We narrowed to 18,645 results for: Met
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Plasmid#158364PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
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GST-MAGE-2-429
Plasmid#89129Purposebacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sitesDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorTagsGST and His6ExpressionBacterialPromoterT7Available SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-yibDp-Erv1p
Plasmid#202483PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9
Plasmid#169334PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
TagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP5
Plasmid#158356PurposeMAC-tagged gene expressionDepositorAvailable SinceNov. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 S
Plasmid#158371PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
(326) pAAV SV40-ZsGreen U6-gRNA
Plasmid#163020PurposeFluorescent reporter for Sa gRNA (Bsa1 sites)DepositorInsertZs Green
UseAAVExpressionMammalianPromoterSV40Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-Luc
Plasmid#122694PurposeOutput expression of firefly luciferase under the activation of BenR transcription factor for cell-free expressionDepositorInsertFirefly luciferase
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TMPRSS3
Plasmid#221298PurposeExpression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
mADAM17deltaMP in pQCXIP cFLAG
Plasmid#19144DepositorAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateMutationThe 3’-end of the chloramphenicol resistance gene…Available SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY012 (pcDNA3.1-hCMtCpf1)
Plasmid#69984PurposeExpresses humanized CMtCpf1DepositorInserthCMtCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Src-Rescue
Plasmid#26983DepositorAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS84, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pzyxin-emiRFP703
Plasmid#136568PurposeZyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertzyxin-emiRFP703
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 Orf6
Plasmid#158374PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUBQ10::ZF108_3xFLAG_YPet
Plasmid#106441PurposepUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsisDepositorInsertZF108_3xFLAG_YPet
UseSynthetic BiologyExpressionPlantAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 E
Plasmid#158368PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1-puro-PARG shRNA
Plasmid#23260DepositorInsertpoly(ADP-ribose) glycohydrolase shRNA
UseLentiviral and RNAiExpressionMammalianAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-34 MT
Plasmid#78259PurposepsiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated miR-34 target site
UseLuciferase; Microrna activityExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CCGT to GG…Available SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2
Plasmid#169531PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).DepositorInsertPCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-LacI-p300
Plasmid#179270PurposeExpresses GFP-LacI-p300 fusion plasmidDepositorAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
ExpressionBacterialPromoterlacAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES EGFP
Plasmid#130706PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES EGFP
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP1
Plasmid#158352PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AYBEv0.1
Plasmid#193966PurposeA wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(wt)
ExpressionMammalianPromoterhU6, EF1a, CBhAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS SK mCherryROSAbsr
Plasmid#54321PurposeEncodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.DepositorInsertmCherry and BlasticidinS-resistance
UseCRISPR, Mouse Targeting, and TALENExpressionMammalianPromoterCMVAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1ExpressionYeastAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisPromoterPBenAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorMutationR691A from wild-type Cas9PromoterT3Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alanineAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
Tags6xHisExpressionYeastMutationG951A,C957AAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisPromoterOR2-OR1-PrAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-lox-mCherry-hyBRET-ERK-NLS
Plasmid#170290PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.DepositorInsertlox-mCherry-lox-hyBRET-ERK-NLS
ExpressionMammalianAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP2
Plasmid#158353PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 Orf10
Plasmid#158380PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoter35SAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-NDUFB2
Plasmid#139841PurposeLentiviral expression of human NDUFB2DepositorInsertNDUFB2 (NDUFB2 Human)
UseLentiviralAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S::Cel1SP-GeNL/pCAMBIA1301
Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
ExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only