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159,237 results

Showing: 5751 - 5800 of 159237 results

  5. pLJM1-EGFP-GRAM-W

    Plasmid
    #211704
    Purpose
    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211704-plasmid-map-sequence-id-419216

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  7. TFORF1884

    Plasmid
    #143984
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZIC2 (ZIC2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143984-plasmid-map-sequence-id-400555

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  9. pLV-TetO-hNGN2-Puro

    Plasmid
    #79049
    Purpose
    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Article
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TetO
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79049-plasmid-map-sequence-id-429069

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  10. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Promoter
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  11. pSR78

    Plasmid
    #90210
    Purpose
    Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA
    Depositor
    Jeffrey Tabor
    Article
    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do
    Inserts
    CcaS
    Cph8
    Ho1
    PcyA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90210-plasmid-map-sequence-id-178317

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  19. pcDNA3-Flag-Tet1 CD Mut

    Plasmid
    #72218
    Purpose
    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 1 (Tet1 Mouse)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET1 catalytic domain (cata…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. pSR83

    Plasmid
    #90211
    Purpose
    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter
    Depositor
    Jeffrey Tabor
    Article
    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do
    Inserts
    OmpR (ompR E. coli)
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90211-plasmid-map-sequence-id-178319

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  29. AbVec2.0-IGHG4

    Plasmid
    #99578
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99578-plasmid-map-sequence-id-192595

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  35. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114404-plasmid-map-sequence-id-220815

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  36. mCherry-CD36-C-10

    Plasmid
    #55011
    Purpose
    Localization: Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CD36 (CD36 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55011-plasmid-map-sequence-id-313445

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  39. pSLC-217

    Plasmid
    #73163
    Purpose
    Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-relE (relE E. coli (bacteria))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73163-plasmid-map-sequence-id-151344

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  42. ARHGEF2A-c055

    Plasmid
    #205103
    Purpose
    ARHGEF2 protein expression
    Depositor
    Paul Brennan
    Insert
    ARHGEF2 (ARHGEF2 Human)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205103-plasmid-map-sequence-id-412139

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  43. ARHGEF2A-c052

    Plasmid
    #205102
    Purpose
    ARHGEF2 protein expression
    Depositor
    Paul Brennan
    Insert
    ARHGEF2 (ARHGEF2 Human)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205102-plasmid-map-sequence-id-412138

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  44. RHOAA-c035

    Plasmid
    #205118
    Purpose
    RHOA protein expression
    Depositor
    Paul Brennan
    Insert
    RHOA (RHOA Human)
    Use
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205118-plasmid-map-sequence-id-411209

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  45. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #115344
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    115344-plasmid-map-sequence-id-222905

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  49. SPYtag-GFPnanobody

    Plasmid
    #214837
    Purpose
    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    SPYtag-GFPnanobody
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214837-plasmid-map-sequence-id-425310

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Showing: 5751 - 5800 of 159237 results