19,750 results

1. pCMV-PEmax

   Plasmid

   #174820

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-mt-mKeima

   Plasmid

   #131626

   Purpose

   lentiviral expression of mitochondrial mKeima for generation of stable cell lines

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPgRNA

   Plasmid

   #47108

   Purpose

   Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   SPgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP

   Plasmid

   #13031

   Purpose

   Mammalian expression vector for cloning your gene fused to EGFP

   Depositor

   Doug Golenbock

   Insert

   Enhanced Green Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-hTERT-IRES-hygro

   Plasmid

   #85140

   Purpose

   Lentiviral expression of hTERT, used to create immortalized cell lines

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-ATLASsnCre

   Plasmid

   #232351

   Purpose

   Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Has Service

   AAV5

   Insert

   ATLASsnCre
   

   Use

   AAV

   Tags

   ALFA-Tag

   Promoter

   hSyn

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX_307

   Plasmid

   #41392

   Purpose

   Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX313-Firefly luciferase

   Plasmid

   #118017

   Purpose

   Constitutive expression of Firefly luciferase

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-VEGFR1-V5/HIS

   Plasmid

   #205616

   Purpose

   Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR1 receptor tyrosine kinase (FLT1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti PGK Puro DEST (w529-2)

    Plasmid

    #19068

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205550

    Purpose

    Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSGV-1D3-28Z All ITAMs intact

    Plasmid

    #107226

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs intact

    Depositor

    James Kochenderfer, Steven Rosenberg

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z All ITAMs intact
    

    Use

    Retroviral

    Tags

    None

    Mutation

    None

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-UBC-gate-3xHA-pGK-PUR

    Plasmid

    #107393

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV R-CEPIA3mt

    Plasmid

    #140464

    Purpose

    Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

    Insert

    R-CEPIA3mt
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-Flp

    Plasmid

    #122969

    Purpose

    Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

    Depositor

    Lydia Freddolino

    Article

    Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

    Insert

    Flp recombinase
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. EZ-Tet-pLKO-Blast

    Plasmid

    #85973

    Purpose

    Tet/Dox inducible shRNA lentivirus with blasticidin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Promoter

    H1 / TetO

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC5-Tmem192-3xHA

    Plasmid

    #102930

    Purpose

    Lentiviral expression of a lysosomal tag (Tmem192-HA)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    Tmem192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid

    #26429

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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