165,271 results

1. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mNeonGreen-Sec61β

   Plasmid

   #169031

   Purpose

   General ER marker

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Sec61β (SEC61B Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9-2A-Cre

   Plasmid

   #68468

   Purpose

   co-expressing Cas9 and Cre recombinase

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   Cas9 and Cre recombinase
   

   Use

   CRISPR

   Tags

   P2A-Cre-Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKII-pAce-Kv2.1PR

   Plasmid

   #195529

   Purpose

   Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CaMKII

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAPB02

   Plasmid

   #65628

   Purpose

   A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

   Depositor

   Pei-yuan Qian

   Article

   Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF-G1TFAKRS

   Plasmid

   #177311

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PEmax

   Plasmid

   #174820

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJiaDEST-APEX2-GAL3

   Plasmid

   #149518

   Purpose

   Express APEX2-Galectin-3 in mammalian cells

   Depositor

   Vojo Deretic

   Article

   Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

   Insert

   LGALS3 (LGALS3 Human)
   

   Tags

   APEX2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDest-311 (attR4-attR3)

    Plasmid

    #161896

    Purpose

    Multisite Gateway (attR4-attR3) vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT204

    Plasmid

    #134881

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Tags

    KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-BTK-mCherry

    Plasmid

    #50043

    Purpose

    Expression of BTK-mCherry in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BTK (Btk Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGreen_dualluc_ORF_sensor

    Plasmid

    #55207

    Purpose

    Dual-luciferase reporter for microRNA targeting in plants

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Luciferase; Plant transient expression

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. dpYPQ230-D156R-CBE4

    Plasmid

    #195369

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…

    Expression

    Plant

    Promoter

    ZmUBI1

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hCD4-mOrange

    Plasmid

    #110192

    Purpose

    Encodes for human CD4 coding sequence tagged with the mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCD4 (CD4 Human)
    

    Tags

    mOrange

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. TDP-43 WT GFP

    Plasmid

    #237542

    Purpose

    Expression of GFP-tag fused to wild-type TDP-43

    Depositor

    Tatyana Shelkovnikova

    Article

    Matsukawa et al RNA Biol. 2021 Jan 11:1-9. doi: 10.1080/

    Insert

    GFP-tagged TDP-43 WT (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild-type TDP-43

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTP223

    Plasmid

    #13263

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    Plac-gam-bet-exo
    

    Expression

    Bacterial

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid

    #87803

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid

    #170273

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    See Comments

    Promoter

    CMVd1

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCIneoGFP-BMI1

    Plasmid

    #128328

    Purpose

    Mammalian expression vector of GFP-tagged human BMI1

    Depositor

    Yutaka Hata

    Article

    Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

    Insert

    BMI1 (BMI1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDest-676 (attR4-attR3)

    Plasmid

    #161888

    Purpose

    Multisite Gateway (attR4-attR3) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

    Plasmid

    #169449

    Purpose

    expression of TRF1-mcherrry and sgRNA targeting telomeres

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    mCherry-TRF1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid

    #44095

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    3xFlag, 6xGly linker, ADH1 transcription terminat…

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GSN-bio-His

    Plasmid

    #52067

    Purpose

    Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GSN (GSN Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. V72 ELP

    Plasmid

    #68938

    Purpose

    Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

    Depositor

    Andrew MacKay

    Article

    Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

    Insert

    V72 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. IFI16-FL-(1-729)

    Plasmid

    #35051

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16 (IFI16 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid

    #162877

    Purpose

    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Insert consists of AA1-170

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS

    Plasmid

    #12158

    Depositor

    Inder Verma

    Article

    Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAT206

    Plasmid

    #134883

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJJiaDEST-APEX2-GAL9

    Plasmid

    #149520

    Purpose

    Express APEX2-Galectin-9 in mammalian cells

    Depositor

    Vojo Deretic

    Article

    Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

    Insert

    LGALS9 (LGALS9 Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYPQ-CBE-SpRYn-Act3.0

    Plasmid

    #178958

    Purpose

    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pMRE-Tn7-143

    Plasmid

    #118559

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Tubp-Gal4 in pCaSpeR4

    Plasmid

    #17747

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Tags

    Tubulin 1alpha promoter and hsp70 PolyA

    Expression

    Insect

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Tags

    3x-Flag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Promoter

    Chicken beta actin (CAG)

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHiUGE smFP-V5 donor (all-in-one) ORF0

    Plasmid

    #200386

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. tdTomato-Integrin-Beta1-N-18

    Plasmid

    #58105

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

44. pHCMV_JEV_prME

    Plasmid

    #207283

    Purpose

    Mammalian expression of Japanese encephalitis virus PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    JEV_PrME (POLY Japanese encephalitis virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHAGE-RET

    Plasmid

    #116787

    Purpose

    Lentiviral expression of RET

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RET (RET Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLenti-nSpCas9-APOBEC3A

    Plasmid

    #209040

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC3A-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pHiUGE smFP-V5 donor (all-in-one) ORF1

    Plasmid

    #200387

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. p2CT-His-MBP-Hhe_Cas13a_WT

    Plasmid

    #91871

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    HheCas13a (HheC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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