Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,170 results

Showing: 10751 - 10800 of 177170 results

  12. pcDNA5-FRT-TO-EGFP-AID

    Plasmid
    #80075
    Purpose
    Mammalian expression vector for N-terminal EGFP-AID tagging
    Depositor
    Andrew Holland
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-AID
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    80075-plasmid-map-sequence-id-150337

    Enlarge

  21. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

    Enlarge

  22. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70018-plasmid-map-sequence-id-124557

    Enlarge

  25. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

    Plasmid
    #69623
    Purpose
    A FRET sensor for Galphai1 activation, encoded on a single plasmid.
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gbeta-2A-cpV-Ggamma2
    Galphai-mTurquoise2
    Tags
    ciruclar permutated Venus (cp173V) and mTurquoise…
    Expression
    Mammalian
    Promoter
    CMV and CMV (IRES)
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    69623-plasmid-map-sequence-id-459280

    Enlarge

  29. GPER-Tango

    Plasmid
    #66299
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPER (GPER1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66299-plasmid-map-sequence-id-173899

    Enlarge

  30. ADRA2A-Tango

    Plasmid
    #66216
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADRA2A (ADRA2A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66216-plasmid-map-sequence-id-417865

    Enlarge

  36. pCMV-Tag4-OMA1

    Plasmid
    #61719
    Purpose
    mammalian expression of human Oma1 with C-terminal FLAG tag
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61719-plasmid-map-sequence-id-175330

    Enlarge

  38. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Publication
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65216-plasmid-map-sequence-id-115626

    Enlarge

  39. pHLSec2-FN-YPet

    Plasmid
    #65421
    Purpose
    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
    Depositor
    Harold Erickson
    Publication
    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    Tags
    YPet
    Expression
    Mammalian
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    65421-plasmid-map-sequence-id-115435

    Enlarge

  40. Str-KDEL_ManII-SBP-mCherry

    Plasmid
    #65253
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    Streptavidin Binding Protein (SBP) and mCherry
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65253-plasmid-map-sequence-id-110461

    Enlarge

  43. pcDNA3-Lyn-AKAR4

    Plasmid
    #61620
    Purpose
    Lipid raft-targeted FRET biosensor for monitoring PKA activity
    Depositor
    Jin Zhang
    Publication
    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-AKAR4
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    61620-plasmid-map-sequence-id-100421

    Enlarge

  44. pSBtet-GH

    Plasmid
    #60498
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    60498-plasmid-map-sequence-id-97549

    Enlarge

  45. pSBtet-GN

    Plasmid
    #60501
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    60501-plasmid-map-sequence-id-97546

    Enlarge

Showing: 10751 - 10800 of 177170 results