9,049 results

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pcDNA-flag-mKdm4d-polyA

Plasmid

#61553

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (Kdm4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-C1

Plasmid

#54613

Purpose

Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEBG-Pak1 83-149

Plasmid

#12214

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Mammalian

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pQLinkG2

Plasmid

#13671

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

GST, TEV, and attB1

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pJBEI-6411

Plasmid

#47050

Purpose

BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase

Use

Synthetic Biology; Bglbrick

Expression

Bacterial

Promoter

Pbad

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242)

Plasmid

#18874

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pMXs-OLIG2

Plasmid

#32933

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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mTLR2

Plasmid

#13083

Depositor

Ruslan Medzhitov

Insert

TLR2 (Tlr2 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 wt (Nigg RG6)

Plasmid

#41160

Depositor

Erich Nigg

Article

Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP.N1-HDAC6.DC (catalytic deficient)

Plasmid

#36189

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

HDAC6.DC (HDAC6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H216/611A

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Artemis

Plasmid

#40211

Purpose

Mammalian expression of codon-optimized Artemis.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Artemis (DCLRE1C Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-AXL

Plasmid

#23945

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AXL (AXL Human)

Use

Gateway donor vector

Mutation

Q31P, Q764R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCINeo-myc-Itch (C822S)/2860

Plasmid

#11428

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Tags

myc

Expression

Mammalian

Mutation

cysteine at 822 mutated to serine

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-FRT/T0-Flag-BRCA1

Plasmid

#71117

Purpose

mammalian expression of Flag-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV/TO

Available Since

Nov. 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

Plasmid

#64221

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe puro Snail

Plasmid

#23347

Depositor

Bob Weinberg

Article

Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

Insert

Snail (SNAI1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-CIBN-CreC

Plasmid

#26889

Purpose

Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN-CreC

Expression

Mammalian

Mutation

aa 1-170 of CIB1, aa 106-343 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGolden-Neo

Plasmid

#51422

Purpose

Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

PGK1 phosphoglycerate kinase 1 (PGK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pWZL Neo Myr Flag PTK2

Plasmid

#20610

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PTK2 (PTK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLM292

Plasmid

#21873

Depositor

Keith Joung

Article

Foley et al Nat Protoc. 2009. 4(12):1855-67.

Type

Empty backbone

Use

Zebrafish expression

Tags

Flag

Expression

Mammalian

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLM290

Plasmid

#21872

Depositor

Keith Joung

Article

Foley et al Nat Protoc. 2009. 4(12):1855-67.

Type

Empty backbone

Use

Zebrafish expression

Tags

Flag

Expression

Mammalian

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-O-FUW-Sox10

Plasmid

#45843

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS2991

Plasmid

#8931

Depositor

Pamela Silver

Article

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Insert

NDR2 (STK38L Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
SRa-HA-SUMO2 (Sentrin 3)

Plasmid

#17360

Purpose

Mammalian expression of human SUMO2 with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 NSP9

Plasmid

#141263

Purpose

Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAd-Wnt3a

Plasmid

#12518

Depositor

Tong-Chuan He

Article

Luo et al J Biol Chem. 2004 Dec 31. 279(53):55958-

Insert

Wnt3A (Wnt3a Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only
mKO2-C1

Plasmid

#54494

Purpose

Localization: C1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-CG

Plasmid

#12154

Depositor

Inder Verma

Article

Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

Insert

CMV-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Au1-mTOR-Wild type

Plasmid

#26036

Depositor

Fuyuhiko Tamanoi

Article

Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

Insert

mammalian target of rapamycin-wild type (Mtor Rat)

Tags

AU1

Expression

Mammalian

Available Since

Sept. 21, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HK2

Plasmid

#20501

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK2 (HK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-syn-jGCaMP7b-WPRE

Plasmid

#104489

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV Retrograde and AAV1

Insert

jGCaMP7b

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-Ef1a-mCh

Plasmid

#31847

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 ORF7B

Plasmid

#141277

Purpose

Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGH153_MS2-AIDΔ-Hygro

Plasmid

#85416

Purpose

lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pNW55

Plasmid

#22988

Purpose

Expression of miRNA precursor for the osa-MIR528 locus in rice.

Depositor

Detlef Weigel

Article

Warthmann et al PLoS One. 2008 . 3(3):e1829.

Insert

osa-MIR528

Use

RNAi; Plant gene inactivation

Expression

Plant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-K-RAS V12

Plasmid

#31200

Purpose

Gateway donor vector containing KRAS G12V.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway donor vector

Mutation

G12V constitutively active

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
IL13 promoter luciferase

Plasmid

#11784

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-13 promoter (IL13 Human)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
pLV.ATMi

Plasmid

#14542

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATM shRNA (ATM Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo beta catenin delta45

Plasmid

#16520

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Mutation

Delta 45 (HCT116 mutation)

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
903 pLNCX HA Akt1

Plasmid

#9004

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge

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9,049 results