159,352 results

1. pAAV_BiLAMP5e3_Gq_dTomato

   Plasmid

   #213916

   Purpose

   AAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   HA-GqDREADD-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MsACR1-mCerulean3-pAAV2-hSyn

   Plasmid

   #204965

   Purpose

   Panneuronal expression of MsACR1 fused to mCerulean3

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   MsACR1
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

   Plasmid

   #204970

   Purpose

   Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   MsACR1
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p8328 pLIX YAP1 S94A

   Plasmid

   #184529

   Purpose

   Expression of YAP1 S94A

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 S94A (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S94A

   Promoter

   TRE promoter, Tet ON

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSNAP-CLC-SNAP

   Plasmid

   #38012

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Use

   Tags

   SNAP26m

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. dSpCas9-VPR

   Plasmid

   #205247

   Purpose

   In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

   Plasmid

   #194683

   Purpose

   CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_05-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLEX-MCS PGK promoter

   Plasmid

   #128044

   Purpose

   Lentiviral vector with PGK promoter

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mVenus N1

   Plasmid

   #27793

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PGK mScarlet-Synaptophysin

    Plasmid

    #206145

    Purpose

    PGK mScarlet-Synaptophysin

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    synaptophysin (Syp Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131003

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-IRES-GFP-mouse-Caspase-1

    Plasmid

    #183361

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Use

    Tags

    EGFP and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECE-M2-PAK2 wt

    Plasmid

    #31663

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENN.AAV.EF1a.eGFP.WPRE.rBG

    Plasmid

    #105547

    Purpose

    AAV expression of EGFP from EF1a promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC-LYCipi

    Plasmid

    #53279

    Purpose

    Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28sumo-hRig-I-Full-length

    Plasmid

    #210827

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

    Insert

    hRIG-I (DDX58) Full length protein (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE2-TetOminiCMV-OSK

    Plasmid

    #136552

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXs-hs-EGR1

    Plasmid

    #52724

    Purpose

    retroviral expression of human EGR1

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p-LCN2

    Plasmid

    #28225

    Depositor

    Sebastien Jauliac

    Article

    Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

    Insert

    LCN2 promoter (LCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    LCN2

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

26. mScarlet-Rab9a

    Plasmid

    #169074

    Purpose

    Express human Rab9a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab9a (RAB9A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. miReporter-PGK

    Plasmid

    #82477

    Purpose

    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSB-BbsI-sgRNA

    Plasmid

    #203359

    Purpose

    Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    BbsI Cut Sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Plasmid

    #100798

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    TVA, EGFP, tet transactivator
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

    Plasmid

    #174876

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser2ph mintbody-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-MAP3K5 (WT)

    Plasmid

    #47104

    Purpose

    Expresses wild type human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGFPHYG2

    Plasmid

    #30173

    Depositor

    Lalita Ramakrishnan

    Article

    Cosma et al Infect Immun. 2006 Jun . 74(6):3125-33.

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    GFP

    Expression

    Mutation

    GFPmut3 allele has improved brightness.

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRU44

    Plasmid

    #167666

    Purpose

    ENTRY vector for gRNA4 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKM464 (deletions)

    Plasmid

    #108322

    Purpose

    Orbit integrating plasmid to delete target gene.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integration plasmid for creating knockouts …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220735

    Purpose

    AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-VEGFR2-mCherry

    Plasmid

    #139606

    Purpose

    Expresses a VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSF-G1TFAKRS

    Plasmid

    #177311

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PBAD-his6-prkA-pACYC184

    Plasmid

    #41041

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    prkA
    

    Use

    Tags

    6xHis and T7 epitope tag

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDawn-sfGFP

    Plasmid

    #107741

    Purpose

    Expresses superfolder GFP under the control of a blue-light transcriptional regulator

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLambda

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Beta1-GFP in pHcgreen

    Plasmid

    #69804

    Purpose

    Expresses Beta1-GFP integrin for normal cell transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1A integrin (ITGB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. CRY2 mCherry Sec61B

    Plasmid

    #102246

    Purpose

    Expresses fusion of CRY2PHR with mCherry and Sec61B

    Depositor

    Bianxiao Cui

    Article

    Che et al ACS Synth Biol. 2015 Oct 16;4(10):1124-3

    Insert

    CRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

    Plasmid

    #226514

    Purpose

    Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

    Depositor

    Kai Johnsson

    Article

    Kühn et al bioRxiv 2024.08.28.610127

    Insert

    HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

    Plasmid

    #194680

    Purpose

    CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Hpep1-betaArrestin2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLSLGFP

    Plasmid

    #51494

    Purpose

    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GFP
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. Beta-2-adrenergic receptor-YFP

    Plasmid

    #67146

    Purpose

    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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