162,377 results
-
Plasmid#188387PurposeLentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;DepositorInsertHNF1A-RE_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD4
Plasmid#33105DepositorInsertTEAD4 (TEAD4 Human)
UseTagsGal4ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
Plasmid#134859PurposeExpresses EGFP-tagged ERGIC markerDepositorInsertERGIC53 (Lman1 Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG1159 TOM20-ECFP-FIREmate
Plasmid#196693PurposeExpresses TOM20-ECFP-FIREmate in mammalian cellsDepositorInsertTOM20-ECFP-FIREmate
UseTagsFIREmateExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-eyfp
Plasmid#78466PurposeBroad range bacterial fluorescence labelingDepositorInserteYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-APEX2-NLS
Plasmid#140281PurposeExpression of APEX2-NLSDepositorInsertAPEX2-NLS
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-LaminA
Plasmid#69059Purposeencodes the lamin A proteinDepositorInsertLaminA (LMNA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p_m16S
Plasmid#160549PurposeUsed for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: CarbenicillineDepositorInsert16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ZO1-C-14
Plasmid#55165PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorInsertZO1 (TJP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti guide Thy1.1
Plasmid#128063PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LRCherry2.1
Plasmid#108099PurposeLentiviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGW1-Myc-DJ1-WT
Plasmid#29347DepositorInsertDJ1 (PARK7 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE
Plasmid#179256PurposeAAV-mediated expression of GCaMP8s under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSCMV:tdTomato
Plasmid#30530Purposemammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.DepositorHas ServiceCloning Grade DNAInserttdTomato
UseTagsExpressionMammalianMutationR415HPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PTP1B human
Plasmid#102719Purposehuman PTP1B catalytic domain (residues 1-301) in RP1B vectorDepositorInsertProtein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shNTC
Plasmid#217638PurposeDox inducible shRNA non targeting control shRNADepositorInsertshNTC
UseRNAiTagsExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1
Plasmid#207108PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 in human cells.DepositorInsertHaloTag-MDC1
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
tTA_BFP
Plasmid#58854PurposeConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)DepositorInserttTA transcription factor fused to enhanced blue fluorescent protein
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin2a KI
Plasmid#131486PurposeEndogenous tagging of GluN2a: N-terminal (amino acid position: A25)DepositorInsertgRNA and GFP donor (Grin2a Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-RH▲-MNase
Plasmid#136292PurposeExpression of catalytic inactive RNase H fused to micrococcal nucleaseDepositorInsertRNase HI-D10R-E48R and MNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIVT-mGL
Plasmid#232768PurposeIVT template for making modRNA encoding mGreenLanternDepositorInsertmGreenLantern
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-puro
Plasmid#17923PurposeInsect expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPP085
Plasmid#158795PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-pBAD
Plasmid#54542PurposeLocalization: Protein Expression Vector , Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTags6xHis and EBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAM_Cam
Plasmid#91570PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB24 pDEST crybb1:TagBFP
Plasmid#195987PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-SUMO2-Q90P
Plasmid#36448PurposeExpresses HA-Sumo2Q90P mutant in mammalian cellsDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHAExpressionMammalianMutationMutated Q90PPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWL078 MCP-2xGFP
Plasmid#197047PurposepCYC-NLS-MCP-2xGFP on pRS305DepositorInsertMCP-2xGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV-ABEmax-NRTH
Plasmid#136922PurposeExpresses ABEmax-NRTH in mammalian cellsDepositorInsertABEmax-NRTH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vimentin-7
Plasmid#55156PurposeLocalization: Intermediate Filaments, Excitation: 587, Emission: 610DepositorInsertVimentin (VIM Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUAL SRBI (GFP)
Plasmid#86979PurposeLentiviral expression construct encoding SR-B1 and GFP from separate promotersDepositorInsertSCARB1 (SCARB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
GST-MNase
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mouse E-cadherin GFP (423)
Plasmid#67937PurposeMammalian expression of E-cadherin-GFPDepositorInsertE-cadherin (Cdh1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Nesprin-1αKASH
Plasmid#187015PurposeDisruption of LINC ComplexDepositorInsertNesprin-1alphaKASH
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Parkin
Plasmid#17613DepositorInsertParkin (PRKN Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42(Q61L)
Plasmid#12600DepositorInsertEGFP-Cdc42 (Q61L) (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61LPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tailed mCherry-SEpHluorin
Plasmid#32002DepositorInsertMembrane-targeting sequence of Lyn kinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-3xFLAG
Plasmid#69837PurposeThis plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-Nanoluc
Plasmid#87075PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorInsertp16-INK4A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE Tet ONAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-puro
Plasmid#45567DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsIRES-EGFP-PuroExpressionMammalianMutationPromoterPhcmv (strong human cytomegalovirus immediate ear…Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti sgRNA(MS2) mCherry
Plasmid#171053PurposeExpression of Vp64-dCas9 activation systemDepositorInsertsgRNA(MS2) mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-p53 (1-90) (72R)
Plasmid#62081PurposeExpression of human p53 (TAD residues 1-90) (72R) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains TAD/PP residues 1-90, 72RPromotertacAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only