We narrowed to 11,160 results for: ENA

1. pRDA_722

   Plasmid

   #216077

   Purpose

   CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Destination

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmCherry-C1_SEPT9 i1

   Plasmid

   #71622

   Purpose

   mammalian expression of human SEPT9 with an mCherry fusion

   Depositor

   Elias Spiliotis

   Article

   Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MAC_C_PTH2

   Plasmid

   #111674

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PTH2 (PTRH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA302

   Plasmid

   #216052

   Purpose

   Fragmid fragment: (C' terminus) Nucleoplasmin NLS

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   NpNLS_v1.2; Stop; polyA_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJFRC-nSyb-IVS-PhiC31

   Plasmid

   #133868

   Purpose

   Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   PhiC31 Recombinase (int )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. J23100_GB

   Plasmid

   #65983

   Purpose

   MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Constitutive promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTriEx-mCherry::LANS4

   Plasmid

   #60785

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS4
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLyGo-Kp-1

   Plasmid

   #163143

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAT007

    Plasmid

    #171634

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX4T-HEPN

    Plasmid

    #75296

    Purpose

    Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

    Insert

    Human Sacsin 4441-4579
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET-WHaloCaMP1a-EGFP

    Plasmid

    #205303

    Purpose

    Expression of WHaloCaMP1a-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET303-ColE1-T-E192C

    Plasmid

    #180233

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domains (residue…

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pREDIT_Cas9-MS2-BB_BbsI

    Plasmid

    #164802

    Purpose

    REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dmBACCS2-IRES-dOrai

    Plasmid

    #67627

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-Venus-pLenti6

    Plasmid

    #36948

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    Venus (EYFP)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    -F46L/F64L/V68L/M153T/V163A/S175G

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJMP1183

    Plasmid

    #119254

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PDGFR-TMD (MTK 3B) (pZYW025)

    Plasmid

    #194218

    Purpose

    Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PDGFR TMD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. T2A-mCherry (MTK 4a) (pAN3844)

    Plasmid

    #194223

    Purpose

    Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    T2A-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

    Plasmid

    #194229

    Purpose

    Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB1-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pR1G108CP20X

    Plasmid

    #85086

    Purpose

    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-HA-TRAIL-R1

    Plasmid

    #61382

    Purpose

    Expresses HA tagged TRAIL receptor 1

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 1
    

    Use

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R1 leader peptide (aa1-23) replaced with TN…

    Promoter

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

    Plasmid

    #83947

    Purpose

    gRNA cassette-carrying vector with natMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    gRNA targeting ADE2 gene
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSmart-EF1a-Nla-cas5

    Plasmid

    #178878

    Purpose

    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas5c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSmart-EF1a-Nla-cas8

    Plasmid

    #178880

    Purpose

    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas8c-bGH PolyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSmart-EF1a-Nla-cas7

    Plasmid

    #178879

    Purpose

    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas7c-bGH PolyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJFRC-20XUAS-IVS-PhiC31

    Plasmid

    #133565

    Purpose

    Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-(-30)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62372

    Purpose

    Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP-9xGGS-Cre-6xHis
    

    Use

    Tags

    (-30)GFP and 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FUW-TetO-Nanog

    Plasmid

    #40800

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV + TetO

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

38. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTC364

    Plasmid

    #91213

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AA011

    Plasmid

    #215973

    Purpose

    Fragmid fragment: (N' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AA010

    Plasmid

    #216038

    Purpose

    Fragmid fragment: (C' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    UGI_v1.1; NpNLS_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AA013

    Plasmid

    #215975

    Purpose

    Fragmid fragment: (N' terminus) for A>G base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-N2-RIPK3

    Plasmid

    #78822

    Purpose

    To detect localization of RIPK3 protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

44. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pDR-GFPuniv

    Plasmid

    #46085

    Purpose

    In vivo recombination assay fluorescent reporter

    Depositor

    Raymond Monnat

    Article

    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

    Insert

    DR-GFPuniv reporter
    

    Use

    Recombination reporter plasmid

    Tags

    Expression

    Mammalian

    Mutation

    E5G and K163R mutations in EGFP relative to wild-…

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLKO.1-sh-mSOX9-5

    Plasmid

    #40646

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-5 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

47. pN006

    Plasmid

    #188470

    Purpose

    Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    AsCas12a
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. PB-SRT-tdTomato

    Plasmid

    #154885

    Purpose

    piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    piggyBac SRT
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pTC394

    Plasmid

    #91224

    Purpose

    protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCas9-beta

    Plasmid

    #169240

    Purpose

    Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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