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169,625 results

Showing: 7351 - 7400 of 169625 results

  6. pLKO-TRE-AFOS

    Plasmid
    #197079
    Purpose
    A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.
    Depositor
    Elaine Fuchs
    Article
    Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1
    Insert
    AFOS (Fos Mouse)
    Use
    Lentiviral
    Tags
    Flag and T7
    Promoter
    TRE
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197079-plasmid-map-sequence-id-396633

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  7. pcDNA3-BG4-NLS-mVen

    Plasmid
    #211449
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Tags
    NLS-mVenus
    Expression
    Mammalian
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211449-plasmid-map-sequence-id-419262

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  9. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Article
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available Since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    70110-plasmid-map-sequence-id-399654

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  10. pKL2299

    Plasmid
    #186332
    Purpose
    This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.
    Depositor
    Kan Wang
    Article
    Kang et al Front Plant Sci. 2022 May 4;13:860971. d
    Insert
    virulence genes from Agrobacterium tumefaciens pTiBo542
    Expression
    Bacterial
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    186332-plasmid-map-sequence-id-374264

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  12. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101849-plasmid-map-sequence-id-200249

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  14. DsRed2-Mito-7

    Plasmid
    #55838
    Purpose
    Localization: Mitochondria, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    DsRed2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55838-plasmid-map-sequence-id-244346

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  18. XLone-BSD SPI1 P2A mCherry

    Plasmid
    #140031
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140031-plasmid-map-sequence-id-275874

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  24. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227325-plasmid-map-sequence-id-452110

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  28. pHN764

    Plasmid
    #178819
    Purpose
    Expression of EndoH in E. coli
    Depositor
    Kazuhiro Abe
    Insert
    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    Tags
    HIs8 and MBP
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178819-plasmid-map-sequence-id-351934

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  34. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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  35. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  37. TFORF0384

    Plasmid
    #141599
    Purpose
    Lentiviral vector for overexpressing the SMARCA2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMARCA2 (SMARCA2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141599-plasmid-map-sequence-id-405434

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  44. pTP1122

    Plasmid
    #104159
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104159-plasmid-map-sequence-id-252709

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  46. mEmerald-Calreticulin-N-16

    Plasmid
    #54023
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54023-plasmid-map-sequence-id-474311

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  48. pCMV-SUN1 mScarlet

    Plasmid
    #187683
    Purpose
    In vivo visualization of SUN1
    Depositor
    Mary Beckerle
    Insert
    SUN1 (Sun1 Mouse)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187683-plasmid-map-sequence-id-371441

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  49. Dendra2-ER-5

    Plasmid
    #57716
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7351 - 7400 of 169625 results