8,962 results

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AAV-hSyn-Soma-jGCaMP8s

Plasmid

#169256

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PB-sgRNA(MS2)

Plasmid

#102560

Purpose

Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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myc-Raptor

Plasmid

#1859

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Raptor (RPTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5M-ADAM10

Plasmid

#31717

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

ADAM10 (ADAM10 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-mCherry

Plasmid

#137134

Purpose

Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hOCT3/4

Plasmid

#17217

Purpose

Retroviral expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

OCT3/4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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V406 4HA-CKIe(K38R)

Plasmid

#13725

Depositor

David Virshup

Article

Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

Insert

casein kinase 1 epsilon (CSNK1E Human)

Tags

4 HA

Expression

Mammalian

Mutation

kinase-inactive due to K38R mutation

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pAL120-TK

Plasmid

#22053

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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GSK3beta (1015)

Plasmid

#49491

Purpose

expresses human Gsk3beta

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta (GSK3B Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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mRFP-wGBD

Plasmid

#26733

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

mRFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Available Since

April 21, 2011

Availability

Academic Institutions and Nonprofits only
Tet-on circRNA Expression Vector

Plasmid

#92351

Purpose

The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

Depositor

Ling-Ling Chen

Article

Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

Insert

mCherry and split EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

pTet

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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NLSx3-CRY2PHR-p65

Plasmid

#64124

Purpose

Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

CRY2PHR-p65

Tags

His, NLSx3, and V5

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLOX-CWBmi1

Plasmid

#12240

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

Bmi1 (Bmi1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter upstream of mBmi-1. LoxP in 3' …

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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fos-tTA

Plasmid

#34856

Depositor

Mark Mayford

Article

Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

Insert

Fos promoter/first intron -764/+918 (Fos Mouse)

Use

Mouse Targeting

Tags

Fos 3' UTR (~1500bp) and tTA (tetracycline-c…

Expression

Mammalian

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Tags

sfCherry11x4

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5

Plasmid

#19445

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

V5

Expression

Mammalian

Mutation

V5 tag inserted to create new vector

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB533

Plasmid

#74872

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP Kindlin2

Plasmid

#105305

Purpose

To study effect of Kindlin 2 on Cell/ECM adhesion

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe puro c-met WT

Plasmid

#17493

Depositor

Joan Brugge

Article

Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

Insert

c-Met (Met Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-cdh1

Plasmid

#11596

Depositor

Marc Kirschner

Article

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdh1 (FZR1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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HA-Foxo1ADA (pCMV5)

Plasmid

#12143

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 ADA (Foxo1 Mouse)

Tags

HA and myc

Expression

Mammalian

Mutation

Phosphorylation-deficient, also referred to as co…

Promoter

CMV

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-GATA2

Plasmid

#1418

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 TGF beta type I receptor Flag

Plasmid

#14831

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

flag

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ASH1L

Plasmid

#65369

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ASH1L (ASH1L Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR CD19 tandem CAR

Plasmid

#113019

Purpose

for lentivirus production of CD19 tandem CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Synthetic, Human, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-TRIM28

Plasmid

#65397

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM28 (TRIM28 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pAG423GAL-ccdB

Plasmid

#14149

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

Plasmid

#63931

Purpose

Empty AAV-RAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-PE2-C

Plasmid

#164908

Purpose

Expresses the C-terminal split-intein fragment of PE2

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

PE2-C

Use

AAV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC7-20XUAS-IVS-mCD8::GFP

Plasmid

#26220

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC hRap1-FL

Plasmid

#12542

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 (TERF2IP Human)

Use

Retroviral

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
LeGO-C2

Plasmid

#27339

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/HA-FLAG-RBP2

Plasmid

#14800

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-BRD4ΔN-mCherry-sspB

Plasmid

#121968

Purpose

Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAGFP-N1

Plasmid

#11909

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

pPAGFP-N1

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GATA2

Plasmid

#1287

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
dClover2-N1

Plasmid

#54538

Purpose

Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pL3-TRE-LucGFP-2L

Plasmid

#11685

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Insert

Luciferase-EGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,962 results