8,631 results

1. ACE2(-1119)-luc

   Plasmid

   #31110

   Purpose

   Luciferase reporter construct for human ACE2 promoter

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

   Insert

   ACE2 promoter (ACE2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   promoter region -1119 to +103

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAAV-hSyn-GRAB_NE1m

   Plasmid

   #123308

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains a glycine-to-threonine mutation at posit…

   Promoter

   hSyn

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p415-GalL-Cas9-CYC1t

   Plasmid

   #43804

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GalL

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C1-TBP

   Plasmid

   #26674

   Depositor

   Sui Huang

   Article

   Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

   Insert

   TBP (TBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYFP-RPB1aAmr

   Plasmid

   #75284

   Purpose

   Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

   Depositor

   Robert Singer, Xavier Darzacq

   Article

   Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   N792D alpha-amanitin–resistant

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-FLAG-Lin28A

   Plasmid

   #51371

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   Lin28A (LIN28A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGTQL1221YC

   Plasmid

   #61705

   Purpose

   Gateway compatible BiFC vector (pEarleyGate202-YC)

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Bifc assay

   Tags

   YFP-C and FLAG

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tol2-elavl3-H2B-GCaMP6s

   Plasmid

   #59530

   Purpose

   Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

   Depositor

   Misha Ahrens

   Article

   Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

   Inserts

   elavl3 promoter (elavl3 Zebrafish)
   H2B
   GCaMP6s
   

   Use

   Transposable element

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMCP-RecT

   Plasmid

   #183827

   Purpose

   pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   MCP-RecT_NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-PE2-BSD

    Plasmid

    #161514

    Purpose

    Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

    Insert

    PE2-P2A-BSD
    

    Use

    Lentiviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR1A-Huwe1

    Plasmid

    #37431

    Depositor

    Jean Cook

    Insert

    Huwe1 (HUWE1 Human)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-mMaroon1

    Plasmid

    #83840

    Purpose

    Expresses mMaroon1

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71761

    Purpose

    mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG426GAL-ccdB

    Plasmid

    #14155

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQCXIH-Myc-YAP-5SA

    Plasmid

    #33093

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Promoter

    CMV/MSV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

    Plasmid

    #92143

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hDlx-GqDREADD-dTomato-Fishell-4

    Plasmid

    #83897

    Purpose

    Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    hM3Dq
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hDlx

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid

    #106174

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1 and AAV9

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSET YBX-1

    Plasmid

    #13035

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

    Insert

    YBX1 (YBX1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCS2 Flag-JunB

    Plasmid

    #29687

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    jun B oncogene (JUNB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDH-PGK

    Plasmid

    #72268

    Purpose

    Express gene of interest under PGK (phosphoglycerate kinase I) promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pcDNA3-TLR10-YFP

    Plasmid

    #13643

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 10 (TLR10 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCold-I-Dsup

    Plasmid

    #90021

    Purpose

    To express His-tagged Dsup in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV Sport2 mPer2

    Plasmid

    #16204

    Depositor

    Cheng Lee

    Article

    Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

    Insert

    Per2 (Per2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

32. pDONR223_CTCF_WT

    Plasmid

    #81789

    Purpose

    Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CTCF (CTCF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTP943

    Plasmid

    #104157

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fab specific nanobody TP886
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-MEF2C-HA

    Plasmid

    #32515

    Depositor

    Andrew Lassar

    Article

    Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

    Insert

    Mef2c (Mef2c Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BDNF-SEP

    Plasmid

    #83955

    Purpose

    BDNF with pH sensitive fluorophore for visualization of release

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Brain derived neurotrophic factor (Bdnf Mouse)
    

    Use

    Tags

    SEP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Casp7-Flag

    Plasmid

    #11815

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 7 (CASP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAVS1-P-CAG-GFPluc2

    Plasmid

    #80493

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    GFPluc2
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PSD-95 FLAG

    Plasmid

    #15463

    Depositor

    Wei-dong Yao

    Article

    Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FLAG-SNAP29

    Plasmid

    #45915

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    synaptosomal-associated protein, 29kDa (SNAP29 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pU6-sp-sgRNA-RNF2_+41nick

    Plasmid

    #135958

    Purpose

    S. pyogenes sgRNA for +41 nick in RNF2

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_+41 nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. HO-Poly-KanMX4-HO

    Plasmid

    #51662

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBa-MARK2-eGFP

    Plasmid

    #66706

    Purpose

    mammalian expression vector for MARK2 with a C-terminal eGFP

    Depositor

    Marvin Bentley, Gary Banker

    Insert

    MARK2 (MARK2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGEMHE-mEGFP-mTrim21

    Plasmid

    #105519

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. HIV-1 Gag-mCherry

    Plasmid

    #85390

    Purpose

    Visualization marker for viral core to measure cellular uptake

    Depositor

    Gregory Melikyan

    Article

    Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

    Insert

    HIV-1 gag
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. p3113 GST-p53

    Plasmid

    #10852

    Depositor

    Peter Howley

    Article

    Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLenti6-DEST PINK1-V5 WT

    Plasmid

    #13320

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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50. pJ3H-Mst1

    Plasmid

    #12203

    Depositor

    Jonathan Chernoff

    Article

    Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

    Insert

    Mst1 (STK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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