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Mammalian Expression

76,388 results

Showing: 9001 - 9050 of 76388 results

  3. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87421-plasmid-map-sequence-id-441601

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  4. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55005-plasmid-map-sequence-id-456544

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  6. THINGL pcDNA3.1

    Plasmid
    #230046
    Purpose
    Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230046-plasmid-map-sequence-id-458381

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  7. pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156912
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IGSF8 (IGSF8 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156912-plasmid-map-sequence-id-444413

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  11. pcDNA3.1-GGamma4-GFP2

    Plasmid
    #166776
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma4-GFP2 (GNG4 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166776-plasmid-map-sequence-id-330999

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  13. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Use
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189755-plasmid-map-sequence-id-385774

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  19. pVITRO1-dV-IgG1/κ

    Plasmid
    #52213
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52213-plasmid-map-sequence-id-322304

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  25. OptoEphB2KD-mCherry

    Plasmid
    #92044
    Purpose
    Expresses kinase-dead mutant of OptoEphB2 labeled with mCherry
    Depositor
    Ji Yu
    Insert
    EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92044-plasmid-map-sequence-id-182373

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  26. pCI-Puro_hCOL4A2-Myc-His-WPRE

    Plasmid
    #198080
    Purpose
    Express hCOL4A2 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A2 (COL4A2 Human)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198080-plasmid-map-sequence-id-390107

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  30. pT7-EGFP-C1-HsYTHDF3_AB

    Plasmid
    #148313
    Purpose
    Mammalian Expression of HsYTHDF3
    Depositor
    Elisa Izaurralde
    Insert
    HsYTHDF3 (YTHDF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148313-plasmid-map-sequence-id-389938

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  36. PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11

    Plasmid
    #216394
    Purpose
    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)
    Depositor
    Evgeny Kvon
    Article
    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human beta-globin
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216394-plasmid-map-sequence-id-427267

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  41. TFORF2161

    Plasmid
    #141950
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL1 (GRHL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141950-plasmid-map-sequence-id-309802

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  43. pAAV-Syn-Chronos-tdTomato

    Plasmid
    #62726
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV8
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62726-plasmid-map-sequence-id-195120

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  48. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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Showing: 9001 - 9050 of 76388 results