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163,570 results

Showing: 6101 - 6150 of 163570 results
  1. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pCMV-15F11-HA-mCh

    Plasmid
    #129591
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Insert
    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-HA-SOX4

    Plasmid
    #110367
    Purpose
    Mammalian expression of HA-tagged SOX4
    Depositor
    Insert
    SOX4 (SOX4 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid
    #181975
    Purpose
    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Insert
    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NGFR truncation: aa 11-276
    Promoter
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid
    #208717
    Purpose
    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons
    Depositor
    Has Service
    AAV1, AAV2, and AAV8
    Insert
    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. GST-PR-LBD

    Plasmid
    #89117
    Purpose
    bacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1
    Depositor
    Insert
    human progesterone receptor ligand binding domain (PGR Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains Ligand Binding Domain (637-933)
    Promoter
    tac
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 6101 - 6150 of 163570 results