164,577 results

1. MED12_pLX307

   Plasmid

   #98350

   Purpose

   Lentiviral expression of MED12

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   MED12 (MED12 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R1392Q

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSG5-BZLF1

   Plasmid

   #72637

   Purpose

   Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BZLF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q163R

   Promoter

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pUC18-mini-Tn7T-Gm-lux

   Plasmid

   #64963

   Purpose

   mini-Tn7 luxCDABE transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   lux operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. GFP-Dcp1a

   Plasmid

   #153972

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-ASAP5-Kv2.1-WPRE

   Plasmid

   #225707

   Purpose

   Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Has Service

   AAV1

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human synapsin

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-tdTomato

   Plasmid

   #28306

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMGF196

   Plasmid

   #97005

   Purpose

   Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   LEMD2 (LEMD2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV delta 5

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC-CTHF

   Plasmid

   #26105

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   TEV - His6 - Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-mRFP

   Plasmid

   #13032

   Purpose

   Mammalian expression vector for cloning your gene fused to mRFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Monomer Red Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-HA-PD-L1

    Plasmid

    #121492

    Purpose

    Expression of human PD-L1 WT in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 HA Arf6

    Plasmid

    #10834

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Rogi1-CMV-LAMB3-T2A-GFP-bGH

    Plasmid

    #179864

    Purpose

    Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

    Depositor

    George Church

    Article

    Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

    Insert

    LAMB3-T2A-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211463

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-dead
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-FOXA1

    Plasmid

    #153109

    Purpose

    Expression of Flag tagged human FOXA1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXA1 (FOXA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG_smFP V5

    Plasmid

    #59758

    Purpose

    encodes multiple copies of an epitope tag V5

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _V5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MaLionB

    Plasmid

    #113910

    Purpose

    Blue ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiV_Neo_LKB1

    Plasmid

    #108111

    Purpose

    Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    LKB1 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLSSmKate2-N1

    Plasmid

    #31867

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-DIO-EGFP-CAAX

    Plasmid

    #208685

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209725

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CPSF1

    Plasmid

    #155475

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF1 (CPSF1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSIH1-puro-control shRNA

    Plasmid

    #26597

    Purpose

    Lentiviral control shRNA vector with puromycin selection.

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJR101

    Plasmid

    #187241

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-NSP4SA11

    Plasmid

    #89171

    Purpose

    Express Rotavirus SA11 NSP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Antares2 c-myc

    Plasmid

    #100027

    Purpose

    A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    Antares2
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT7-VP2SA11

    Plasmid

    #89163

    Purpose

    Express Rotavirus SA11 VP2 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #126216

    Purpose

    Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HDAC1 Flag

    Plasmid

    #13820

    Purpose

    Mammalian expression of human histone deacetylase 1 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT7-NSP3SA11

    Plasmid

    #89170

    Purpose

    Express Rotavirus SA11 NSP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT7-VP4SA11

    Plasmid

    #89165

    Purpose

    Express Rotavirus SA11 VP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge