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pC013 - Twinstrep-SUMO-huLwCas13a

Plasmid

#90097

Purpose

Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a

Use

CRISPR

Tags

6xHis-Twin Strep-SUMO

Expression

Bacterial

Available since

June 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

34 Articles

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pLV-EF1a-IRES-Puro

Plasmid

#85132

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

94 Articles

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

51 Articles

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pBS-CMV-gagpol

Plasmid

#35614

Depositor

Patrick Salmon

Insert

MLV gag and pol genes

Expression

Mammalian

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pLenti CMV Blast DEST (706-1)

Plasmid

#17451

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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pUltra

Plasmid

#24129

Purpose

3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

Depositor

Malcolm Moore

Publication

Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCAG-Flpe

Plasmid

#13787

Purpose

Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pcDNA3-EGFP-RhoA-Q63L

Plasmid

#12968

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA constitutively active (RHOA Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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IFN-Beta_pGL3

Plasmid

#102597

Purpose

IFN-beta promoter driving luciferase. Use in IFN reporter assay.

Depositor

Nicolas Manel

Publication

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

Human IFN-Beta promoter (IFNB1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Promoter

dSV40 Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Publication

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pEVOL-pBpF

Plasmid

#31190

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

Depositor

Peter Schultz

Publication

Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

M.j. p-benzoylphenylalanine RS (2 copies +tRNA)

Expression

Bacterial

Mutation

Y32G, E107P, D158T, I159S

Available since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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pLVUT-tTR-KRAB

Plasmid

#11651

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Publication

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAdTrack-CMV

Plasmid

#16405

Purpose

Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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35S:RUBY

Plasmid

#160908

Purpose

RUBY under the control of CaMV 35S promoter/marker for transformation

Depositor

Yunde Zhao

Publication

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and CaMV 35S promoter

Expression

Plant

Promoter

CaMV 35S

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pCAG-EGxxFP

Plasmid

#50716

Purpose

5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Publication

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

split EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

63 Articles

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DsRed-rab7 WT

Plasmid

#12661

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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GFP-p53

Plasmid

#12091

Depositor

Tyler Jacks

Publication

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

p53 (TP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Available since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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GW1-PercevalHR

Plasmid

#49082

Purpose

Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Expression

Mammalian

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Publication

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

192 Articles

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pLIX_403

Plasmid

#41395

Purpose

Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

Depositor

David Root

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

V5

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

74 Articles

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Ub-G76V-GFP

Plasmid

#11941

Depositor

Nico Dantuma

Publication

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-G76V-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pCS2-nCas9n

Plasmid

#47929

Purpose

expression of an optimized Cas9 for genome-editing in zebrafish

Depositor

Wenbiao Chen

Publication

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

nls-zcas9-nls

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

104 Articles

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FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

81 Articles

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pAdEasy-1

Plasmid

#16400

Purpose

Adenoviral vector for use in AdEasy System.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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piRFP

Plasmid

#31857

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Publication

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Available since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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