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162,006 results

Showing: 5301 - 5350 of 162006 results

  2. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

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  6. pAAV-TetON-Bmpr1A-ca

    Plasmid
    #217536
    Purpose
    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    constitutively active BMP receptor 1a (BMPR1A Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Tet operon (TetO)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217536-plasmid-map-sequence-id-429501

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  13. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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  15. pMTMVi-N

    Plasmid
    #118756
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118756-plasmid-map-sequence-id-236109

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  20. pHnCBS1D

    Plasmid
    #52065
    Purpose
    expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible
    Depositor
    David Savage
    Article
    Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10
    Insert
    CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    52065-plasmid-map-sequence-id-80268

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  22. TIRAP-GFP

    Plasmid
    #52739
    Purpose
    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pPEE7

    Plasmid
    #128357
    Purpose
    Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128357-plasmid-map-sequence-id-254608

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  32. pBabe-CD44v4

    Plasmid
    #107969
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v4 (Cd44 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107969-plasmid-map-sequence-id-222222

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  33. pJYScr

    Plasmid
    #132719
    Purpose
    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance
    Depositor
    Jan Marienhagen
    Article
    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    132719-plasmid-map-sequence-id-259037

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  37. TFORF3058

    Plasmid
    #144534
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EBF1 (EBF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144534-plasmid-map-sequence-id-404210

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  38. TFORF2918

    Plasmid
    #142172
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIB (NFIB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142172-plasmid-map-sequence-id-397711

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  40. TFORF2528

    Plasmid
    #144127
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ERCC6 (ERCC6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    144127-plasmid-map-sequence-id-400934

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  46. pUltra-sY

    Plasmid
    #82417
    Purpose
    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Methanococcus jannaschii aaRS for sulfotyrosine
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…
    Promoter
    tacI
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    82417-plasmid-map-sequence-id-156040

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  47. pRYG

    Plasmid
    #113642
    Purpose
    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113642-plasmid-map-sequence-id-214560

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  48. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66

    Plasmid
    #111735
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q66 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111735-plasmid-map-sequence-id-226804

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Showing: 5301 - 5350 of 162006 results