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We narrowed to 18,511 results for: tec

Showing: 5301 - 5350 of 18511 results

  1. HypoxCR

    Plasmid
    #59946
    Purpose
    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.
    Depositor
    Anne Le
    Article
    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p
    Inserts
    GFP-Pest
    mCherry-Geminin
    Use
    Lentiviral
    Tags
    FLAG and PEST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and min CMV
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    59946-plasmid-map-sequence-id-228094

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  19. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  22. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  27. PHP.AX

    Plasmid
    #195218
    Purpose
    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)
    Depositor
    Viviana Gradinaru
    Article
    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103
    Insert
    Synthetic construct isolate AAV-PHP.AX VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p41
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195218-plasmid-map-sequence-id-385556

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  30. pCB578

    Plasmid
    #141095
    Purpose
    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array
    Depositor
    Chase Beisel
    Article
    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d
    Inserts
    Cas9 and tracrRNA
    repeat-spacer-repeat array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141095-plasmid-map-sequence-id-292312

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  38. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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  40. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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  46. JD633

    Plasmid
    #160393
    Purpose
    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning
    Depositor
    Jorge Dubcovsky
    Article
    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103
    Inserts
    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    Use
    Binary vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160393-plasmid-map-sequence-id-456253

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Showing: 5301 - 5350 of 18511 results