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169,365 results

Showing: 5751 - 5800 of 169365 results

  4. pRRLsinPGK_IRFP720_WPRE

    Plasmid
    #91785
    Purpose
    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    iRFP720
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91785-plasmid-map-sequence-id-180617

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  11. CMV-Y-GECO1

    Plasmid
    #55766
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Tags
    6x His tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55766-plasmid-map-sequence-id-89442

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  13. pRK5F-ALK5-HA(ecto)

    Plasmid
    #31720
    Depositor
    Rik Derynck
    Insert
    ALK5 (TGFBR1 Human)
    Tags
    FLAG and HA
    Expression
    Mammalian
    Available Since
    Feb. 14, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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  22. HTR2B-DuET

    Plasmid
    #213322
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2B (HTR2B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213322-plasmid-map-sequence-id-430858

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  25. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  31. pMal-T-Avi-His/BirA

    Plasmid
    #102962
    Purpose
    Vector for in vivo biotinylation of a fusion protein in bacteria.
    Depositor
    Tonia Rex
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    102962-plasmid-map-sequence-id-199098

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  32. pAAV.TBG.PI.Null.bGH

    Plasmid
    #105536
    Purpose
    empty AAV vector with TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    None
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TBG
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105536-plasmid-map-sequence-id-202373

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  46. pLenti-mCherry-Cre-blast

    Plasmid
    #179390
    Purpose
    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    mCherry (P2A) Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179390-plasmid-map-sequence-id-451660

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Showing: 5751 - 5800 of 169365 results