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169,486 results

Showing: 951 - 1000 of 169486 results

  2. pX461-ABE8e-nSpCas9

    Plasmid
    #237460
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun 17, 1218 (2026)
    Insert
    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237460-plasmid-map-sequence-id-476159

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  3. pX461-evoCDA1-nSpCas9

    Plasmid
    #237461
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun 17, 1218 (2026)
    Insert
    3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237461-plasmid-map-sequence-id-476160

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  4. pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

    Plasmid
    #249156
    Purpose
    Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249156-plasmid-map-sequence-id-496442

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  5. pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249160
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Human, Synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249160-plasmid-map-sequence-id-496435

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  10. CRBN-NanoLuc Vector

    Plasmid
    #238749
    Purpose
    Express CRBN-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CRBN (CRBN Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238749-plasmid-map-sequence-id-481948

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  13. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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  14. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  18. pXR004_puroR

    Plasmid
    #219820
    Purpose
    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219820-plasmid-map-sequence-id-441123

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  20. TFORF0450

    Plasmid
    #143702
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DNMT1 (DNMT1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143702-plasmid-map-sequence-id-409075

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  21. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  22. pAAV-sCAG-GluClα.CreON

    Plasmid
    #196995
    Purpose
    Cre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.
    Depositor
    David Bennett
    Article
    Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0
    Insert
    GluClα -Cerulean
    Use
    AAV and Cre/Lox
    Tags
    Cerulean
    Promoter
    short CAG
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196995-plasmid-map-sequence-id-390150

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  23. pGA-blue

    Plasmid
    #196336
    Purpose
    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196336-plasmid-map-sequence-id-393157

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  25. T7-TetO-GFP_ColE1

    Plasmid
    #173193
    Purpose
    Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    T7-TetO
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173193-plasmid-map-sequence-id-339027

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  27. pTJK438

    Plasmid
    #138004
    Purpose
    3xMYC-SIX1 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    3xMYC tagged SIX1 (SIX1 Human)
    Use
    Lentiviral
    Tags
    3xMYC
    Mutation
    None
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138004-plasmid-map-sequence-id-274998

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  37. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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  43. pAAV2/8

    Plasmid
    #112864
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap8
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112864-plasmid-map-sequence-id-333531

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  46. pAAV2/9n

    Plasmid
    #112865
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap9
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112865-plasmid-map-sequence-id-350300

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Showing: 951 - 1000 of 169486 results