20,109 results

Showing: 21 - 40 of 20109 results

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pEGFP-N1-RACK1

Plasmid

#41088

Depositor

Anna Huttenlocher

Article

Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Insert

Receptor for activated C kinase (RACK1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pICH47761

Plasmid

#48003

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

Plasmid

#113899

Purpose

Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

TetR-HA-NLS-P2A-BSD-Myc

Use

Lentiviral

Tags

HA-Myc

Promoter

CMV-MIE

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-PLE-IRES-mCherry

Plasmid

#45766

Depositor

Luke Lavis

Article

Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

Insert

esterase

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pICH47772

Plasmid

#48004

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Rac1

Plasmid

#180576

Purpose

ddRFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Rac1

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP

Plasmid

#21037

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Tags

mEPFP

Expression

Bacterial and Mammalian

Available Since

May 12, 2009

Availability

Academic Institutions and Nonprofits only

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 SIRT1-FL

Plasmid

#105670

Purpose

Mammalian expression construct of mouse SIRT1 full length isoform

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRSV-Rev

Plasmid

#12253

Purpose

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Article

Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Insert

Rev (rev )

Use

Lentiviral; Packaging

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pMDLg/pRRE

Plasmid

#12251

Purpose

3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Article

Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Insert

HIV-1 GAG/POL

Use

Lentiviral; Packaging

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP (PX458)

Plasmid

#48138

Purpose

Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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CRISPRoff-v2.1

Plasmid

#167981

Purpose

Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)

Tags

2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3_C1

Plasmid

#189753

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2/9n

Plasmid

#112865

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap9

Use

AAV

Promoter

Truncated P5

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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