159,238 results

1. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. phDLX2-N174

   Plasmid

   #60860

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   DLX2 (DLX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIB184 (EM)

   Plasmid

   #90194

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #213481

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. elavl3:hArr-TEV;he1.1:YFP

   Plasmid

   #218855

   Purpose

   D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Myo 19

   Plasmid

   #134987

   Purpose

   Expression of human Myo 19 in mammalian cells.

   Depositor

   Martin Bähler

   Article

   Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

   Insert

   Myo 19 (MYO19 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

   Plasmid

   #175037

   Purpose

   Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   Sp sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8.17-m

   Plasmid

   #136298

   Purpose

   expresses ABE8.17-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.17-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-DsRed_IRES_SNCA:EGFP

    Plasmid

    #92195

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-SNCA-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pKM495 (Flag-Das+4) Zeo

    Plasmid

    #109284

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-DAS+4
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218857

    Purpose

    Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Use

    Tags

    QF fusion protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Human, Schistosoma japonicum)
    

    Use

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCTIP2-N106

    Plasmid

    #66808

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    CTIP2 isoform b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1-hMcl-1

    Plasmid

    #25375

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. MYC-hTRAK2

    Plasmid

    #225142

    Purpose

    Expresses MYC-tagged human TRAK2

    Depositor

    Thomas Schwarz

    Article

    Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

    Insert

    TRAK2 (TRAK2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-mCherry

    Plasmid

    #50462

    Purpose

    Double floxed mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mNeptune2-N1

    Plasmid

    #54837

    Purpose

    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mNeptune2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pRRL-TetO-Pax7-IRES-EGFP

    Plasmid

    #109372

    Purpose

    Expresses Dox-inducible Pax7 together with a GFP reporter

    Depositor

    Nenad Bursac

    Article

    Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

    Insert

    Pax7
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK5-myc-Miro2

    Plasmid

    #47891

    Purpose

    Expresses myc tagged Miro2

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro2 (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pORANGE GFP-Grin1 KI

    Plasmid

    #131485

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTEC27

    Plasmid

    #30182

    Depositor

    Lalita Ramakrishnan

    Article

    Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-PKM2-WT

    Plasmid

    #122690

    Purpose

    Express His-tagged PKM2 (wild type) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR.hU6hH1

    Plasmid

    #32686

    Purpose

    dual shRNA expression cassette entry vector

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6hH1

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR4_G3BP1

    Plasmid

    #127104

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Pb-TRE3G-SpCas9-IRES-blast

    Plasmid

    #195506

    Purpose

    Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Oct4-IRES-eGFP-PGK-Neo

    Plasmid

    #48681

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD_TypeIC_Cascade_cas3_invivo

    Plasmid

    #196400

    Purpose

    expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Inserts

    Cas5c
    Cas7c
    Cas8c
    Cas3c
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-MS2-HA-HsNBAS_AA

    Plasmid

    #148236

    Purpose

    Mammalian Expression of HsNBAS

    Depositor

    Elisa Izaurralde

    Insert

    HsNBAS (NBAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge