170,823 results
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Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
ExpressionBacterialPromoterN/AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
TagsroGFP2 fusion with Tsa2dCrExpressionYeastPromoterTEFAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-EGFP-PRC1
Plasmid#167540PurposeFor expression of His6-TEV-EGFP-PRC1 in bacteria.DepositorAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-KRAS-G12D
Plasmid#179656PurposeKRAS active mutant (G12D mutation)DepositorInsertKRAS G12D
ExpressionMammalianMutationG12DAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-KRAB-Puro
Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-1:PXN-EGFP
Plasmid#87420PurposeHomology arms and linker-EGFP sequence for C-terminus tagging of human PXNDepositorInsertPXN Homology Arms with linker-EGFP (PXN Human)
UseCRISPR; Donor templateTagslinker-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMK465 (mAB-Hygro)
Plasmid#214391PurposemAID-BromoTag for C-terminal taggingDepositorInsertmAID-BrpmpTag-Hygro
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2-mDux
Plasmid#138320PurposeDox-inducible codon-optimized mouse DuxDepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FURIN_WT
Plasmid#82122PurposeGateway Donor vector containing FURIN , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pP2A-mCherry-N1
Plasmid#84329PurposeEncodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherryDepositorInsertP2A-mCherry
ExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Myt1l
Plasmid#27152DepositorAvailable SinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS MCK promoter
Plasmid#12528DepositorInsertMCK promoter (Ckm Mouse)
ExpressionMammalianAvailable SinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRint
Plasmid#24218DepositorInsertRFP with modified adeno intron
ExpressionMammalianMutationdsRED interrupted by a modified adenovirus intronAvailable SinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
Tags6His, TEV siteExpressionBacterialPromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smad3
Plasmid#11742DepositorAvailable SinceAug. 7, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLBPVXBa-M
Plasmid#229079PurposeNew version of a PVX vector to express heterologous sequences in plantsDepositorInsertPotato virus X
ExpressionPlantMutationMutation coat protein start codon (ATG to AGG); d…Available SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK464 (mAB-Neo)
Plasmid#214390PurposemAID-BromoTag for C-terminal taggingDepositorInsertmAID-BromoTag-Neo
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nluc CD63
Plasmid#242528PurposeThis plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.DepositorAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
FRE5Cf
Plasmid#62378PurposeDerived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.DepositorInsertdsRed and GFP
ExpressionMammalianAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-oScarlet-WPRE-BC12-pA
Plasmid#220776PurposeExpression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertoScarlert-WPRE-BC12
UseAAVPromoterCAGAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-hCD19
Plasmid#127889PurposeExpresses hCD19DepositorAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DCAF7:1-342
Plasmid#211118PurposeMammalian expression of full-length human DCAF7. N-terminal HiBiT tag.DepositorInsertDCAF7:M1-V342 (DCAF7 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N2-COL1A1
Plasmid#66602PurposeExpresses procollagen 1 alpha 1 as a GFP fusionDepositorInsertCOL1A1 (COL1A1 Human)
ExpressionMammalianAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato
Plasmid#28306PurposeCre-dependent AAV-mediated expression of tdTomato under the CAG promoterDepositorHas ServiceAAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserttdTomato
UseAAV and Cre/Lox; Adeno-associated virusExpressionMammalianMutationN/APromoterCAGAvailable SinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pB-nCas9-PBE
Plasmid#98160PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
HIV7-Bcl2-CD28-CD3z-T2A-EGFR
Plasmid#163465PurposeLentiviral vector encoding a Bim-specific chimeric antigen receptor with a synthetic Bcl2 binding elementDepositorInsertBim-specific CAR
UseLentiviralTagsHAAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2-WPRE
Plasmid#218874PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2
UseAAVExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Paxillin-pEGFP
Plasmid#15233DepositorAvailable SinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHL-FcHis
Plasmid#99846Purposemammalian expression with secretive signal and FC tagDepositorAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry
Plasmid#92202PurposeFluorescent protein reporter constructDepositorInsertmCherry
UseAAVExpressionMammalianPromoterTREAvailable SinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGY037 BSMV-gamma-AmCyan
Plasmid#234818PurposeTo express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)DepositorInsertAmCyan
ExpressionPlantAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA
Plasmid#178534PurposeModifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)DepositorTypeEmpty backboneTagsHAExpressionMammalianPromoterEF1aAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-BioID2-HA
Plasmid#176744PurposeExpresses Ezrin fused with BioID2 at the C-terminus in astrocytesDepositorInsertEzr-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK466 (mAB-BSD)
Plasmid#214392PurposemAID-BromoTag for C-terminal taggingDepositorInsertmAID-BromoTag-BSD
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
hEGFR-mEGFP
Plasmid#182878PurposeMammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFPDepositorInserthEGFR-mEGFP (EGFR Human)
ExpressionMammalianAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T
Plasmid#236727PurposeBrain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.DepositorInsertscis-regulatory elements 89763
iCre(R297T)
4x6T miRNA targeting cassette
UseAAVMutationR297TAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloCKAR2.2-T2A-EGFP
Plasmid#216493PurposeExpression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloCKAR2.2
TagsT2A-EGFPExpressionMammalianPromoterCMVAvailable SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE
Plasmid#198515PurposeExpresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianPromoterEF-1aAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_128
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
TagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
ADORA3-Tango
Plasmid#66212PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH6-MBP-TEV-BirA
Plasmid#179694PurposeExpresses His6 tagged MBP-BirA fusion proteinDepositorInsertBirA
TagsHis6-MBPExpressionBacterialAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp41
Plasmid#228207PurposeExpression in BL21(DE3) of T4 helicaseDepositorInsert41 (41 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp59
Plasmid#228206PurposeExpression in BL21(DE3) of WT T4 helicase loaderDepositorInsert59 (59 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only