Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

Bacterial Expression

29,712 results

Showing: 2351 - 2400 of 29712 results

  3. mu-IFNg-pCIneo

    Plasmid
    #163517
    Purpose
    Expresses murine IFN gamma in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-IFN gamma (Ifng Mouse)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163517-plasmid-map-sequence-id-321578

    Enlarge

  5. PGAM5

    Plasmid
    #38976
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PGAM5 (PGAM5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pT2/HB

    Plasmid
    #26557
    Depositor
    Perry Hackett
    Insert
    pT2/HB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    26557-plasmid-map-sequence-id-352286

    Enlarge

  13. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Insert
    SARS-2 Spike Protein (S SARS-COV-2, Synthetic, Human)
    Use
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    standard CMV
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

    Enlarge

  16. p404TEF1

    Plasmid
    #15972
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15972-plasmid-map-sequence-id-8457

    Enlarge

  21. GST-PR-LBD

    Plasmid
    #89117
    Purpose
    bacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1
    Depositor
    Elizabeth Wilson
    Insert
    human progesterone receptor ligand binding domain (PGR Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains Ligand Binding Domain (637-933)
    Promoter
    tac
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89117-plasmid-map-sequence-id-175728

    Enlarge

  25. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid
    #228469
    Purpose
    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production
    Depositor
    Mary Dunlop
    Article
    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102
    Inserts
    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23106, J23108, and T7A1
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    228469-plasmid-map-sequence-id-461218

    Enlarge

  26. pBDC

    Plasmid
    #135188
    Purpose
    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    CmR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135188-plasmid-map-sequence-id-273773

    Enlarge

  27. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid
    #172506
    Purpose
    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 bacterial expression vector with golden gate site for guides
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172506-plasmid-map-sequence-id-344928

    Enlarge

  28. bNS2B47NS3

    Plasmid
    #155320
    Purpose
    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155320-plasmid-map-sequence-id-318477

    Enlarge

  31. APRT

    Plasmid
    #64028
    Purpose
    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
    Depositor
    Vern Schramm
    Article
    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9
    Insert
    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    Use
    Tags
    Histidine x 6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

    Enlarge

  33. pBLADE(FP6**)-mCherry

    Plasmid
    #168049
    Purpose
    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.
    Depositor
    Barbara Di Ventura
    Article
    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038
    Inserts
    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101** and PBAD
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168049-plasmid-map-sequence-id-332594

    Enlarge

  34. pBAD-mLemon

    Plasmid
    #191256
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein mLemon
    Depositor
    Robert Campbell
    Insert
    mLemon
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    191256-plasmid-map-sequence-id-378486

    Enlarge

  41. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

    Enlarge

  47. PARP2-c098

    Plasmid
    #216873
    Purpose
    Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion
    Depositor
    Lari Lehtiö
    Article
    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10
    Insert
    PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216873-plasmid-map-sequence-id-434366

    Enlarge

  49. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Use
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

    Enlarge

  50. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid
    #218514
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(1)
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218514-plasmid-map-sequence-id-444100

    Enlarge

Showing: 2351 - 2400 of 29712 results