76,397 results

1. pscAAV-CAG-GFP

   Plasmid

   #83279

   Purpose

   recombinant AAV vector packaging self-complementary GFP under the CAG promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   enhanced green fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ABE8e(TadA-8e V106W)

   Plasmid

   #138495

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e V106W)-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA-8e V106W

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiGuide-puro-NLS-mCherry

   Plasmid

   #185474

   Purpose

   Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIPZ shSTING-Hsa

   Plasmid

   #128158

   Purpose

   Doxycyclin inducible shRNA knockdown of human STING gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shSTING Hsa (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRGFP

   Plasmid

   #26475

   Purpose

   In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

   Depositor

   Maria Jasin

   Article

   Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

   Insert

   DRGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ZNF598-TEV-3xFLAG

   Plasmid

   #105690

   Purpose

   Expresses C terminally 3xFLAG tagged ZNF598 protein

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Insert

   ZNF598 (ZNF598 Human)
   

   Use

   Tags

   TEV-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-Hygro-eGFP

    Plasmid

    #99375

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PKA-KTR-mStrawberry reporter

    Plasmid

    #158688

    Purpose

    This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AttB _EGFP

    Plasmid

    #171597

    Purpose

    Strong EGFP fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    EGFP
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-BFP-sesRNA-GFP-WPRE-pA

    Plasmid

    #192063

    Purpose

    Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for tdTom
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT3-EF1A-mTert

    Plasmid

    #162555

    Purpose

    Overexpression of Tert

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of Tert (Tert Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC081: pAAV.CMV.mCherry

    Plasmid

    #205744

    Purpose

    AAV vector for expressing CMV-driven mCherry reporter gene.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1A eIF4A3

    Plasmid

    #83035

    Purpose

    mammalian expression of eIF4A3

    Depositor

    Gideon Dreyfuss

    Article

    Chan et al RNA. 2004 Feb;10(2):200-9.

    Insert

    eIF4A3 (EIF4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pCMV_AncBE4max_P2A_GFP

    Plasmid

    #112100

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMslc

    Plasmid

    #32095

    Depositor

    Christopher Buck

    Article

    Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

    Insert

    SLC35A1 (SLC35A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FerH

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Mitochondrial PancACe

    Plasmid

    #215710

    Purpose

    Expresses acetyl-CoA biosensor in the mitochondria

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and mitochondrial localization sequence…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti6-H2B-mCherry

    Plasmid

    #89766

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    Histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSBbi-Bla

    Plasmid

    #60526

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTriEx-NTOM20-mVenus-Zdk1

    Plasmid

    #81010

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CaV2.1/pcDNA3.1 ~ Human

    Plasmid

    #140575

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    CaV2.1 Human (CACNA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LSL-Cas9-Rosa26TV

    Plasmid

    #61408

    Purpose

    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    3xFLAG and P2A

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CAG

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBS-U6-crRNA-empty

    Plasmid

    #134921

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    crRNA for CRISPR-Cas3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HA-HIF1alpha-wt-pBabe-puro

    Plasmid

    #19365

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt human cDNA

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV5B-HA-Smad2

    Plasmid

    #11734

    Depositor

    Jeff Wrana

    Article

    Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIRES2_EGFP_KCNQ1

    Plasmid

    #173161

    Purpose

    For mammalian expression of KCNQ1 and creating variants

    Depositor

    Al George

    Article

    Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

    Insert

    KCNQ1 (KCNQ1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge