165,343 results

1. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

   Plasmid

   #229677

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   SNAP, 3xV5

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-rtTA3G

   Plasmid

   #139556

   Purpose

   rtTA ORF with stop codon between attL1-attL2 on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   rtTA3G
   

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pvimentin-emiRFP703

   Plasmid

   #136566

   Purpose

   Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   vimentin-emiRFP703
   

   Tags

   emiRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221_SLC15A4

   Plasmid

   #131910

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC15A4 (SLC15A4 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET Flag TEV LIC cloning vector (1L)

   Plasmid

   #29662

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   Flag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn7-132

   Plasmid

   #118550

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn7-135

   Plasmid

   #118553

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2895

   Plasmid

   #144355

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MTA1 (MTA1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-Flag-APH4-Fibcon-icam

    Plasmid

    #233077

    Purpose

    Expresses synCAM-APH4-Fibcon-icam

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    APH4-FIBCON-ICAM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR3.1-OPNa

    Plasmid

    #107980

    Purpose

    expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    full-length osteopontin (SPP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG5-His-SUMO1

    Plasmid

    #17271

    Depositor

    Stephen Goff

    Article

    Yueh et al J Virol. 2006 Jan . 80(1):342-52.

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-SEP-Glur2 (MCA10)

    Plasmid

    #64941

    Purpose

    mammalian expression of rat GluR2

    Depositor

    Jeremy Henley

    Article

    Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

    Insert

    GluR2 (Gria2 Rat)
    

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK1as_T2A_puromycin

    Plasmid

    #118595

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p413 TEF NAPstar3

    Plasmid

    #232740

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar3
    

    Expression

    Yeast

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS59

    Plasmid

    #215680

    Purpose

    Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. HIV-1 integrase F185K/C280S in pET-15b

    Plasmid

    #61669

    Purpose

    HIV-1 integrase F185K/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185K/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Promoter

    T7 promotor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pWay21-MKP-1 FL

    Plasmid

    #13469

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1 FL (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pPBpuro-miRFP670-HRasCT

    Plasmid

    #176030

    Purpose

    Encoding membrane-targeted miRFP670

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Membrane-targeted miRFP670
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pegRNA-GBA-10

    Plasmid

    #199271

    Purpose

    pegRNA used to induce GBA (c. 1226 A > G) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    GBA 1226AtoG pegRNA
    

    Expression

    Mammalian

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBAD-HisD-CreiLOV

    Plasmid

    #192817

    Purpose

    Expresses CreiLOV in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMal-Strep

    Plasmid

    #209345

    Purpose

    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII

    Depositor

    Lucia Strader

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCG_IFITM1-IRES_eGFP

    Plasmid

    #179958

    Purpose

    Expression vector for human IFITM1. Co-expresses GFP controlled by an IRES element.

    Depositor

    Frank Kirchhoff, Konstantin Sparrer

    Article

    Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

    Insert

    IFITM1 (IFITM1 Human)
    

    Expression

    Mammalian

    Mutation

    P13A

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. p5E TRE-3GV (JDW 1163)

    Plasmid

    #224476

    Purpose

    Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TRE3GV promoter
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMXs-IRES-blast GFP-SPNS1

    Plasmid

    #198571

    Purpose

    SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDONR223_CSNK1A1_WT

    Plasmid

    #82106

    Purpose

    Gateway Donor vector containing CSNK1A1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Gateway entry vector

    Mutation

    Q231K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEditA

    Plasmid

    #207531

    Purpose

    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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