159,557 results

1. hKCNQ3(WT).ires.mCherry-pcDNA3

   Plasmid

   #204362

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ3 (KCNQ3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-CMV-Pink Flamindo-p2a-bPAC

   Plasmid

   #202716

   Purpose

   Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tet-pLKO-FOXM1-shRNA-#2

   Plasmid

   #89499

   Purpose

   Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV hUbC-dCas9 KRAB-T2A-GFP

   Plasmid

   #67620

   Purpose

   Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 KRAB T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY108 (lenti-AsCpf1)

   Plasmid

   #84739

   Purpose

   Lenti virus delivery of AsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330 spCas9-mSA

   Plasmid

   #113096

   Purpose

   pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   Synthetic Biology

   Tags

   MSA (monomeric streptavidin

   Expression

   Mutation

   none

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCh-Rab7A

    Plasmid

    #61804

    Purpose

    Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG-SENP2

    Plasmid

    #18047

    Depositor

    Edward Yeh

    Article

    Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHCMV-RabiesG

    Plasmid

    #15785

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 HA 14-3-3 sigma

    Plasmid

    #11946

    Depositor

    Michael Yaffe

    Article

    Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

    Insert

    Stratifin (SFN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tdTomato-CD9-10

    Plasmid

    #58076

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

    Plasmid

    #216696

    Purpose

    Synchronize trafficking of TNFalpha from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    Tumor Necrosis Factor alpha (TNFa)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-BICDN-CIBN

    Plasmid

    #102254

    Purpose

    Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    GFP-BICDN-CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SMARCA2-GFP

    Plasmid

    #65390

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA2 (SMARCA2 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221_SLCO1B1

    Plasmid

    #132188

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO1B1 (SLCO1B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pMEH343 CD63 MS2 HA

    Plasmid

    #168217

    Purpose

    Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA and MS2 dimer

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMTTH+H1.4

    Plasmid

    #157796

    Purpose

    Expression of human linker histone H1.4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4 (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF2314

    Plasmid

    #142029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RXRB (RXRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CLYBL-TO-hNIL-BSD-mApple

    Plasmid

    #124230

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Insert

    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDR196

    Plasmid

    #36029

    Depositor

    Dominique Loque, Wolf Frommer, Doris Rentsch

    Article

    Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV8-hSyn-flex-miR30-eGFP-shDyn

    Plasmid

    #132716

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphin

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shDyn (Pdyn Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin 1

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AICSDP-166:CDH1-mEGFP

    Plasmid

    #193921

    Purpose

    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

    Depositor

    Allen Institute for Cell Science

    Insert

    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    

    Use

    AAV and CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pTRIP-SFFV-GFP-RELA

    Plasmid

    #196629

    Purpose

    Express GFP-RELA

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    GFP-RELA (RELA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMVHA EED wt

    Plasmid

    #24231

    Purpose

    Mammalian expression of human EED with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EED (EED Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Anti-GST [N100/13R ]

    Plasmid

    #188214

    Purpose

    Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC gRNA Shuttle

    Plasmid

    #47024

    Purpose

    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Insert

    gRNA Shuttle
    

    Use

    CRISPR; Cas9

    Tags

    Expression

    Plant

    Mutation

    G to T cloning mutation at position 323

    Promoter

    Medicago truncatula U6.6

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNAI Neo mouse PECAM FL

    Plasmid

    #42901

    Depositor

    Steven Albelda

    Insert

    PECAM (Pecam1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

36. Golgi-TurboID

    Plasmid

    #200959

    Purpose

    Transiently expresses golgi localized TurboID with HA tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX4T3-HA-ERK2 GOF

    Plasmid

    #53200

    Purpose

    Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S/D321N

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

38. lentiCRISPR-v2-HygR-mCherry

    Plasmid

    #167189

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SmR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHB-4

    Plasmid

    #140957

    Purpose

    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. ePPE

    Plasmid

    #183095

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mPA-GFP-LaminB1-10

    Plasmid

    #57141

    Purpose

    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

43. pcKv10.1

    Plasmid

    #85703

    Purpose

    mammalian expression Kv10.1

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Use

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PLEX_313 SLC31A1

    Plasmid

    #184493

    Purpose

    Lentivirus expression for SLC31A1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    SLC31A1 (SLC31A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA5/FRT/TO V5 HSPA2

    Plasmid

    #19512

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 2 (HSPA2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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47. MS2_GFP

    Plasmid

    #61764

    Purpose

    Expresses MS2 coat protein fused to GFP

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    MS2 coat protein fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLV-Azurite

    Plasmid

    #36086

    Purpose

    3rd generation lentiviral plasmid

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLV-REPORT(PGK/CMV)

    Plasmid

    #172330

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pQE81L-KOFP7

    Plasmid

    #160330

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2020 May 27;68(21):58

    Insert

    KOFP7
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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