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179,521 results

Showing: 5041 - 5080 of 179521 results

  1. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-525961

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  5. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139988-plasmid-map-sequence-id-275338

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  7. pLifeAct-HyPer7

    Plasmid
    #136464
    Purpose
    Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136464-plasmid-map-sequence-id-271538

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  10. pDisplay-GACh2.0

    Plasmid
    #106073
    Purpose
    express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based ACh sensor (GACh) 2.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106073-plasmid-map-sequence-id-260902

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  11. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104567-plasmid-map-sequence-id-201429

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  12. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid
    #100843
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100843-plasmid-map-sequence-id-447138

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  13. Ezh2[FL]-dCas9

    Plasmid
    #100086
    Purpose
    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14056 (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[FL]-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100086-plasmid-map-sequence-id-197550

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  14. pCMV-pro-Il1b-C-Flag

    Plasmid
    #75131
    Purpose
    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    interleukin 1 beta (Il1b Mouse)
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    (please see depositor comments below)
    Promoter
    CMV
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75131-plasmid-map-sequence-id-192693

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  21. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    72268-plasmid-map-sequence-id-134573

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  23. GFP_CD47_LU

    Plasmid
    #65474
    Purpose
    GFP tagged CD47 with its own long 3'UTR
    Depositor
    Christine Mayr
    Publication
    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    Expression
    Mammalian
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65474-plasmid-map-sequence-id-117297

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  25. GFP-PBRM1

    Plasmid
    #65387
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65387-plasmid-map-sequence-id-322048

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  26. GFP-BRD3

    Plasmid
    #65377
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRD3 (BRD3 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65377-plasmid-map-sequence-id-287374

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  29. mCherry-Beta-Catenin-20

    Plasmid
    #55001
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55001-plasmid-map-sequence-id-300582

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  31. pCS-H2B-mRFP1

    Plasmid
    #53745
    Purpose
    Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector
    Depositor
    Sean Megason
    Publication
    Megason et al Methods Mol Biol. 2009;546:317-32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone H2B (H2BC21 Human)
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53745-plasmid-map-sequence-id-242469

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  32. mKeima-Red-C1

    Plasmid
    #54546
    Purpose
    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKeima-Red
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54546-plasmid-map-sequence-id-256470

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Showing: 5041 - 5080 of 179521 results