175,586 results

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pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam APC 1-1309

Plasmid

#16509

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 1309 truncation

Available Since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3/HA-FLAG RBP2 H483A

Plasmid

#14801

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Changed His 483 to Ala, R46W

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV-FOXP3-IRES-Thy1.1

Plasmid

#17443

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad3

Plasmid

#14052

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
FG12

Plasmid

#14884

Depositor

David Baltimore

Article

Qin et al Proc Natl Acad Sci U S A. 2003 Jan 7. 10

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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Hmga2 3'UTR m7 luciferase (Luc-m7)

Plasmid

#14788

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m7 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m7 mutant (see article and author's map for …

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1a2

Plasmid

#13751

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a2 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pRIAS mBmp4 (CT#578)

Plasmid

#14001

Depositor

Cliff Tabin

Insert

Bmp4 (Bmp4 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBSSK p85 alpha (delta iSH2)

Plasmid

#13432

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta iSH2) (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Mutation

Dominant negative p85 alpha. The p110 alpha bindi…

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pKM201

Plasmid

#13078

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Ptac-gam-bet-exo operon

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1-14-3-3 epsilon GST

Plasmid

#13279

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 epsilon (Ywhae Rat)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV HDM2(C464A)

Plasmid

#12086

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Expression

Mammalian

Mutation

C464A: RING-finger-domain mutant

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-HcRed

Plasmid

#11152

Purpose

Mammalian expression vector for expression of HcRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

HcRed1 from Heteractis crispa

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAP

Plasmid

#8340

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pJRH-1187 VSVGmut

Plasmid

#201913

Purpose

Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

VSV-G (K47Q, R354A)

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGD-p19

Plasmid

#196326

Purpose

35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tomato bushy stunt virus p19

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163056

Purpose

mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuide-zeo

Plasmid

#160091

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4-DdCBE- left side TALE

Plasmid

#157844

Purpose

expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Expression

Bacterial

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT077

Plasmid

#137879

Purpose

Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd (RESCUE)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI152-pLenti-SiT-Cas12a

Plasmid

#128405

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW SpCas9

Plasmid

#131506

Purpose

lentiviral expression of SpCas9 under hUBC promotor

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-MCS UBC promoter

Plasmid

#128045

Purpose

Lentiviral vector with UBC promoter

Depositor

Oskar Laur

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-TOMM20-N-10

Plasmid

#123284

Purpose

Localization: Outer Mitochondrial Membrane

Depositor

Kevin McGowan

Insert

TOMM20 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-PIK3CA

Plasmid

#116771

Purpose

Lentiviral expression of PIK3CA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-tTA

Plasmid

#104102

Purpose

Expresses tTA2 under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-CaaX(Hras)

Plasmid

#108886

Purpose

Expression of membrane localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry-CaaX(Hras)

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Expression

Mammalian

Promoter

7 repeats of Gli biding site

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde and AAV1

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-hnRNPA2_FL_WT

Plasmid

#98662

Purpose

Expresses MBP-tagged full length hnRNPA2

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MYC_pLX307

Plasmid

#98363

Purpose

Lentiviral expression of MYC

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

NA

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Article

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-hAsCpf1(TATV) (pY220)

Plasmid

#89353

Purpose

Expresses humanized AsCpf1 variant that recognizes TATV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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