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167,150 results

Showing: 5101 - 5150 of 167150 results

  5. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

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  10. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-472945

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  11. TFORF2775

    Plasmid
    #144234
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDM5C (KDM5C Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144234-plasmid-map-sequence-id-404121

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  13. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  14. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Eco31I
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165506-plasmid-map-sequence-id-338512

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  15. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  19. AAV-CAG-jGCaMP8m-WPRE

    Plasmid
    #179255
    Purpose
    AAV-mediated expression of GCaMP8m under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179255-plasmid-map-sequence-id-354032

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  26. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid
    #99280
    Purpose
    Can be used to generate AAV virus that will express mScarlet in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mScarlet
    Use
    AAV and Cre/Lox
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99280-plasmid-map-sequence-id-487711

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  35. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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Showing: 5101 - 5150 of 167150 results