163,760 results

1. pCDH-EF1-Luc2-P2A-copGFP

   Plasmid

   #72485

   Purpose

   Express Luc2 and copGFP

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pSB219

   Plasmid

   #164125

   Purpose

   p15A backbone with PhlF binding sites

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-TLR9-YFP

   Plasmid

   #13642

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 9 (TLR9 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 40 pCOLI_K_MBP

   Plasmid

   #160183

   Purpose

   N-term MBP 3C cleavable Kan resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti6.2-3xFLAG-V5-ccdB

   Plasmid

   #87072

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

   Depositor

   Susan Lindquist, Mikko Taipale

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T1-Rpb4/7

    Plasmid

    #138484

    Purpose

    Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Inserts

    Rpb4 (POLR2D Human)
    Rpb7 (POLR2G Human)
    

    Use

    Tags

    6xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR L3-4XmCherry-HA-L2

    Plasmid

    #32315

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    4XmCherry-HA
    

    Use

    Gateway multisite entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mc2 mCherry

    Plasmid

    #206219

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-SENP1

    Plasmid

    #17357

    Depositor

    Edward Yeh

    Article

    Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

    Insert

    SENP1 (SENP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF1918

    Plasmid

    #142886

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX3 (RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBA559

    Plasmid

    #186235

    Purpose

    pTet-LbuCas13a crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mc2 neo

    Plasmid

    #206214

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LeGO-V2

    Plasmid

    #27340

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, Venus fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 5 pCOLI_A_STREP

    Plasmid

    #160147

    Purpose

    N-term 2xStrepII 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcCas12nHS_RV6_PT1_empty

    Plasmid

    #203810

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6_PT1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDuet1_CoiC_CoiSA

    Plasmid

    #208762

    Purpose

    Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiC
    CoiSA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    single nucleotide insertion (C70) at RBS of CoiC

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCHW101020

    Plasmid

    #222454

    Purpose

    Level 1, Position 2 containing 35S::CUP2-p65::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    35S::CUP2-p65::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF0207

    Plasmid

    #141555

    Purpose

    Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSH87

    Plasmid

    #127705

    Purpose

    Pflp-13::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-13

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-GRAB-gDA3h

    Plasmid

    #208699

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mRFP-Rab5

    Plasmid

    #14437

    Purpose

    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 5 (RAB5A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSH88

    Plasmid

    #127703

    Purpose

    Pacr-2::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    acr-2

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[3]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST-DHFR F[1,2]-C (TRP1)

    Plasmid

    #177795

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TAL2289

    Plasmid

    #36724

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCG-TALEN_Right (Fancg Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

30. 37 pCOLI_K_STREP

    Plasmid

    #160263

    Purpose

    N-term 2xStrepII 3C cleavable Kan resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACyR1

    Plasmid

    #172565

    Purpose

    Inducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    CymRAM-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCymRC

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA-KIF5A

    Plasmid

    #166958

    Purpose

    Expresses HA-tagged KIF5A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQLink-GST-4xCTD

    Plasmid

    #138471

    Purpose

    Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    4x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSH79

    Plasmid

    #127704

    Purpose

    Pttr-39::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    ttr-39

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSH86

    Plasmid

    #127706

    Purpose

    Pmyo-3::GFP1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CROP-seq-opti-dsRed

    Plasmid

    #201999

    Purpose

    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

    Depositor

    Daniel Geschwind

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSLQ14902

    Plasmid

    #239277

    Purpose

    Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PspCas13b-hGAPDH-gRNA2 (GAPDH Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSLQ10622

    Plasmid

    #239274

    Purpose

    Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLQ10550

    Plasmid

    #239269

    Purpose

    Expresses PYL1-MAVS* for recruiting RNA to the outer mitochondrial membrane via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-MAVS(aa510-540) (MAVS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSLQ10552

    Plasmid

    #239271

    Purpose

    Expresses PYL1-G3BP1 for recruiting RNA to the stress granule via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEMTB-mScarlet3-H (aka mYongHong)

    Plasmid

    #239407

    Purpose

    Expresses EMTB-mScarlet3-H in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    EMTB (MAP7 Human)
    

    Use

    Tags

    mScarlet3-H (aka mYongHong)

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTom20-mScarlet3-H (aka mYongHong)

    Plasmid

    #239412

    Purpose

    Expresses Tom20-mScarlet3-H in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mScarlet3-H (aka mYongHong)

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pNEU-KlacRS

    Plasmid

    #232155

    Purpose

    Site-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    KlacRS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA-mCherry-TAG-EGFP

    Plasmid

    #232156

    Purpose

    A fluorescence reporter to show incorporation of target unnatural amino acids.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    mCherry-linker(TAG)-EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEvol-MmKlacRS1

    Plasmid

    #232157

    Purpose

    Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    KlacRS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENTR_sfGFP-TurboID

    Plasmid

    #240226

    Purpose

    Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    sfGFP-TurboID
    

    Use

    Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pStayGold-Tubulin

    Plasmid

    #229571

    Purpose

    Expresses StayGold-tubulin fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    StayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pENTR2b-EGFP

    Plasmid

    #215537

    Purpose

    Gateway entry vector with the EGFP fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. FLuc-N-HiBiT

    Plasmid

    #207117

    Purpose

    Bacterially expressed HiBiT-FLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    luciferin 4-monooxygenase
    

    Use

    Tags

    HiBiT Tag and His-tag with thrombin site

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBIG1e-CDK12deltaRS/Cyclin K

    Plasmid

    #231744

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(delta104-380)

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge