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177,170 results

Showing: 5751 - 5800 of 177170 results

  2. 1478 pcDNA3 flag HA 14-3-3 beta

    Plasmid
    #8999
    Depositor
    William Sellers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14-3-3 beta (YWHAB Human)
    Tags
    HA and flag
    Expression
    Mammalian
    Available since
    April 20, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  4. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SHP2 (PTPN11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles

  8. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid
    #171379
    Purpose
    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized Cas13X.1
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171379-plasmid-map-sequence-id-337344

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  10. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Publication
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Promoter
    T7, tetPA
    Available since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
    161875-plasmid-map-sequence-id-316881

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  13. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Publication
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    21 Articles
    155297-plasmid-map-sequence-id-307858

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  15. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Publication
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    133451-plasmid-map-sequence-id-267048

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  22. pB-CAGGS-dCas9-KRAB

    Plasmid
    #110822
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    110822-plasmid-map-sequence-id-265907

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  25. pEGFP-h53BP1 (siRNA resistant)

    Plasmid
    #110301
    Purpose
    Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)
    Depositor
    Chris Kok-Lung Chan
    Publication
    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p53-binding protein 1 (TP53BP1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    GAACGAGGA to GAGCGGGGC
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    110301-plasmid-map-sequence-id-387855

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  27. pWS158

    Plasmid
    #90517
    Purpose
    Cas9 gap repair vector - URA3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    90517-plasmid-map-sequence-id-287206

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  29. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Publication
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    92073-plasmid-map-sequence-id-275942

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  32. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    84239-plasmid-map-sequence-id-160811

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  33. pLV_Zika_NS5_Flag

    Plasmid
    #79639
    Purpose
    Third generation Self-inactivating lentivector expressing NS5 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS5
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    79639-plasmid-map-sequence-id-148807

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  37. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

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  38. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    25 Articles
    72263-plasmid-map-sequence-id-474982

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  39. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    72248-plasmid-map-sequence-id-131868

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  42. pMito-rxRFP1.1

    Plasmid
    #67841
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized
    Depositor
    Huiwang Ai
    Publication
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rxRFP1.1 and mitochondrial localization sequence
    Tags
    His Tag and mitochondrial localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    67841-plasmid-map-sequence-id-244286

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  46. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65772-plasmid-map-sequence-id-112654

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  48. pVITRO1-Trastuzumab-IgG2/κ

    Plasmid
    #61884
    Purpose
    Expresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61884-plasmid-map-sequence-id-207869

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  49. GFP-Rab4B

    Plasmid
    #61801
    Purpose
    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression
    Depositor
    Gia Voeltz
    Publication
    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab4B (RAB4B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61801-plasmid-map-sequence-id-454375

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  50. GFP-CHC17KDP

    Plasmid
    #59799
    Purpose
    Expresses knockdown-proof human CHC17 tagged with GFP.
    Depositor
    Stephen Royle
    Publication
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHC17 (CLTC Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 61-65 to confer s…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    59799-plasmid-map-sequence-id-507236

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Showing: 5751 - 5800 of 177170 results