADD-GCNDepositorInsertZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-microRNA 146a-Dendra2
Plasmid#97113Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 146a Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_H2B
Plasmid#65704PurposeExpresses ONL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-microRNA 199-3-Dendra2
Plasmid#97119Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 199 3 Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGSTag DLC2
Plasmid#24270DepositorInsertDLC2 (Dynll2 Mouse)
UseTagsGstExpressionBacterialMutationPromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Park2_L (TAL 3012)
Plasmid#36004DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
Park2_R (TAL 3013)
Plasmid#36005DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBW214J115-Amp32C
Plasmid#78649PurposepSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ115-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
As5 (pBW414J105-arsR)
Plasmid#123162PurposeArsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-tDepositorInsertJ105-30arsR-t-ParsR-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ105, ParsRAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-FW/AA
Plasmid#12293DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y, F774A and W775A; signal peptide (aa1…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_PTS2
Plasmid#65677PurposeExpresses PTS2-ONL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 3722 (=HL 716 + pHL 404)
Plasmid#30084DepositorInsertpLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBW211J109-Amp32C
Plasmid#78646PurposepSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ109-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_H2B
Plasmid#65703PurposeExpresses CNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ptp4a3_L (TAL 3000)
Plasmid#35992DepositorInsertTAL array targeting ptp4a3 (ptp4a3a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -