We narrowed to 18,870 results for: IRE

1. pMXs-OLIG2

   Plasmid

   #134668

   Purpose

   Retroviral expression of human OLIG2

   Depositor

   Marius Wernig

   Article

   Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

   Insert

   OLIG2 (OLIG2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Ngn2

   Plasmid

   #34999

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Neurogenin-2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

   Plasmid

   #226921

   Purpose

   Encoding SauriABE8e and sgRNA cassette on a single AAV

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   TadA8e, nSauriCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGH020_sgRNA_G418-GFP

   Plasmid

   #85405

   Purpose

   hu6 driven sgRNA vector with G418 and GFP selectable markers

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   G418 resistance and GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUW-tetO-hKLF4

   Plasmid

   #20725

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVEX-CC1

   Plasmid

   #74170

   Purpose

   protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

   Depositor

   Trina Schroer

   Article

   Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

   Insert

   DCTN1 (DCTN1 Chicken)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Inducible Tac promoter

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pX330-SpCas9-HF1

   Plasmid

   #108301

   Purpose

   pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. rAAV2/cMG.QRP

   Plasmid

   #184543

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-cMG.QRP capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-cMG.QRP capsid
   

   Use

   AAV; Helper vector for packaging

   Tags

   Expression

   Mutation

   changes on AAV9 capsid: K449R; insertion of QRPPR…

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

    Plasmid

    #191621

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME18s-HA-hFIP200

    Plasmid

    #24303

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDestattB/CY (pCM327)

    Plasmid

    #68341

    Purpose

    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330-HypaCas9

    Plasmid

    #108302

    Purpose

    pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMB-SaCas9

    Plasmid

    #105998

    Purpose

    SaCas9 expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Insert

    Cas9 nuclease
    

    Use

    CRISPR and Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    short EF1alpha

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-CAG-DIO-APEX2NES

    Plasmid

    #79907

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    APEX2NES
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-GFAP104-melanopsin-mCherry

    Plasmid

    #122630

    Purpose

    AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

    Depositor

    Edward Boyden

    Article

    Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

    Insert

    melanopsin-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP104

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

    Plasmid

    #135455

    Purpose

    Bacterial expression of uTEV1Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, S153N mutation…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

    Plasmid

    #62951

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR- flag-Lag17-iCAM-1

    Plasmid

    #205204

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag17 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTLR repair vector

    Plasmid

    #64322

    Purpose

    Vector for repair of the traffic light reporter (TLR), providing the venus coding region

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    Venus
    

    Use

    Artificial targeting vector

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    none

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+)-wt-TTF-1

    Plasmid

    #49989

    Purpose

    For transient transfection to express human wild type TTF-1 protein

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    TTF-1 (NKX2-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3/mArf1(Q71L)-HA

    Plasmid

    #79410

    Purpose

    Expresses C-terminally HA-tagged Arf1(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf1 (Arf1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 71 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMXs-SOX10

    Plasmid

    #134669

    Purpose

    Retroviral expression of human SOX10

    Depositor

    Marius Wernig

    Article

    Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

    Insert

    SRY-box transcription factor 10 (SOX10 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Wild-type AdPol vector

    Plasmid

    #131685

    Purpose

    A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus DNA polymerase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-CAG::Cytbow

    Plasmid

    #158995

    Purpose

    Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Cytbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. XNM-Luc

    Plasmid

    #35157

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 152 bp of the myc promoter

    Promoter

    c-myc

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

29. tetO-NKX2.5

    Plasmid

    #46029

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    NKX2.5 (NKX2-5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. C1-F-tractin-mCitrine

    Plasmid

    #155217

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7-EGFP-C1-HsNot8

    Plasmid

    #37367

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot8 (CNOT8 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EF1a_TBX5_P2A_Hygro_Barcode

    Plasmid

    #120486

    Purpose

    Barcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

    Plasmid

    #62956

    Purpose

    Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C3 luc (T1 del)

    Plasmid

    #11358

    Depositor

    Donald McDonnell

    Article

    Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

    Insert

    C3 promoter (C3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFUW-tetO-mCherry

    Plasmid

    #193060

    Purpose

    doxycycline-inducible expression of mCherry in mammalian cell

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. myc-mTOR (1750-2549 aa)

    Plasmid

    #21747

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1750-2549) (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1750-2549

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pMXs-GLIS1

    Plasmid

    #30166

    Purpose

    Retroviral expression of non-canonical human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Maekawa et al Nature. 2011 . 474(7350):225-9.

    Insert

    GLIS1 (GLIS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3/mArf1(T31N)-HA

    Plasmid

    #79411

    Purpose

    Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf1 (Arf1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LentiCRISPRv2-Beclin1

    Plasmid

    #99574

    Purpose

    Expressed Cas9 with sgRNA targeting Beclin1

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting Beclin1 (BECN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge