Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

165,965 results

Showing: 5401 - 5450 of 165965 results

  1. pDestTol2CG2-U6:gRNA

    Plasmid
    #63156
    Purpose
    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63156-plasmid-map-sequence-id-177022

    Enlarge

  4. pWPXL

    Plasmid
    #12257
    Purpose
    2nd generation lentiviral transfer plasmid
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12257-plasmid-map-sequence-id-237089

    Enlarge

  10. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Purpose
    Cre-dependent AAV-mediated expression of tdTomato under the CAG promoter
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available Since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    28306-plasmid-map-sequence-id-162239

    Enlarge

  15. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

    Enlarge

  16. mCherry-ZO1-C-14

    Plasmid
    #55165
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55165-plasmid-map-sequence-id-194785

    Enlarge

  17. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

    Enlarge

  18. ADRB3-DuET

    Plasmid
    #213182
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB3 (ADRB3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213182-plasmid-map-sequence-id-430749

    Enlarge

  33. pUC57-LbNOX

    Plasmid
    #75285
    Purpose
    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    LbNOX
    Tags
    C-terminal FLAG tag with linker
    Mutation
    Human codon optimized
    Available Since
    May 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    75285-plasmid-map-sequence-id-258257

    Enlarge

  38. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid
    #174659
    Purpose
    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    Xon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174659-plasmid-map-sequence-id-351621

    Enlarge

  42. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50475-plasmid-map-sequence-id-171072

    Enlarge

Showing: 5401 - 5450 of 165965 results